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Toxicological information

Specific investigations: other studies

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Administrative data

Endpoint:
cytotoxicity
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
The cytotoxicity of some organic solvents
Author:
Holmberg B and Malmfors T
Year:
1974
Bibliographic source:
Environ. Res. 7, 183-192

Materials and methods

Type of method:
in vitro

Test material

Reference
Name:
Unnamed
Type:
Constituent

Test animals

Details on test animals and environmental conditions:
Ehrlich-Landschütz  diploid (ELD) ascites tumour cells propagated in outbred albino NMRI mice

Administration / exposure

Details on exposure:
TC-Freetext:
- Test vessel: sealed 3 ml glass tubes filled to the top
- Test concentrations: 25, 50, 75, or 100 ppm (= mg/l)
- Test temperature: 37 +/- 1 degree C
- Aeration: No; constant stirring
- No. of replicates: one per concentration and observation
- Observation: after 0, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5 h
- Evaluation: counting of 200 cells per sample after staining with 2 %  Lissamine green B
Details on study design:
Cytotoxicity of tetrahydronaphthalene was studied in Ehrlich-Landschütz  diploid (ELD) ascites tumour cells propagated in outbred albino NMRI mice.

Results and discussion

Any other information on results incl. tables

RS-Freetext:
Percent of irreversibly injured cells:
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Time   0 mg/l   25 mg/l   50 mg/l   75 mg/l   100 mg/l
------------------------------------------------------
 0 h    2.3       3.0       3.0       3.0        4.0
 1 h    2.6       2.5       5.5       4.5       11.0
 2 h    3.1       6.5      15.0      17.0       41.5
 3 h    3.6       8.0      16.0      17.5       40.5
 4 h    3.5      15.0      18.0      24.5       67.0
 5 h    4.2        ?       18.5        ?        95.5
------------------------------------------------------
1,2,3,4-tetrahydronaphthalene was assigned a high toxicity towards ELD  cells.

Applicant's summary and conclusion