Reaction product of acid red 143 (free acid) or the respective sodium salt (e.g Lansyn red F-5B) (UVCB) with Tetrabutylammonium bromide (CAS nr.: 1643-19-2) resulting in a mixture of AR143-(TBN)2 (Main component), AR143-(TBN), AR143-(TBN)3, AR143-(TBN) without alkyl tail, AR143-(TBN)2 without alkyl tail.Chemical name main component AR143-(TBN)2:di-(tetra-n-butylammonium) salt of 1-benzoyl-4-[4'-(1'',1''-dimethylpropyl)-phenoxy]-6-phenylamino-2,7-dioxo-2,7-dihydro-3H-naphtho(1,2,3-de)quinolin, disulfonic acid

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

15 Mar 2017 – 29 Mar 2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

1

Reference substance name:

Reaction product of acid red 143 (free acid) or the respective sodium salt (e.g Lansyn red F-5B) (UVCB) with Tetrabutylammonium bromide (CAS nr.: 1643-19-2) resulting in a mixture of AR143-(TBN)2 (Main component), AR143-(TBN), AR143-(TBN)3, AR143-(TBN) without alkyl tail, AR143-(TBN)2 without alkyl tail. Chemical name main component AR143-(TBN)2: di-(tetra-n-butylammonium) salt of 1-benzoyl-4-[4'-(1'',1''-dimethylpropyl)-phenoxy]-6-phenylamino-2,7-dioxo-2,7-dihydro-3H-naphtho(1,2,3-de)quinolin, disulfonic acid

EC Number:

944-248-6

Molecular formula:

Combinations of mono (C40H31N2O7S1) bi (C40H30N2O10S2) and trivalent (C40H29N2O13S3) AR143 with TBN (C16H36N+)

IUPAC Name:

Reaction product of acid red 143 (free acid) or the respective sodium salt (e.g Lansyn red F-5B) (UVCB) with Tetrabutylammonium bromide (CAS nr.: 1643-19-2) resulting in a mixture of AR143-(TBN)2 (Main component), AR143-(TBN), AR143-(TBN)3, AR143-(TBN) without alkyl tail, AR143-(TBN)2 without alkyl tail. Chemical name main component AR143-(TBN)2: di-(tetra-n-butylammonium) salt of 1-benzoyl-4-[4'-(1'',1''-dimethylpropyl)-phenoxy]-6-phenylamino-2,7-dioxo-2,7-dihydro-3H-naphtho(1,2,3-de)quinolin, disulfonic acid

Test material form:

solid

Details on test material:

- Stabilisation: stable
- Safety precautions: The routine hygienic procedures were sufficient to assure personnel health and safety
- Colour: brownish red
- Storage conditions: room temperature

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Analytical samples taken at 0 hours (initial value) and 24 hours from fresh and 48 h aged test solutions were analysed from control and all test item concentrations.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna Straus, Clone V, was used as the test organism. The animals are continuously bred in the laboratory and were originally purchased in a healthy condition from the Federal Environment Agency in Berlin/Germany.
Daphnia magna was bred as single culture (1 daphnid per 100 mL) in Elendt M4 medium. The pH- value of the aerated water was within a range of 6.0 – 9.0. The dissolved oxygen was above 60 % saturation and the total hardness 140 - 250 mg/L (as CaCO3), corresponding to 7.8 - 14°dH. The animals were fed with single cell green algae (Desmodesmus subspicatus, formerly Scenedesmus subspicatus) at least three times a week.
The daphnids were reared at a temperature of 20 ± 2 °C in a climatic chamber with 16 hours of illumination and 8 hours of darkness. The medium was changed three times per week. A pipette was used to separate the young daphnids from the adults.
Freshly hatched daphnids less than 24 hours old were used for the test.

Study design

Test type:

semi-static

Water media type:

freshwater

Total exposure duration:

48 h

Remarks on exposure duration:

Endpoints reported are the EC50 and the NOEC after 24 and 48 hours

Test conditions

Hardness:

The total hardness (as CaCO3) of the untreated control was determined to be 12°dH (214 mg/L CaCO3)

Test temperature:

19.7 – 21.0 °C

pH:

7.76 – 8.15

Dissolved oxygen:

The dissolved oxygen concentration at the end of the test should be 3 mg/L in all test units. In this test, the dissolved oxygen concentration at the end of the test was 8.6 mg/L.

Details on test conditions:

A stock solution (1300 mg/L, purity not considered), and a dilution (4 mg/L) was prepared in methanol. The stock solution was used for fortification of 13 mg/L recovery samples, the dilution was used for fortification of 0.07 mg/L recovery samples.

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

ca. 2.68 mg/L

Nominal / measured:

nominal

Effect concentrations 2

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

ca. 0.427 mg/L

Nominal / measured:

nominal

Details on results:

According to the results of the test, the EC50 (48 h) for immobilisation was determined to be
2.68 mg/L (nominal) corresponding to 1.93 mg/L (actual). The corresponding NOEC (48 h) was 0.427 mg/L (nominal) corresponding to 0.307 mg/L (actual).

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

According to the results of the test, the EC50 (48 h) was determined to be 2.68 mg/L (nominal) corresponding to 1.93 mg/L (actual). The corresponding NOEC (48 h) was 0.427 mg/L (nominal) corresponding to 0.307 mg/L (actual).