Poly(polyalkylammonium) poly(µ-alkoxy)-µ6-oxido-polyhedropoly(chloridometal)ate polyalkanol solvate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study according to OECD and GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2013

Report date:

2013

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Indate (2-), hexachlorododeca-µ-methoxy-µ6-oxohexa-, hydrogen, compd. with N-methylmethanamine (1:2:2)

Cas Number:

1381802-10-3

Molecular formula:

[In6O(OMe)12Cl6]2–[NH2Me2]+2 · 2MeOH

IUPAC Name:

Indate (2-), hexachlorododeca-µ-methoxy-µ6-oxohexa-, hydrogen, compd. with N-methylmethanamine (1:2:2)

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

Batch number V107 – V110
Purity > 99 %
Molecular weight 1446.302 g/mol
Appearance Colorless, crystalline powder
Water solubility Reacts (sensitive to hydrolysis, t ½ (pH 7): approx. 5 minutes

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

The tested limit concentration of 100 mg/L of the test item iXsenic S Gen 3.4 and the control were analytically verified at the start of the exposure (0 h) and at the end of the exposure (48 h).

Test solutions

Vehicle:

no

Details on test solutions:

A stock solution (100 mg/L of the test item were weighed out) was freshly prepared with dilution water.
The test item dispersion was stirred with a magnetic stirrer (approx. 1100 rpm) at room temperature for 2 h. Since the test item is known to hydrolyse quickly (t1/2 pH 7: approx. 5 min), the reaction products were tested.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna STRAUS (Clone 5) was used as test organisms.
20 daphnids, divided into 4 replicates, each with 5 daphnids per limit concentration and control were tested. 2 to 24 h old daphnids were used for the study. They were obtained by removing the parental daphnids twice within 22 h. Daphnids were left for acclimatisaion for at least 2 h in dilution water.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Test temperature:

During the test the incubator temperature was 19 – 20 °C.

pH:

Nominal
test item
concentration pH-value
[mg/L] 0h 48h (replicate 1-4)
100 7.10 7.69 7.63 7.60 7.56
Control 7.75 7.57 7.69 7.71 7.70

Dissolved oxygen:

Nominal
test item
concentration Dissolved O2-concentration [mg/L]
[mg/L] 0h 48h (replicate 1-4)
100 7.90 8.49 8.46 8.49 8.51
Control 8.17 7.91 8.19 8.16 8.23

Nominal and measured concentrations:

Measured TOC Concentrations in the Definitive Test

Sampling date 2013-06-05 2013-06-07
Start of the exposure, 0 End of the exposure, 48 h
Start of analysis 2013-06-05 2013-06-07
Nominal
test item
concentration
[mg/L] Total Organic Carbon (TOC)*
Measured concentration#[mg/L] Measured concentration# [mg/L] %
100 15.2 16.2 107
Control < 2.00 < 2.00 —

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

EC50: 1.66 mg/L (CI 1.48 - 2.01 mg/L)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

At the limit concentration of 100 mg/L of the test item, no effects were observed on Daphnia magna.

Executive summary:

In the acute immobilisation test with Daphnia magna (STRAUS), the effects of the limit concentration of 100 mg/L of the test item were determined according to OECD 202 (2004) and Council Regulation (EC) No. 440/2008 Method C.2 (2008). The limit test was conducted under static conditions over a period of 48 hours.