Rape oil, reaction products with diethylenetriamine

Partition coefficient

Link to relevant study record(s)

Reference

Endpoint:

partition coefficient

Type of information:

experimental study

Adequacy of study:

key study

Study period:

08 April 2016 to 09 May 2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 117 (Partition Coefficient (n-octanol / water), HPLC Method)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method A.8 (Partition Coefficient - HPLC Method)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

HPLC method

Partition coefficient type:

octanol-water

Analytical method:

high-performance liquid chromatography

Key result

Type:

log Pow

Partition coefficient:

> 10

Temp.:

40 °C

pH:

7.1

Remarks on result:

other: Log Pow 5.86 to > 10.0

Remarks:

98 % of test item determined to have a partition coefficient > 1.0 x E10 (Log Pow 10.0)

Details on results:

RESULTS
- The estimated Log10 Pow for the representative structures of the test item were calculated to be 6.40 to > 10.0.
- Typical chromatography for the definitive test is presented in Appendix 2 (attached).
- Retention times of the dead time and retention times, capacity factors (k’) and Log10 Pow values for the reference standards are shown in Table 13 (attached).
- The calibration curve from which the sample results were taken is shown in Figure 6 (attached).
- Retention times, capacity factor and Log10 Pow values determined for the sample are shown in Table 14 (attached).

Conclusions:

The partition coefficient of the test item was determined to be in the range 7.20 x 10E05 to > 1.0 x 10E10 with a Log10 Pow in the range 5.86 to > 10.0. By area percentage normalisation, approximately 98 % of the test item had a partition coefficient > 1.0 x 10E10 (Log Pow > 10.0).

Executive summary:

GUIDELINE

The determination was carried out using the HPLC method and a procedure designed to be compatible with Method A.8 Partition Coefficient of Commission Regulation (EC) No 440/2008 of 30 May 2008 and Method 117 of the OECD Guidelines for Testing of Chemicals, 13 April 2004.

 

RESULTS

The partition coefficient of the test item was determined to be in the range 7.20 x 10E05 to > 1.0 x 10E10 with a Log10 Pow in the range 5.86 to > 10.0. By area percentage normalisation, approximately 98 % of the test item had a partition coefficient > 1.0 x 10E10 (Log Pow > 10.0).

Description of key information

The partition coefficient of the test item was determined to be in the range 7.20 x 10E05 to > 1.0 x 10E10 with a Log10 Pow in the range 5.86 to > 10.0. By area percentage normalisation, approximately 98 % of the test item had a partition coefficient > 1.0 x 10E10 with Log Pow > 10.0 (OECD 117 and EU Method A.8).

Key value for chemical safety assessment

Log Kow (Log Pow):

8

at the temperature of:

40 °C

Additional information

GUIDELINE

The determination was carried out using the HPLC method and a procedure designed to be compatible with Method A.8 Partition Coefficient of Commission Regulation (EC) No 440/2008 of 30 May 2008 and Method 117 of the OECD Guidelines for Testing of Chemicals, 13 April 2004.

 

RESULTS

The partition coefficient of the test item was determined to be in the range 7.20 x 10E05 to > 1.0 x 10E10 with a Log10 Pow in the range 5.86 to > 10.0. By area percentage normalisation, approximately 98 % of the test item had a partition coefficient > 1.0 x 10E10 (Log Pow > 10.0).

EXPOSURE / RISK ASSESSMENT

No evidence of short-term aquatic toxicity was observed at loading rates in excess of water solubility for three trophic levels and results were reported as LL50 > 100 mg/L (fish), EL50 > 100 mg/L (Daphnia) and EL50 > 100 mg/L (algae). The NOEC was therefore 100 mg/L loading rate WAF for fish, Daphnia and algae. In the absence of demonstrated aquatic toxicity, the appropriate overall conclusion is no hazard identified (see ECHA Guidance on Information Requirements and Chemical Safety Assessment Part D: Framework for exposure assessment (Version 2.0; August 2016). Nevertheless, it is required that environmental exposure is shown to be not significant by demonstrating predicted exposure is always well below the relevant PNEC when a detailed lifecycle is assessed (see REACH Annex XI Section 3.2(a) and ECHA Guidance on information requirements and chemical safety assessment Chapter R.5: Adaptation of information requirements; Version 2.1; December 2011). Since the experimentally determined Log Pow value of > 10.0 lies outside the boundaries of the EUSES model, the upper limit of 8.0 assumed by that model was used to allow correct assessment of secondary poisoning. The Predicted Effect Concentration (PEC) values were equivalent or higher in each case using this strategy and therefore represent the worst case with respect to environmental exposure.