Tetrachloro-p-benzoquinone

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well documented study report performed before OECD and GLP guideline implementation. Important aspects in line with current OECD guidelines.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1975

Report date:

1975

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

not specified

GLP compliance:

no

Remarks:

prior to GLP implementation

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Tetrachloro-p-benzoquinone

EC Number:

204-274-4

EC Name:

Tetrachloro-p-benzoquinone

Cas Number:

118-75-2

Molecular formula:

C6Cl4O2

IUPAC Name:

2,3,5,6-tetrachloro-1,4-benzoquinone

Details on test material:

- Name of test material (as cited in study report): Chloranil (2,3,5,6-tetrachlorbenzochinon-1,4)

Test animals

Species:

rat

Strain:

Wistar

Sex:

female

Details on test animals or test system and environmental conditions:

Female rats were used, as no sex linked differences in sensitivity were seen in preliminary tests. Body weight at treatment = 90 - 132 g, average weight = 107 g. Commercially available rat diet and tap water were fed ad libitum, except during the fasting period prior to and after administration, when food was withdrawn from 16 hours before until 2 hours post administration.

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other: sesame oil

Details on oral exposure:

The test substance was suspended in the vehicle, sesame oil. The test substance was administered as a 25% prepartaion in the vehicle.

Doses:

2500, 4000, 6300, 10000, 15000 mg/kg bw

No. of animals per sex per dose:

10 females

Control animals:

no

Details on study design:

Post-dosing observation period was 14 days during which mortality was recorded. Body weight was recorded at study start (day of dosing) for each animal and 7 and 14 days after application for each survivor. Premature deaths and survivors killed at the end of the 14-day post-dosing observation period were necropsied and macroscopic findings recorded.

Statistics:

Probit analysis

Results and discussion

Effect levels

Sex:

female

Dose descriptor:

LD50

Effect level:

6 951 mg/kg bw

Based on:

test mat.

Remarks on result:

other: Only females tested, as there were no indications of sex linked differences in sensitivity in pretests.

Mortality:

2500 mg/kg: 0/10 (f)
4000 mg/kg: 0/10 (f)
6300 mg/kg: 4/10 (f)
10000 mg/kg: 9/10 (f)
15000 mg/kg: 10/10 (f)

Mortality occurred within 1/2 to 8/9 days post treatment.

Clinical signs:

other: The general condition of decedent animals became increasingly worse prior to death.

Gross pathology:

Necropsy of dead and finally killed animals did not reveal any overt signs of toxicity.

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: expert judgment

Conclusions:

The oral LD50 in female rats was found to be 6951 mg/kg bw.

Executive summary:

2,3,5,6-tetrachloro-1,4-benzoquinone was tested for its acute toxicity by oral gavage administration in female rats. Only female rats were included in this study, as there was no indication of sex linked differences in sensitivity in preliminary tests. An LD50 of 6951 mg/kg bw was calculated. At a single dose of 4000 mg/kg and at all lower doses administered, all animals survived the 14-day post treatment observation period and body weights were not adversely affected. The higher doses of 6300, 10000 and 15000 mg/kg induced a dose-related increase in mortality (40%, 90% and 100%, respectively) with transient bodyweight loss in one female survivor each at 6300 mg/kg and at 10000 mg/kg, and body weight loss over 7 days post treatment followed by premature death in one female treated with 15000 mg/kg. In all other survivors of this study bodyweight was not adversely affected by treatment. The general condition of decedent animals became increasingly worse prior to death. Necropsy did not reveal any overt signs of toxicity in premature deaths or animals which survived until the scheduled necropsy day.