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Toxicological information

Acute Toxicity: dermal

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Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Range-finding toxicity data: list IV
Author:
Smyth HF Jr, Carpenter CP and Weil CS
Year:
1951
Bibliographic source:
Arch. Ind. Hyg. Occup. Med. 4, 119-122
Reference Type:
study report
Title:
Unnamed
Year:
1949
Report Date:
1949

Materials and methods

Principles of method if other than guideline:
Smyth HF Jr, Carpenter CP (1944): The place of the range finding test in the industrial toxicology laboratory. J. Ind. Hyg. Toxicol. 26, 269-273 and subsequent updates
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Tetrahydronaphthalene procured from Eastman Kodak Co. under their number P550 on 10 Jan. 1949. No data on purity

Test animals

Species:
rabbit
Strain:
not specified
Sex:
male
Details on test animals and environmental conditions:
TEST ORGANISMS: 
-male Albino Rabbbits 
-Weight at study initiation: 2,202-2,718 g

Administration / exposure

Type of coverage:
semiocclusive
Vehicle:
other: undiluted
Details on dermal exposure:
ADMINISTRATION: 
- Area covered: clipped trunk
- Occlusion: impervious flexible film ("vinylite" sheeting)
- Exposure period: 24 hours 
- Observation period: 14 days
Duration of exposure:
24 hours
Doses:
12.6; 15.8; 20.0 g/kg bw
No. of animals per sex per dose:
5
Control animals:
no
Statistics:
Thompson, W.R. (1947): Use of moving averages and  interpolation to estimate median effective dose. Bact. Rev. 11, 115

Results and discussion

Effect levels
Sex:
male
Dose descriptor:
LD50
Effect level:
ca. 16 800 mg/kg bw
Mortality:
MORTALITY:   
 LD50 = 17.3 (14.5 - 20.6) mL/kg bw
    = 16.8 (14.1 - 20.0)  g/kg bw
Clinical signs:
CLINICAL SIGNS: 
Upon removal of the covering the skin was erythematous,  on subsequent examination it was necrosed and ultimately leathery and dry.
Gross pathology:
Autopsy revealed pale livers and kidneys and  congestion of the pancreas and intestines.

Any other information on results incl. tables

- Number of deaths at each dose: 
  12,600 mL/kg bw:  1/5 on day 4
  15,800 mL/kg bw:  1/5 on day 13
  20,000 mL/kg bw:  1+2+1/5 on days 11, 12, 14

Applicant's summary and conclusion

Conclusions:
The LD50 (rabbit) for tetrahydronaphthalene applied to the skin of the rabbit is 17.3 mL/kg bw (=16.8 g/kg bw). Thus tetrahydronaphthalene showed low acute toxicity in rabbits after dermal application.
Executive summary:

The LD50 for tetrahydronaphthalene applied undiluted to the clipped skin of the rabbit trunk under "Vinylit" sheeting for 24h was 17.3 mL/kg bw (14.5 -20.6). Upon removal of the covering the the skin was erythemous, on subsequent examination it was necrosed and ultimately because leathery and dry. Autopsy of victims revealed pale livers, and kidneys and congestion of the pancreas and intestines. The disparity between oral and percutaneous toxicity indicates that tetrahydronaphthalene pentrates quite slowly through the skin.