Melaleuca alternifolia, ext.

Boiling point

Administrative data

Endpoint:

boiling point

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2007

Report date:

2007

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method A.2 (Boiling Temperature)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 103 (Boiling point/boiling range)

Deviations:

no

Test guideline 3

Qualifier:

according to guideline

Guideline:

EPA OPPTS 830.7220 (Boiling Point / Boiling Range)

Deviations:

no

GLP compliance:

yes

Type of method:

distillation method

Test material

Test material information

Constituent 1

Reference substance name:

Melaleuca alternifolia, ext.

EC Number:

285-377-1

EC Name:

Melaleuca alternifolia, ext.

Cas Number:

85085-48-9

Molecular formula:

Not applicable (a generic molecular formula cannot be provided for this specific UVCB substance).

IUPAC Name:

Essential oil of melaleuca alternifolia

Test material form:

liquid

Specific details on test material used for the study:

- Purity: 100%
- Lot (batch) No.: 1215
- Expiration date of the lot: 30 March 2009
- Storage condition of test material: Room temperature, protected from light.

Results and discussion

Boiling point

Boiling pt.:

>= 97 - <= 220 °C

Atm. press.:

>= 1 004 - <= 1 007 mBar

Remarks on result:

other: The atmospheric pressure readings for the duplicate determinations were 1004 and 1007 mbar respectively. Consequently no pressure correction was required to the determined temperature values.

Any other information on results incl. tables

The boiling temperatures (temperature corrected for the thermometer and to Standard Pressure, recorded at 10 ml intervals) are shown in Table 1 below:

Table 1. Boiling temperatures.

Volume distilled (ml)	Distillation temperature (°C)
	Run 1	Run 2	Mean
Initial distillation	96	97	97
5	175	179	177
10	187	188	188
20	190	191	191
30	191	191	191
40	191	191	191
50	194	192	193
60	196	197	197
70	200	200	200
80	208	206	207
90	214	214	214
95	219	221	220
Dry point	219	219	219

A dark yellow solid remained at the end of each test.

Applicant's summary and conclusion

Conclusions:

The boiling range of Tea Tree Oil was found to be 97 to 220°C.

Executive summary:

A GLP-compliant study was carried out to determine the boiling temperature of Tea Tree Oil. The study followed the requirements of EEC method A2, OECD method 103 and OPPTS method 830.7220 without significant deviation. The boiling range of Tea Tree Oil was found to be 97 to 220°C.