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EC number: 202-945-6 | CAS number: 101-48-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 27 Feb - 03 Mar 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Version / remarks:
- 2008
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Staatliches Gewerbaufslchtsamt Hildeshelm
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: Control and highest test item concentration (100 mg/L)
- Sampling method: Samples were taken for analysis from the freshly prepared solutions at 0 and 48 hours, and the old solutions at 48 and 96 hours.
- Sample storage conditions before analysis: All original samples were stored at room temperature. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Appropriate volume of the test item was pipetted directly into the test vessel and filled with dilution water and mixed with an ultraturrax (1 min, 17000 rpm).
- Controls: yes, test medium control
- Eluate: no
- Differential loading: yes - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: zebrafish
- Age at study initiation (mean and range, SD): at least 12 days
- Length at study initiation (length definition, mean, range and SD): 2.86 cm
- Weight at study initiation (mean and range, SD): 0.27 g
- Method of breeding: at 23 ± 2 °C and diffuse light (7 - 750 Lux, natural photoperiod). The water was changed at least once per week. The dissolved oxygen concentration was more than 80% of the air saturation value.
ACCLIMATION
- Acclimation period: at least 12 days
- Acclimation conditions (same as test or not): same as test
- Type and amount of food during acclimation: The amount of food was 4% of the fish body weight per feeding day with Sera Vipan; Sera GmbH, Heinsberg, Germany
- Feeding frequency during acclimation: Food was provided 3 times per week. The test fish were not be fed 24 h before test start.
- Health during acclimation (any mortality observed): No mortality was observed during this time. No disease treatments were administered throughout holding and testing.
FEEDING DURING TEST: Fish were not fed during the test. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 71 mg/L as CaCO3
- Test temperature:
- 22.6 - 22.9 °C (Control)
22.5 - 23.0 °C (100 mg/L) - pH:
- 7.71 - 7.91 (Control)
7.45 - 7.94 (100 mg/L) - Dissolved oxygen:
- 92 - 100% (Control)
90 - 100% (100 mg/L) - Conductivity:
- 181 µS/cm
- Nominal and measured concentrations:
- Control and 100 mg/L (threshold approach)
- Details on test conditions:
- TEST SYSTEM
Test vessel:
- Type: open (covered with glass plates)
- Material, size, headspace, fill volume: Glass aquaria of 24 L, filled with approx 20 L of dilution water (dimensions: 22/39/28 cm, depth of water: 23.5 cm)
- Renewal rate of test solution (frequency/flow rate): after 48 h
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: Fish density in the test vessels was 0.094 g fish per litre test solution
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap water of local origin was used for holding; filtered on activated charcoal and aerated for at least 24 h to remove chlorine
- Total hardness: 10 - 250 mg CaCO3/L
- pH-value: 6.0 - 8.5
- Acidity: 0.2 mmol/L
- Acid capacity: 1.0 mmol/L
- Conductivity: 181 µS/cm
- Culture medium different from test medium: no
- Intervals of water quality measurement: The pH-value, temperature and oxygen saturation were measured in all test vessels at start and end of the exposure and every 24 h.
OTHER TEST CONDITIONS
- Photoperiod: 16 hours photoperiod
- Light intensity: 7 - 750 Lux
EFFECT PARAMETERS MEASURED: Mortality after 4, 24, 48, 72 and 96 h; fish were considered dead if there was no visible movement and if touching of the caudal peduncle produced no reaction. Records were kept of visible abnormalities.
- Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: slow escape reflex was observed from 24 to 72 h. At test end no escape reflex was obsreved for three fish and slow escape reflex for four fish.
- Mortality of control: no
- Others: Analytical Results are summarized with the Table 1 in section "Any other information on results incl tables". - Reported statistics and error estimates:
- The LC0 and LC100 after 96 h was derived from the raw data of the limit concentration. LC50-values do not have to be calculated in a limit test.
- Sublethal observations / clinical signs:
The analytical results are presented in the following table.
Table 1: Measured Concentrations and Percent of the Nominal Concentrations of the Test Item
Sampling date
2017-02-27 0 hours (Fresh medium)
2017-03-01 48 hours (Old medium)
2017-03-01 48 hours (Fresh medium)
2017-03-03 96 hours (Old medium)
Start of analysis
2017-02-27
2017-03-01
2017-03-01
2017-03-03
Phenylacetaldehyde Dimethyl Acetal
Nominal concentration of the test item [mg/L]
Meas. conc. [mg/L]
%
Meas. conc. [mg/L]
%
Meas. conc. [mg/L]
%
Meas. conc. [mg/L]
%
100
84.5 1)
84
92.4 1)
92
105 1)
105
89.4
89
Control
< LOQ
< LOQ
< LOQ
< LOQ
Meas. conc. = Measured concentration of the test item, dilution factor taken into account, mean value of two injections
% = Percent of nominal concentration of the test item
LOQ = Limit of Quantification (2.0 mg/L test item)
1) = reanalyzed on 2017-03-02
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test item had no acute toxic effects on Danio rerio in a 96-h static test according to OECD 203 and GLP up to 100 mg/L (nominal). No mortality was recorded and a LC50 (96 h) > 100 mg/L was derived.
Reference
Description of key information
LC50 (96 h): > 100 mg/L (OECD 203, Danio rerio, nominal)
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
One experimental study is available investigating the effects of the substance to freshwater fish. The study was performed according to OECD 203 (GLP) using Danio rerio as test organism under semi-static conditions. A threshold approach was performed with control and a limit concentration of 100 mg/L. The actual exposure concentrations were verified by LC-MS analysis after 0 and 48 hours and from the corresponding 48 hours aged test media after 48 and 96 h of the study (fresh and aged media). Since the measured concentrations were within the range between 80 and 120% of nominal, the effect concentrations were based on the nominal concentrations. After an exposure time of 96 h a LC50 of >100 mg/L resulted based on mortality.
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