Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 233-311-7 | CAS number: 10114-47-3
Table 1: Immobolization of daphnia
Number of mobile animals
24 hours/series 1
24 hours/series 2
48 hours/series 1
48 hours/series 2
The test substance, Direct Yellow 28, was tested in acute immobilisation test on Daphnia magna. The test was performed according to method C.2 – Daphnia sp. Acute Immobilisation Test, Council Regulation (EC) No. 440/2008, published in O.J. L 142, 2008.
The preliminary test was performed in a range of the test substance nominal concentrations 1 – 100 mg/L. The test was performed as static. Based on no toxicity of the test substance found in the preliminary test, the study was terminated. The experiment with the concentration of 100 mg/L included in the preliminary test has been performed using 20 daphnia, therethrough the conditions of the limit test given in guideline have been fulfilled. It was demonstrated that the EC50 is higher than this concentration.
The samples for analytical determination were taken at the beginning and at the end of the test. The analytical results from the preliminary test showed, that the test substance, Direct Yellow 28, was stable in the dilution water at conditions of the test.
There is stated in the guideline that, if evidence is available to demonstrate that the concentration of the test substance has been satisfactorily maintained within ± 20 percent of the nominal or measured initial concentration throughout the test, the results can be based on nominal or measured initial values.
The nominal concentrations were used for all evaluation and results.
EC50 > 100 mg/L (24 h, Daphnia magna) (nominal concentration)
EC50 > 100 mg/L (48 h, Daphnia magna) (nominal concentration)
EC0 = 100 mg/L (24 h, Daphnia magna) (nominal concentration)
EC0 = 100 mg/L (48 h, Daphnia magna) (nominal concentration)
EC100 > 100 mg/L (24 h, Daphnia magna) (nominal concentration)
EC100 > 100 mg/L (48 h, Daphnia magna) (nominal concentration)
The effective concentration EC50 > 100 mg/L was determined based on immobilisation of daphnia.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Close Do not show this message again