Registration Dossier

Environmental fate & pathways

Biodegradation in water: screening tests

Currently viewing:

Administrative data

Link to relevant study record(s)

Referenceopen allclose all

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Justification for type of information:
REPORTING FORMAT FOR THE ANALOGUE APPROACH
See 'Attached justification'.
Reason / purpose:
read-across source
Related information:
Composition 1
GLP compliance:
yes (incl. certificate)
Test material information:
Composition 1
Specific details on test material used for the study:
TEST MATERIAL
- Name of test material: neohesperidin
- IUPAC name: 5-hydroxy-2-(3-hydroxy-4-methoxyphenyl)-4-oxo-3,4-dihydro-2H-chromen-7-yl 2-O-(6-deoxyhexopyranosyl)hexopyranoside
- Molecular formula: C28H34O15
- Molecular weight: 610.5606
- Smiles notation: COc1ccc(cc1O)C2CC(=O)c3c(O)cc(OC4OC(CO)C(O)C(O)C4OC5OC(C)C(O)C(O)C5O)cc3O2
- InChl: InChI= 1/C28H34O15/c1-10-21(33)23(35)25(37)27(39-10)43-26-24(36)22(34)19(9-29)42-28(26)40-12-6-14(31)20-15(32)8-17(41-18(20)7-12)11-3-4-16(38-2)13(30)5-11/h3-7,10,17,19,21-31,33-37H,8-9H2,1-2H3
Key result
Parameter:
% degradation (O2 consumption)
Sampling time:
28 d
Remarks on result:
other: ready biodegradable
Remarks:
Read-across from analogue substance (naringin).
Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Conclusions:
Based on the read-across approach, the target substance is expected to be readily biodegradable.
Executive summary:

A 28-day ready aerobic biodegradability test in an aerobic aqueous medium with manometric respirometry method was performed on the analogue substance naringin, according to OECD 301F / EC C.4 – D manometric respirometry methods (GLP study). 100 mg/l of test item was inoculated with activated sludge (30 mg/L SS) and incubated under aerobic conditions in a closed respirometer flask at constant temperature (22 ± 2ºC) for 28 days. A blank test, a procedure test with reference substance (sodium acetate) and a toxicity test were run in parallel. In a toxicity test, containing both the test item and a reference item, on the 14th test day the biodegradation (based on ThOD) attained 54.1%. Thus, the analogue substance is not inhibitory.At the 28th day of the test the measured aerobic biodegradation of the test item attained 74.8%. Thus, the analogue substance is readily biodegradable. Based on the read-across approach, the target substance is expected to be readily biodegradable.

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Justification for type of information:
REPORTING FORMAT FOR THE ANALOGUE APPROACH
See 'Attached justification'.
Reason / purpose:
read-across source
Related information:
Composition 1
GLP compliance:
yes (incl. certificate)
Test material information:
Composition 1
Specific details on test material used for the study:
TEST MATERIAL
- Name of test material: neohesperidin
- IUPAC name: 5-hydroxy-2-(3-hydroxy-4-methoxyphenyl)-4-oxo-3,4-dihydro-2H-chromen-7-yl 2-O-(6-deoxyhexopyranosyl)hexopyranoside
- Molecular formula: C28H34O15
- Molecular weight: 610.5606
- Smiles notation: COc1ccc(cc1O)C2CC(=O)c3c(O)cc(OC4OC(CO)C(O)C(O)C4OC5OC(C)C(O)C(O)C5O)cc3O2
- InChl: InChI= 1/C28H34O15/c1-10-21(33)23(35)25(37)27(39-10)43-26-24(36)22(34)19(9-29)42-28(26)40-12-6-14(31)20-15(32)8-17(41-18(20)7-12)11-3-4-16(38-2)13(30)5-11/h3-7,10,17,19,21-31,33-37H,8-9H2,1-2H3
Key result
Parameter:
% degradation (O2 consumption)
Sampling time:
28 d
Remarks on result:
other: ready biodegradable
Remarks:
Read-across from analogue substance (neohesperidin dihydrochalcone).
Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Conclusions:
Based on the read-across approach, the target substance is expected to be readily biodegradable.
Executive summary:

A 28-day ready aerobic biodegradability test in an aerobic aqueous medium with manometric respirometry method was performed on the analogue substance neohesperidin dihydrochalcone, according to OECD 301F / EC C.4 – D manometric respirometry methods (GLP study). 100 mg/l of test item was inoculated with activated sludge (30 mg/L SS) and incubated under aerobic conditions in a closed respirometer flask at constant temperature (22 ± 2ºC) for 28 days. A blank test, a procedure test with reference substance (sodium acetate) and a toxicity test were run in parallel. In a toxicity test, containing both the test item and a reference item, on the 14th test day the biodegradation (based on ThOD) attained 42.7%. Thus, the analogue substance is not inhibitory.At the 28th day of the test the measured aerobic biodegradation of the test item attained 71%. Thus, the analogue substance is readily biodegradable. Based on the read-across approach, the target substance is expected to be readily biodegradable.

Description of key information

Weight of evidence: Based on the read-across approach from two analogue substances, the target substance is readily biodegradable.

- Read-across from analogue substance. Source: Method according to OECD 301F / EU C.4 (Manometric respirometry), GLP study. At the 28th day of the test the measured aerobic biodegradation of the test item attained 74.8%. Thus, the test item is readily biodegradable. Based on the read-across approach, the target substance is expected to be readily biodegradable.

- Read-across from analogue substance. Source: Method according to OECD 301F / EU C.4 (Manometric respirometry), GLP study. At the 28th day of the test the measured aerobic biodegradation of the test item attained 71%. Thus, the test item is readily biodegradable. Based on the read-across approach, the target substance is expected to be readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable

Additional information

Weight of evidence:

- Read-across from analogue substance. A 28-day ready aerobic biodegradability test in an aerobic aqueous medium with manometric respirometry method was performed on the analogue substance naringin, according to OECD 301F / EC C.4 – D manometric respirometry methods (GLP study). 100 mg/l of test item was inoculated with activated sludge (30 mg/L SS) and incubated under aerobic conditions in a closed respirometer flask at constant temperature (22 ± 2ºC) for 28 days. A blank test, a procedure test with reference substance (sodium acetate) and a toxicity test were run in parallel. In a toxicity test, containing both the test item and a reference item, on the 14th test day the biodegradation (based on ThOD) attained 54.1%. Thus, the analogue substance is not inhibitory. At the 28th day of the test the measured aerobic biodegradation of the test item attained 74.8%. Thus, the analogue substance is readily biodegradable. Based on the read-across approach, the target substance is expected to be readily biodegradable.

- Read-across from analogue substance. A 28-day ready aerobic biodegradability test in an aerobic aqueous medium with manometric respirometry method was performed on the analogue substance neohesperidin dihydrochalcone, according to OECD 301F / EC C.4 – D manometric respirometry methods (GLP study). 100 mg/l of test item was inoculated with activated sludge (30 mg/L SS) and incubated under aerobic conditions in a closed respirometer flask at constant temperature (22 ± 2ºC) for 28 days. A blank test, a procedure test with reference substance (sodium acetate) and a toxicity test were run in parallel. In a toxicity test, containing both the test item and a reference item, on the 14th test day the biodegradation (based on ThOD) attained 42.7%. Thus, the analogue substance is not inhibitory. At the 28th day of the test the measured aerobic biodegradation of the test item attained 71%. Thus, the analogue substance is readily biodegradable. Based on the read-across approach, the target substance is expected to be readily biodegradable.