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EC number: 701-065-4 | CAS number: -
- Life Cycle description
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- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
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- Water solubility
- Solubility in organic solvents / fat solubility
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- Endpoint summary
- Stability
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- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
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- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
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- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- It is considered appropriate to address the data requirements for synthetic amorphous magnesium silicate by read-across to the available studies on structurally similar substances.
Short-term toxicity studies on Daphnia are available on silicon dioxide, which due to sharing similar structural, physical and chemical properties to synthetic amorphous magnesium silicate, is considered appropriate for read-across purposes. - Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Specific details on test material used for the study:
- Silicon dioxide is being used as a read-across for synthetic amorphous magnesium silicate.
- Duration:
- 24 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks:
- 1/40 animals were immobile
- Remarks on result:
- other: Data from Hooftman (1992a) key study on silicon dioxide
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks:
- 19/80 animals were immobile = 24% (average of the 2 tests)
- Remarks on result:
- other: Data from Hooftman (1992b) supporting study on silicon dioxide
- Duration:
- 24 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks:
- 1/40 animals were immobile = 2.5%
- Remarks on result:
- other: Data from Hooftmann (1992) supporting study on silicon dioxide
- Details on results:
- Key data from short-term Daphnia toxicity studies on silicon dioxide are used for read-across to synthetic amorphous magnesium silicate.
- Conclusions:
- The acute toxicity of the target substance, synthetic amorphous magnesium silicate, to Daphnia was estimated to be low (24 h EL50 = >10000 mg/L), based on read-across studies testing silicon dioxide.
- Executive summary:
Acute toxicity studies on Daphnia magna are available for the read-across substance silicon dioxide. Due to sharing similar structural, physical and chemical properties to synthetic amorphous magnesium silicate, silicon dioxide is considered appropriate for read-across purposes.
The acute toxicity of silicon dioxide toD. mangawas shown to be low in all three studies used for read-across. The acute toxicity of the target substance, synthetic amorphous magnesium silicate, to Daphnia was therefore estimated to be low (24 h EL50 = > 10000 mg/L), based on read-across studies testing silicon dioxide.
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 12 Dec. 1991 - 13 Dec. 1991
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- 24 h instead of 48 h study
- Reason / purpose for cross-reference:
- reference to other study
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- yes
- Remarks:
- Test duration 24 h (acc. to the valid guideline of 04 April 1984) instead of 48 h (today) / In one test, the oxygen content was 4.2 mg/L after 24 h, i.e. less than 60 % of saturation (not assumed to have affected the outcome).
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: stirring for 20 h at 20 °C, and standing for 4 h before use
- Eluate: no, suspensions used after settling
- Differential loading: yes, limit tests with 1000 and 10,000 mg/L
-Evidence of undissolved material: The test media remained turbid throughout the test, and starchy particles
were observed on the bottom of the test vessels
Controls: Synthetic medium from groundwater - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: see above
- Strain: --
- Source: laboratory culture
- Age at study initiation (mean and range, SD): <24 h
- Method of breeding: Standard conditions according to principles of NPR 6503 (Nederlandse practijkrichtlijn of 1980)
Every week, new cultures started with approx. 125 daphniae of the same age (1 day old), fed on Chlorella (about 4 x10^9 cells and about 0.13 g yeast per 4 L
- Feeding during test: no - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 24 h
- Post exposure observation period:
- none
- Hardness:
- 204 mg/L as CaCO3
- Test temperature:
- 20 +-1 °C
- pH:
- 7.4 - 8.1
- Dissolved oxygen:
- 4.2 - 8.4 mg/L
- Salinity:
- not applicable
- Nominal and measured concentrations:
- 1000 and 10,000 mg SiO2/L (nominal, loading)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 150-mL beaker
- Type: open
- Material, size, headspace, fill volume: glass beaker, 50 mL headspace, 100 mL test volume
- Aeration: none
- Renewal rate of test solution (frequency/flow rate): none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 8
- No. of vessels per pure control (replicates): 8 (with synthetic medium)
- Biomass loading rate: 5 animals/ 100 mL
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: groundwater with mineral salts supplemented (synthetic medium)
- Total organic carbon: 1.7 mg/L
- Alkalinity: no data
- Ca/Mg ratio: 1.83
- Conductivity: no data
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light, 8 h dark
- Light intensity: no data - Reference substance (positive control):
- no
- Duration:
- 24 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks:
- : 1/40 animals were immobile = 2.5 %
- Details on results:
- - Mortality/immobility of control: 0/40
- Other adverse effects control: none
- Abnormal responses: Immobilisation effects can be attributed to physical hampering of the daphnias.
- Detailed results (see also Table under "Remarks on results..." below):
3/40 (15 %) and 1/40 (2.5 %) of the daphniae were immobile/dead at a loading of 1000 and 10000 mg/L, respectively
(non-filtered suspension after settling). - Results with reference substance (positive control):
- not applicable
- Reported statistics and error estimates:
- not applicable
- Conclusions:
- The observed effects were not dose related, and it is likely that they are caused by physical hampering of the test animals.
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 29 Aug. 1991 - 30 Aug. 1991 / 04 Sep. 1991 - 05 Sep. 1991
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- 24 h instead of 48 h study
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- yes
- Remarks:
- Test duration 24 h (acc. to the valid guideline of 04 April 1984) instead of 48 h (today)
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: stirring for 20 h at 20 °C
- Eluate: no, suspensions used:
1st test non-filtered, settling of 4 h before use (Report, p. 10)
2nd test grossly filtered through a wad of perlon wool (still suspension).
- Differential loading: yes, limit tests with 1000 and 10,000 mg/L
- Controls: Synthetic medium from groundwater
- Evidence of undissolved material (e.g. precipitate, surface film, etc): suspended test material,
part of it accumulated at the surface, more pronounced in the suspension of 1000 mg/L than of 10,000 mg/L (Report, p. 13). - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: see above
- Strain: --
- Source: laboratory culture
- Age at study initiation (mean and range, SD): <24 h
- Method of breeding: standard conditions according to principles of NPR 6503 (Nederlandse practijkrichtlijn of 1980)
- Feeding during test: no
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 24 h
- Post exposure observation period:
- none
- Hardness:
- 204 mg/L as CaCO3
- Test temperature:
- 20 +-1°C
- pH:
- Test 1: 7.2 - 8.1
Test 2: 7.3 - 8.2 - Dissolved oxygen:
- Test 1: 6.2 - 8.8 mg/L
Test 2: 6.6 - 8.6 mg/L - Salinity:
- not applicable
- Nominal and measured concentrations:
- 1st test: 1000 and 10,000 mg SiO2/L (nominal, loading, non-filtered)
2nd test: 1000 and 10,000 mg SiO2/L (nominal, loading, filtered through perlon wool) - Details on test conditions:
- TEST SYSTEM
- Test vessel: 150-mL beaker
- Type: open
- Material, size, headspace, fill volume: glass, 50 mL headspace, 100 mL test volume
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates):16 (2 tests à 8 vessels)
- No. of vessels per pure control (replicates): 16 (2 tests à 8 with synthetic medium)
- Biomass loading rate: 5 animals / 100 mL
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: groundwater with mineral salts supplemented (synthetic medium)
- Total organic carbon: 1.5 mg/L
- Alkalinity: no data
- Ca/Mg ratio: 1.76
- Conductivity: no data
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light, 8 h dark
- Light intensity: no data
- Reference substance (positive control):
- no
- Duration:
- 24 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks:
- : 19/80 animals were immobile = 24 % (average of the 2 tests)
- Details on results:
- - Mortality/Immobility of control: none 0/80 (Test 1 and 2)
- Abnormal responses: Based on these pre-tests with suspensions, it is suspected that the immobility observed,
particularly with the 10,000 mg/l suspensions, could be attributed to physical effects
(Report, Tables B1 - B4: First and second test).
- Detailed results (see also Table under "Remarks on results..." below)
Sample 1 (Test 1):
2/40 (5%) and 10/40 (25%) of the daphnias were immobile at loadings of 1000 mg/l and 10,000 mg/l, respectively, non-filtered.
Sample 2 (Test 2):
4/40 (10%) and 9/40 (22.5%) of the daphnias were immobile (or dead in one case) at loadings of 1000 mg/l and 10,000 mg/l, respectively,
filtered through perlon wool.
Overall, in the presence of SiO2 suspensions of 1000 and 10,000 mg/L, 6/80 (7.5 %) and 19/80 (~24 %) of the animals were found immobile
after 24 h of exposure [Report, Table B1 - B4 (first + second test)]. - Results with reference substance (positive control):
- not applicable
- Reported statistics and error estimates:
- not applicable
- Validity criteria fulfilled:
- yes
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 26 Sep. 1991 - 27 Sep. 1991
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- 24 h instead of 48 h study
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- yes
- Remarks:
- Test duration 24 h (acc. to the valid guideline of 04 April 1984) instead of 48 h (today) / In one test, the oxygen content was 4.4 mg/L at time 0, i.e. less than 60 % of saturation (not assumed to have affected the outcome).
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: stirring for 20 h at 20 °C, followed by gross filtration (perlon wool) and microfiltration (1.7 µm and/or 1.2 µm)
- Eluate: no, filtrate
Filtration: through perlon wool, then through glass-fibre filter
A filtrate through 1.7 microfilter (still milky/cloudy)
B. filtrate through 1.7 and 1.2 µm microfilter (visibly clear)
- Differential loading: no, limit test with 1000 mg/L
- Controls: Synthetic medium from groundwater
Note: In two pre-tests, suspensions with 1000 and 10,000 mg/L SiO2 were used.
(see following entry: 02_Degussa AG 92-0139-DGO_pre-test, short-term toxicity to aquatic invertebrates) - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: see above
- Strain: --
- Source: laboratory culture
- Age at study initiation (mean and range, SD): <24 h
- Method of breeding: standard conditions according to principles of NPR 6503 (Nederlandse practijkrichtlijn of 1980)
- Feeding during test: no
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 24 h
- Post exposure observation period:
- none
- Hardness:
- 204 mg/L as CaCO3
- Test temperature:
- 20 +-1°C
- pH:
- 7.9 - 8.2
- Dissolved oxygen:
- 4.4 - 8.3 mg/L
- Salinity:
- not applicable
- Nominal and measured concentrations:
- 1000 mg/L SiO2 nominal
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 150-ml beaker
- Type: open
- Material, size, headspace, fill volume: glass, 50 mL headspace, 100 mL test volume
- Aeration: none
- Renewal rate of test solution (frequency/flow rate): none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 8
- No. of vessels per pure control (replicates): 8 (synthetic medium)
- No. of vessels per filtration control (replicates): 8 (test in synthetic medium passed through perlon filter)
- Biomass loading rate: 5 animals/100 mL
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: groundwater with mineral salts supplemented (synthetic medium)
- Total organic carbon: 1.5 mg/L
- Alkalinity: no data
- Ca/mg ratio: 1.76
- Conductivity: no data
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light, 8 h dark
- Light intensity: no data
- Reference substance (positive control):
- no
- Duration:
- 24 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks:
- : 1/40 animals were immobile
- Duration:
- 24 h
- Dose descriptor:
- EL0
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks:
- : 1/40 animals were immobile
- Details on results:
- - Mortality/immobility of control: none [0/40 animals]
- Mortality/immobility of treated animals: 1/40
Overall, 1/40 animals treated with the WAFs was found immobile after 24 h of exposure (2.5 %) (Report, Table B5 and B6 [Third (main) test]:
This consisted of two parallel series using clear or slightly milky solutions of the water-soluble fractions (WSF):
0/15 immobile animals (0 %) (assumed to relate to test medium microfiltrated 1.7 µm #)
1/25 immobile animals (4 %) (in the clear solution, assumed to relate to test medium microfiltrated 1.7 µm and 1.2 µm #)
- Abnormal responses: Based on pre-tests with suspensions, it is suspected that the immobility observed,
particularly with the 10,000 mg/l suspensions, could be attributed to physical effects.
(see following entry 02_Degussa 92-0139-DGO_pre-test.)
-----------------------------------
# Note: The wording under 3.5 (p. 14) says that "....there was one immobile animal (out of 25) in the clear solution prepared by filtration of the 1000 mg/L solution". This statement would correlate with the test medium microfiltrated at 1.7 and 1.2 µm, according to Report 3.4.3 (p. 14). However, the allocation of the results to the filtration conditions is inconsistent due to contradictory indexing in the Tables B5 and B6 of the Report (p.24/25).
- Results with reference substance (positive control):
- not applicable
- Reported statistics and error estimates:
- not applicable
- Validity criteria fulfilled:
- yes
- Conclusions:
- Aerosil 200 is not acutely toxic at a loading of >=1 000 mg/l.
Referenceopen allclose all
Overview of results - Number of mobile animals at given loadings of Ultrasil VN 3
(from Report, Table C1)
Test |
|||
Time [h] |
Loading [mg/L] |
||
0 |
1000*) |
10,000*) |
|
0 |
40 |
40 |
40 |
24 |
40 |
37 |
39 |
*) Supernatant of the suspensions used after 4-h settling
Overview of the results - Number of mobile animals at given loadings of Aerosil 200
(from Report, Tables B1 and B3)
Test 1 |
Loading [mg/L] |
|||
Time [h] |
||||
0 |
-- |
1000*) |
10,000*) |
|
0 |
40 |
-- |
40 |
40 |
24 |
40 |
-- |
38 |
30 |
Test 2 |
Loading [mg/L] |
|||
Time [h] |
||||
0 |
0**) |
1000**) |
10,000**) |
|
0 |
40 |
40 |
40 |
40 |
24 |
40 |
40 |
36 |
31 |
*) non-filtered suspension
**)previously filtered through perlon wool
Number of mobile animals at given loadings of Aerosil 200
(from Report, Table B5):
Test 3 |
Loading [mg/L] |
|||
Time [h] |
||||
0 |
0*) |
1000*) |
1000**) |
|
0 |
40 |
40 |
25 |
15 |
24 |
40 |
40 |
24 |
15 |
*) filtered through perlon wool, followed by microfiltration of 1.7- and 1.2-µm pore size
**) filtered through perlon wool, followed by microfiltration of 1.7-µm pore size
Description of key information
No acute toxicity data on daphnia for magnesium silicate available, however, based on read-across from silicon dioxide it is expected to shows no acute toxicity with an EL50 >10,000 mg/L (effective loading).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 10 000 mg/L
Additional information
Based on the high tolerance and ubiquitous natural occurrence of this substance class, the need for generation of data for this specific compound is waived in line with REACH Regulation 1907/2006, Annex IX, Section 9.1.
There was no adverse effect on daphniae in the presence of water-accommodated fractions ofstructure-analogous synthetic amorphous silica (SAS). Therefore, it is concluded that magnesium silicate will
also not be toxic to daphnia.
It is assumed that the freshwater results are also applicable to marine life, i.e. no acute aquatic toxicity expected from exposure to magnesium silicate.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
