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Physical & Chemical properties

Water solubility

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Reference
Endpoint:
water solubility
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From May 19th to May 20th, 2016.
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 105 (Water Solubility)
Deviations:
no
Qualifier:
according to
Guideline:
EU Method A.6 (Water Solubility)
Deviations:
no
GLP compliance:
yes (incl. certificate)
Type of method:
column elution method
Key result
Water solubility:
3.98 mg/L
Conc. based on:
test mat.
Loading of aqueous phase:
74 g/L
Incubation duration:
2 h
Temp.:
20 °C
pH:
> 6.33 - < 6.37

Table 1. Results of the preliminary test.

0.01 g in x mL of water

0.05

0.1

0.2

1

10

100

200

1000

>1000

Soluble (+/-)

-

-

-

-

-

-

-

-

+

Approximate solubility,
g/L

> 200

200
to
100

100
to
50

50
to
10

10
to
1

1
to
0.1

0.1
to
0.05

0.05
to
0.01

< 0.01

Table 2. Results of test item solubility at 20 ± 0.5ºC in distilled water, column elution with recirculating pump method.

 

1

2

3

4

5

Average
concentration of
Neohesperidin on
successive runs,
mg/L

Average
concentration of
Neohesperidin,
mg/L

1
run

Concentration of
Neohesperidin,
mg/L

3.79

3.98

3.88

4.01

3.98

3.93 ± 0.09

3.98 ± 0.07

pH

6.34

6.34

6.35

6.33

6.34

2
run

Concentration of
Neohesperidin,
mg/L

4.03

4.07

3.99

4.03

4.01

4.03 ± 0.03

pH

6.36

6.34

6.35

6.35

6.37

Conclusions:
The solubility of the test item in water is 3.98 ± 0.07 mg/L, at 20ºC, pH 6.33-6.37
Executive summary:

The study of the water solubility of the test item was performed by the column elution method, according to OECD 105 / EU Method A.6 (GLP study). The test item was dissolved in acetone and added to innert support material. Then, the solvent was evaporated, and the loaded support material was soaked for 2h in 5 ml of water. This suspension was added to a micro-column and the test item was eluted until the concentration reached a plateau. The mass concentration of the eluate at this time was determined by UV-VIS spectrophotometry, and taken as the water solubility value. Based on the test results, the solubility of the test item in water was found to be 3.98 ± 0.07 mg/L, at 20ºC, pH 6.33 - 6.37.

Description of key information

Key study. Column elution method, according to OECD 105 / EU Method A.6, GLP study. The solubility of the test item in water was found to be 3.98 ± 0.07 mg/L, at 20ºC, pH 6.33 - 6.37.

Key value for chemical safety assessment

Water solubility:
3.98 mg/L
at the temperature of:
20 °C

Additional information

The study of the water solubility of the test item was performed by the column elution method, according to OECD 105 / EU Method A.6 (GLP study). The test item was dissolved in acetone and added to innert support material. Then, the solvent was evaporated, and the loaded support material was soaked for 2h in 5 ml of water. This suspension was added to a micro-column and the test item was eluted until the concentration reached a plateau. The mass concentration of the eluate at this time was determined by UV-VIS spectrophotometry, and taken as the water solubility value. Based on the test results, the solubility of the test item in water was found to be 3.98 ± 0.07 mg/L, at 20ºC, pH 6.33 - 6.37.