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Short term toxicity to aquatic invertebrates:

Based on the prediction done using the OECD QSAR toolbox version 3.4 with log kow as the primary descriptor and considering the six closest read across substances, toxicity on Daphnia magna predicted for 4-hydroxy-7-[(5-hydroxy-7-sulfonaphthalen-2-yl)amino]naphthalene-2-sulfonic acid (87 -03 -6). The EC50 value was estimated to be 332.83 mg/l when 4-hydroxy-7-[(5-hydroxy-7-sulfonaphthalen-2-yl)amino]naphthalene-2-sulfonic acid exposed to Daphnia magna for 48 hrs.

 

Short term toxicity to aquatic algae and cyanobacteria:

Using the OECD QSAR toolbox version 3.4 with log kow as the primary descriptor and considering the six closest read across substances, the short term toxicity on aquatic algae and cyanobacteria was predicted for target substance 4-hydroxy-7-[(5-hydroxy-7-sulfonaphthalen-2-yl)amino]naphthalene-2-sulfonic acid (CAS no. 87-03-6). EC50 value was estimated to be 177.0217 mg/l for Pseudokirchneriella subcapitata for 72 h duration. Based on this value it can be concluded that the substance 4-hydroxy-7-[(5-hydroxy-7-sulfonaphthalen-2-yl)amino] naphthalene-2-sulfonic acid is considered to be non-toxic to aquatic environment and can be considered to be not classified as per the CLP classification criteria.

Additional information

Summarized result of toxicity of 4-hydroxy-7-[(5-hydroxy-7-sulfonaphthalen-2-yl) amino]naphthalene-2-sulfonic acid (CAS no. 87-03-6) on the growth and other activity of aquatic invertebrates, algae and cyanobacteria by considering the data for target as well as RA chemical which was selected on the basis of log Kow as the primary descriptor are as follows:  

 

Short term toxicity to aquatic invertebrates:

Various predicted data for the target compound 4-hydroxy-7-[(5-hydroxy-7-sulfonaphthalen-2-yl) amino]naphthalene-2-sulfonic acid (CAS no. 87-03-6) and supporting weight of evidence studies for its closest read across substance with log Kow as the primary descriptor were reviewed for the toxicity on the invertebrates end point which are summarized as below:

 

In a prediction done by SSS (2017), Based on the prediction done using the OECD QSAR toolbox version 3.4 with log kow as the primary descriptor and considering the six closest read across substances, toxicity on Daphnia magna predicted for 4-hydroxy-7-[(5-hydroxy-7-sulfonaphthalen-2-yl)amino]naphthalene-2-sulfonic acid (87 -03 -6). The EC50 value was estimated to be 332.83 mg/l when 4-hydroxy-7-[(5-hydroxy-7-sulfonaphthalen-2-yl)amino]naphthalene-2-sulfonic acid exposed to Daphnia magna for 48 hrs.

 

In second prediction using the Estimation Programs Interface Suite (EPI suite, 2017), toxicity measured. Based on the prediction done by EPI suite, ECOSAR version 1.1, on the basis of similarity of structure to chemicals for which the aquatic toxicity has been previously measured by structure-activity relationships (SARs) program, the LC 50 value for short term toxicity to aquatic invertebrates was predicted. On the basis of this program, the LC 50 value for short term toxicity to aquatic invertebrates was predicted to be 3537.372 mg/l for 4-hydroxy-7-[(5-hydroxy-7-sulfonaphthalen-2-yl) amino]naphthalene-2-sulfonic acid in 48 hrs. Based on this value it can be concluded that the substance 4-hydroxy-7-[(5-hydroxy-7-sulfonaphthalen-2-yl) amino]naphthalene-2- sulfonic acid is considered to be not toxic to aquatic environment and cannot be classified as per the criteria mentioned in CLP regulation.

 

Similarly in a supporting weight of evidence study from ECOTOX database 2017 for read across chemical Isopropylamine (75-31-0), toxicity experiment was conducted for evaluating the effect of read across substance. Determination of short term toxicity of Isopropyl amine on the mobility of daphnia magna for 48hrs. Test performed by static system. After the exposure effect concentration was measured on the basis of intoxication were effect measured by the immobility of daphnia magna. Based on the immobilization of daphnia magna due to the exposure with chemical for 48 hrs, the EC50 was 197.6 mg/l. Based on the EC50, it can be concluded that the chemical was nontoxic and can be consider to be not classified as toxic as per the CLP classification criteria.

 

On the basis of above results for target chemical 4-hydroxy-7-[(5-hydroxy-7-sulfonaphthalen-2-yl) amino]naphthalene-2-sulfonic acid (CAS no. 87-03-6) (from OECD QSAR toolbox version 3.3 and EPI suite, 2017) and for its read across substance from ECOTOX database it can be concluded that the test substance 4-hydroxy-7-[(5-hydroxy-7-sulfonaphthalen-2-yl) amino]naphthalene-2-sulfonic acid is not toxic and can be consider to be not classified as toxic as per the CLP classification criteria.

Short term toxicity to aquatic algae and cyanobacteria:

Various predicted data for the target compound 4-hydroxy-7-[(5-hydroxy-7-sulfonaphthalen-2-yl) amino]naphthalene-2-sulfonic acid (CAS no. 87-03-6) and supporting weight of evidence studies for its closest read across substance with log Kow as the primary descriptor were reviewed for the toxicity end point which are summarized as below:

 

In a prediction done by SSS (2017) Using the OECD QSAR toolbox version 3.4 with log kow as the primary descriptor and considering the six closest read across substances, the short term toxicity on aquatic algae and cyanobacteria was predicted for target substance 4-hydroxy-7-[(5-hydroxy-7-sulfonaphthalen-2-yl)amino]naphthalene-2-sulfonic acid (CAS no. 87-03-6). EC50 value was estimated to be 177.0217 mg/l for Pseudokirchneriella subcapitata for 72 h duration. Based on this value it can be concluded that the substance 4-hydroxy-7-[(5-hydroxy-7-sulfonaphthalen-2-yl)amino] naphthalene-2-sulfonic acid is considered to be non-toxic to aquatic environment and can be considered to be not classified as per the CLP classification criteria.

 

In second prediction using the Estimation Programs Interface Suite (EPI suite, 2017), toxicity measured. Based on the prediction done using the EPI Suite ECOSAR version 1.10, the short term toxicity on green algae was predicted for test substance 4-hydroxy-7-[(5-hydroxy-7 -sulfo- naphthalen-2-yl)amino]naphthalene-2-sulfonic acid (87-03 -6). On the basis of effects observed in a static freshwater system, the effect concentration EC50 value for the substance 4-hydroxy-7-[(5-hydroxy-7-sulfonaphthalen-2-yl)amino]naphthalene-2-sulfonic acid is estimated to be 1547.719 mg/l for green algae for 96 hrs duration. Based on this value, it can be concluded that the test chemical 4-hydroxy-7-[(5-hydroxy-7-sulfonaphthalen-2-yl)amino]naphthalene -2-sulfonic acid can be considered as non-toxic to green algae at environmentally relevant concentrations and can be considered not-classified as toxic as per the CLP classification criteria.

 

Similarly in a supporting weight of evidence study from ECOTOX database 2017 for read across chemical Isopropylamine (75-31-0), Determination of short term toxicity of Isopropyl amine on the population of green algae (Pseudokirchneriella Subcapitata) for 4 days of exposure. Test performed by static system where 8.0 E+4 CELLS/ML was present in the vessel and exposed with the chemical for 96 hrs. (4 days). After the exposure inhibitory concentration was measured on the basis of changes in the population. Based on the changes in population of green algae (Pseudokirchneriella Subcapitata) due to the exposure with chemical for 96 hrs, the IC50 was 118.4 mg/l. Based on the IC50, it can be concluded that the chemical was nontoxic and can be consider to be not classified as toxic as per the CLP classification criteria.

 

Similarly in a supporting weight of evidence study from ECOTOX database 2017, for the read across chemical Butan-1-amine (CAS no. 109-73-9), toxicity experiment was conducted for evaluating the effect of read across substance. Short term toxicity study of chemical Butan-1-amine on green algae Chlorococcales was studied for 1 day. Study was conducted by static system for 24 hrs. Based on the Physiology and Assimilation efficiency of green algae (Chlorococcales), the EC10 was 1000 mg/l after the exposure of 1 day. Thus based on the EC10, it can be concluded that test chemical Butan-1-amine is not toxic and can be consider to be not classified as per the CLP classification criteria.

 

On the basis of above results for target chemical 4-hydroxy-7-[(5-hydroxy-7-sulfonaphthalen-2-yl) amino]naphthalene-2-sulfonic acid (CAS no. 87-03-6) (from OECD QSAR toolbox version 3.3 and EPI suite, 2017) and for its read across substance from ECOTOX database it can be concluded that the test substance 4-hydroxy-7-[(5-hydroxy-7-sulfonaphthalen-2-yl) amino]naphthalene-2-sulfonic acid is not toxic and can be consider to be not classified as per the CLP classification criteria.

 

Thus based on the data for short term toxicity to invertebrates and algae, database it can be concluded that the test substance 4-hydroxy-7-[(5-hydroxy-7-sulfonaphthalen-2-yl) amino]naphthalene-2-sulfonic acid is not toxic and can be consider to be not classified as per the CLP classification criteria.