2-Propenoic acid, 2-methyl-, 7-oxabicyclo[4.1.0]hept-3-ylmethyl ester

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study and GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2014

Report date:

2014

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2-Propenoic acid, 2-methyl-, 7-oxabicyclo[4.1.0]hept-3-ylmethyl ester

IUPAC Name:

2-Propenoic acid, 2-methyl-, 7-oxabicyclo[4.1.0]hept-3-ylmethyl ester

Details on test material:

CAS No. 82428-30-6
Appearance slight yellow liquid
Water solubility 3.35 g/L (20°C)
Molecular weight 196.25 g/mol
Formula C11H16O3
Density 1.079 g/mL at 20°C
Recommended storage Cool, protected from light

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

83.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: (CI: 63.2 - 105)

Any other information on results incl. tables

Effect levels	Test duration [h]	Nominal test item concentrations [mg/L]
EC10 (with 95 % confidence limits)	24	176	(Cl: 52.3 – > 200)
	48	11.0	(Cl: 7.26 – 19.0)
EC50 (with 95 % confidence limits)	24	192	(Cl: 56.6 – > 200)
	48	83.4	(Cl: 63.2 – 105)
EC100	24	> 200
	48	200
NOEC	48	12.5
LOEC	48	25.0

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Based on the nominal concentrations of the test item S-100; 3,4-Epoxycyclohexylmethyl methacrylate, the 48 h-EC50 for Daphnia magna was 83.4 mg/L (95 % confidence limits: 63.2 – 105 mg/L).
The NOEC after 48 h was 12.5 mg/L. The LOEC after 48 h was 25.0 mg/L.

Executive summary:

In the acute immobilisation test with Daphnia magna (STRAUS), the effects of the test item S-100; 3,4-Epoxycyclohexylmethyl methacrylate (batch number:12051006) were determined according to OECD 202 (2004) from 2014-10-21 to 2014-10-23 at Dr.U.Noack-Laboratorien, Käthe-Paulus-Str. 1, 31157 Sarstedt, Germany.

The study was conducted under semi-static conditions over a period of 48 h with 6 concentrations of the test item S-100; 3,4-Epoxycyclohexylmethyl methacrylatein the range of 6.25 - 200 mg/L, prepared with dilution water in a geometric series with a separation factor of 2.

The test item S-100; 3,4-Epoxycyclohexylmethyl methacrylateis a clear, pale yellow liquid with a water solubility of 3.35 g/L (20 °C).The tested solutions were clear throughout the exposure period.

Twenty daphnids were exposed to each concentration leveland the control.

The concentrations of the test item S-100; 3,4-Epoxycyclohexylmethyl methacrylate were analytically verified via HPLC-DAD at the start of the exposure intervals (0 and 24 h) and at the end of the exposure intervals (24 and 48 h) in all concentration levels and the control.

The measured concentrations of the test item at the start of the exposure intervals (0 and 24 h) were in the range of 98 to 101 % of the nominal values. At the end of the exposure intervals (24 and 48 h), the measured concentrations of the test item were in the range of 97 to 99 % of the nominal values.

The measured test item concentrations were all within ±20 % of the nominal concentrations. This indicates that the test item concentrations were successfully maintained for the duration of the test. Therefore, the effect levels given are based on the nominal concentrations of the test item S-100; 3,4-Epoxycyclohexylmethyl methacrylate.

The water quality parameters (i.e. pH-value and dissolved oxygen concentration), measured at the start (0 and 24 h) and at the end of the exposure intervals (24 and 48 h), were within the acceptable limits. The validity criteria of the test guideline were fulfilled.