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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
key study
Study period:
July 1973
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1973
Report date:
1973

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 402 (Acute Dermal Toxicity)
Principles of method if other than guideline:
- Principle of test: In this limit test, a high dose of 5000 mg.kg bw Cedarwood Virginia was applied dermally to the skin of 9 rabbits. The rabbits were observed for 14 days.
GLP compliance:
no
Remarks:
pre GLP
Test type:
standard acute method
Limit test:
yes

Test material

Constituent 1
Reference substance name:
Juniper, Juniperus virginiana, ext.
EC Number:
285-370-3
EC Name:
Juniper, Juniperus virginiana, ext.
Cas Number:
85085-41-2
Molecular formula:
Not available since an UVBC substance.
IUPAC Name:
Essential oil of Cedarwood obtained from the wood of Juniperus virginiana (Cupressaceae) by steam distillation
Test material form:
liquid: viscous
Details on test material:
CAS 85085-41-2
For specific details on test material if available - See in RSS
Name of test material (as cited in study report or in reference): Cedarwood Virginia oil; Cedarwood oil Virginia or Virginia oil
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- No.of test material: Sample marking: 73-9

Test animals

Species:
rabbit
Strain:
New Zealand White
Sex:
not specified

Administration / exposure

Type of coverage:
not specified
Vehicle:
unchanged (no vehicle)
Details on dermal exposure:
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 5000 mg/kg bw
- Concentration (if solution): undiluted
Duration of exposure:
not specified
Doses:
Limit dose: 5000 mg/kg bw
No. of animals per sex per dose:
9
Control animals:
not specified
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations : daily
- Necropsy of survivors performed: not specified
- Other examinations performed: Skin irritation, others not specified

Results and discussion

Effect levels
Key result
Sex:
not specified
Dose descriptor:
LD50
Effect level:
> 5 000 mg/kg bw
Based on:
test mat.
Mortality:
None
Clinical signs:
other: Slight redness - 7/9 Moderate redness - 1/9 Slight edema - 3/9 Moderate edema - 6/9
Gross pathology:
not specified
Other findings:
not specified

Applicant's summary and conclusion

Interpretation of results:
other: not classified
Remarks:
based on CLP criteria
Conclusions:
The dermal LD50 of Cedarwood Virginia were determined to be higher than 5000 mg/kg bw. Based on the available information, the test substance does not need to be classified for acute dermal toxicity, according to the classification criteria outlined in Annex I of 1272/2008/EC (CLP).
Executive summary:

In this limit test, a high dose of 5000 mg.kg bw Cedarwood Virginia was applied dermally to the skin of 9 rabbits. The rabbits were observed for 14 days. No mortality was observed, and the LD50 was established to be higher than 5000 mg/kg bw. Based on the available information, the test substance does not need to be classified for acute dermal toxicity, according to the classification criteria outlined in Annex I of 1272/2008/EC (CLP)