Reaction product of "Fatty acids, C16-18 (even numbered) and C18-unsaturated" and "reaction mass of 1,3-alkanediol, 2-(hydroxymethyl)-2-[(methoxymethoxy)methyl]- and 1,3-heteromonocycle-5,5-dimethanol

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

August 03, 2007

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: The study has been performed according to OECD guidelines and according to GLP principles.

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)

Deviations:

no

Principles of method if other than guideline:

ISO Standard 8192, Water Quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation (2007).

GLP compliance:

yes

Analytical monitoring:

no

Test organisms (species):

activated sludge of a predominantly domestic sewage

Test type:

other: aerobic

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

3 h

Key result

Duration:

3 h

Dose descriptor:

IC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Key result

Duration:

3 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Comments:
Under the conditions of this present test, the test
substance was not toxic to waste water (activated sludge)
bacteria at the loading rate of 100 mg/l, the regulatory
limit concentration.

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions of this present test, Reaction products of Fatty acids, C16-18 and C18-unsatd. and reaction mass of 1,3-alkanediol, 2-(hydroxymethyl)-2-[(methoxymethoxy)methyl]- and 1,3-heteromonocycle-5,5-dimethanol was not toxic to waste water (activated sludge) bacteria at the loading rate of 100 mg/l, the regulatory limit concentration.

Description of key information

The study was performed according to OECD Guideline 209 and GLP principles.

Key value for chemical safety assessment

EC50 for microorganisms:

100 mg/L

EC10 or NOEC for microorganisms:

100 mg/L

Additional information

The respiration rate of activated sludge was investigated according to OECD test guideline 209. The sludge was exposed to Radia 7853 with a contact time of 3 hours, at a final loading rate of 100 mg/l. No significant inhibition of respiration rate of the sludge was recorded at the loading rate of 100 mg/l, thus no further testing was required. Hence, the EC50 of Rqadia 7853 was >100 mg/l (nominal) and the NOEC was determined to be 100 mg/l.