1,2,3,4-tetrahydronaphthalene

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study well documented, meets generally accepted scientific principles, acceptable for assessment. Restriction: Only male animals used

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Range-finding toxicity data: list IV

Author:

Smyth HF Jr, Carpenter CP and Weil CS

Year:

1951

Bibliographic source:

Arch. Ind. Hyg. Occup. Med. 4, 119-122

Reference 2

Reference Type:

study report

Title:

Unnamed

Year:

1949

Report date:

1949

Materials and methods

Principles of method if other than guideline:

Method: other: Smyth HF Jr, Carpenter CP (1944): The place of the range finding test in the industrial toxicology laboratory. J. Ind. Hyg. Toxicol. 26, 269-273 and subsequent updates

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

1,2,3,4-tetrahydronaphthalene

EC Number:

204-340-2

EC Name:

1,2,3,4-tetrahydronaphthalene

Cas Number:

119-64-2

Molecular formula:

C10H12

IUPAC Name:

1,2,3,4-tetrahydronaphthalene

Details on test material:

tetrahydronaphthalene procured from Eastman Kodak Co. under their number P550 on 10 Jan. 1949. No data on purity

Test animals

Species:

rat

Strain:

Sherman

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ORGANISMS: 
- Weight at study initiation: 90-120 g
- Feeding: No previous withdrawal of food

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

water

Details on oral exposure:

ADMINISTRATION: 
- Vehicle: Test substance fed by stomach tube as a 20% dispersion in 1%  "Tergitol" 7
- Post dose observation period: 14 days

Doses:

2,000; 2,520; 3,160; 3,980; 7,950 mg/kg bw, maybe additional doses

No. of animals per sex per dose:

10

Control animals:

not specified

Statistics:

Thompson, W.R. (1947): Use of moving averages and  interpolation to estimate median effective dose. Bact. Rev. 11, 115

Results and discussion

Effect levels

Key result

Sex:

male

Dose descriptor:

LD50

Effect level:

ca. 2 860 mg/kg bw

Mortality:

MORTALITY: LD50 = 2.86 (2.58-3.18) g/kg bw

Clinical signs:

other: CLINICAL SIGNS:  Following the doses, the rats exhibited symptoms of sluggishness, prostration, and narcosis. The urine had a brownish coloration.

Gross pathology:

NECROPSY FINDINGS: 
A dose of 7950 mg/kg bw produced severe lung hemorrhage, congestion of the liver, paleness of the kidney with edema in  some instances, 
opacity and adhesions of the intestines. Several of the  livers were jaundiced after the administration of a dosage of 3980 mg/kg  bw. This effect 
was not found at the higher level because of rapid death.  Lower dosage levels produced similar symptoms of lesser intensity.

Any other information on results incl. tables

MORTALITY: LD50 = 2.86 (2.58-3.18) g/kg bw
- Time of death: often delayed 3 to 4 days
- Number of deaths at each dose: 
  2,000 mg/kg bw:  1/10 (day 5)
  2,520 mg/kg bw:  2/10 (day 4)
  3,160 mg/kg bw:  3+2+2/10 (days 1, 3, 4)
  3,980 mg/kb bw:  3+2+3+2/10 (days 1, 2, 3, 4)

Applicant's summary and conclusion

Conclusions:

The feeding of the test substance to male albino strain rats resulted in a LD50 =2.86 g/kg bw (2.58 -3.18) based on a 14 day observation period. Thus tetrahydronaphthalene showed low acute toxicity in rats after oral gavage.

Executive summary:

The feeding of tetrahydronaphthalene to male albino strain rats, without previous withdrawal of food, as a 20% dispersion in 1% "Tergitol" resulted in a LD50 =2.86 g/kg bw (2.58 -3.18) based on a 14 day observation period. Clinical signs included sluggishness, prostration and narcosis with deaths often delayed 3 to 4 days. The highest dose of 7.95 g/kg bw produced severe lung hamorrhage, congestion of the liver, paleness of the kidney with edema in some instances, opacity and adhesions of the intestines. Several of the livers were jaundiced after the administration of a dosage of 3980 mg/kg bw. This effect was not found at the higher level because of rapid death. Lower dosage levels produced similar symptoms of lesser intensity.