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Environmental fate & pathways

Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From January 25, 1996 to April 15, 1996
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
other: Annex to Commission Directive 92/69/EEC of 31 July 1992. C.4-C. L 383 A/1202. OECD Guidelines for the Testing of Chemicals, 301 B, 1992 (Modified Sturm Test).
Deviations:
no
GLP compliance:
yes
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Oxidation ditch in the municipality of Berkel Rodenrijs, the Netherlands. The ditch is used to treat domestiv sewage.
- Pretreatment: The original sludge (3.0 g of solid substance per L) was mixed and 250 mL of the sludge was used to inoculate 25 litres of medium. The medium and the inoculum were aerated with CO2-free air for about 20h before use.
- Concentration of sludge: 30 mg/L solid substances
Duration of test (contact time):
29 d
Initial conc.:
11.5 mg/L
Based on:
TOC
Initial conc.:
23 mg/L
Based on:
TOC
Parameter followed for biodegradation estimation:
CO2 evolution
Reference substance:
acetic acid, sodium salt
Key result
Parameter:
% degradation (CO2 evolution)
Value:
ca. 89
Sampling time:
28 d
Parameter:
% degradation (CO2 evolution)
Value:
51
Sampling time:
7 d
Parameter:
% degradation (CO2 evolution)
Value:
74
Sampling time:
14 d
Results with reference substance:
Points of degradation plot (reference substance):
44 % degradation after 7 d
66 % degradation after 14 d
93 % degradation after 28 d
Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Conclusions:
Laccase is readily biodegradable according to OECD 301B.
Executive summary:

A ready biodegradability test (OECD 301B, Modified Sturm test) was performed to determine the biodegradability characteristics of the test substance, Laccase SP 807. The study was conducted according to OECD and EC guidelines and was performed in accordance with GLP. The results obtained for the degradation of sodium acetate (reference substance) showed the validity of this test. Mixtures containing the test substance showed degradation to an extent of 51% after seven days, 74% after 14 days, and 89% by the end of the test after 28 days. On this basis the test substance was considered readily biodegradable under the conditions of the test.

Description of key information

The test substance was considered to be readily biodegradable under the conditions of the ready biodegradation test (OECD test 301B). The test substance reached 89% degradation by the end of the 28 day test period.

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable

Additional information

A ready biodegradability test (OECD 301B, Modified Sturm test) was performed to determine the biodegradability characteristics of the test substance. The study was conducted according to OECD and EC guidelines and was performed in accordance with GLP. The results obtained for the degradation of sodium acetate (reference substance) showed the validity of this test. Mixtures containing the test substance showed degradation to an extent of 51% after seven days, 74% after 14 days, and 89% by the end of the test after 28 days. On this basis the test substance was considered readily biodegradable under the conditions of the test.