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Diss Factsheets

Toxicological information

Skin sensitisation

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Administrative data

Endpoint:
skin sensitisation: in vivo (non-LLNA)
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Justification for type of information:
Data is from publication.

Data source

Reference
Reference Type:
publication
Title:
CONTACT ECZEMA CAUSED BY TRUE TEAK (Tectona grandis)
Author:
H. K. KROGH
Year:
1964
Bibliographic source:
BRITISH JOURNAL OF INDUSTRIAL MEDICINE, 1964, 21, 65

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: As mention below
Principles of method if other than guideline:
To evaluate the skin sensitizing potential of 2-methyl-9,10-anthraquinone in furniture factory workers by patch test.
GLP compliance:
not specified
Type of study:
patch test
Justification for non-LLNA method:
Not specified.

Test material

Constituent 1
Chemical structure
Reference substance name:
2-methylanthraquinone
EC Number:
201-539-6
EC Name:
2-methylanthraquinone
Cas Number:
84-54-8
Molecular formula:
C15H10O2
IUPAC Name:
2-methyl-9,10-anthraquinone
Details on test material:
Name of test material (as cited in study report): Tectoquinone (synonym for target chemical)
Molecular formula (if other than submission substance): C15H10O2
- Molecular weight (if other than submission substance): 222.24 g/mol
Substance type: Organic
Physical state: Solid
Specific details on test material used for the study:
-Name of test material (as cited in study report): 2-methylanthraquinone /tectoquinone (2-methyl-9,10-anthraquinone)
- Molecular formula: C15H10O2
- Molecular weight: 222.242 g/mol
- Smiles notation: Cc1ccc2c(c1)C(=O)c3ccccc3C2=O
- Substance type: Organic
- Physical state: Solid

In vivo test system

Test animals

Species:
other: Human
Strain:
other:
Remarks:
Not applicable
Sex:
not specified
Details on test animals and environmental conditions:
Details on test animal
TEST ANIMALS
72 furniture factory workers were investigated with the history of allergic contact eczema caused by true teak (Tectona grandis). All the 72 workers were interviewed and examined for skin lesions.

Study design: in vivo (non-LLNA)

Induction
Route:
epicutaneous, occlusive
Vehicle:
not specified
Concentration / amount:
Native teak dust was applied which contain unknown concentration of 2-methyl-9,10-anthraquinone.
Day(s)/duration:
24 hour
Challenge
Route:
epicutaneous, occlusive
Vehicle:
other: Acetone
Concentration / amount:
0.1,0.5, 1 and 2%
Day(s)/duration:
24 hour
No. of animals per dose:
72 Human
Details on study design:
Details on study design
MAIN STUDY
A. INDUCTION EXPOSURE
- No. of exposures: 1
- Exposure period: 24 hour
- Test groups: 72
- Control group:10
- Site: The patch was applied on the volar side of the left forearm.
- Frequency of applications: No data available.
- Duration: 24 hour
- Concentrations: native teak dust was applied which contain unknown concentration of 2-methyl-9,10-anthraquinone.

B. CHALLENGE EXPOSURE
- No. of exposures:4
- Day(s) of challenge: No data available.
- Exposure period: 24 hour
- Test groups:72
- Control group:10
- Site: The patch was applied on the volar side of the left forearm.
- Concentrations: 0.1,0.5, 1 and 2%
- Evaluation (hr after challenge):24 ,48 and 72 hour

Other-The interpretation of the patch tests
was based on the usual criteria and was recorded as allergic, toxic, or negative.
Challenge controls:
Yes, 10 challenge controls were investigated
Positive control substance(s):
not specified

Study design: in vivo (LLNA)

Statistics:
No data available.

Results and discussion

Positive control results:
No data available.

In vivo (non-LLNA)

Resultsopen allclose all
Reading:
other: challenge
Hours after challenge:
24
Group:
test chemical
Dose level:
0.1,0.5, 1 and 2%
No. with + reactions:
0
Total no. in group:
72
Clinical observations:
No skin sensitization effect were observed.
Remarks on result:
no indication of skin sensitisation
Reading:
other: challenge
Hours after challenge:
48
Group:
test chemical
Dose level:
0.1,0.5, 1 and 2%
No. with + reactions:
0
Total no. in group:
72
Clinical observations:
No skin sensitization effect were observed.
Remarks on result:
no indication of skin sensitisation
Reading:
other: challenge
Hours after challenge:
72
Group:
test chemical
Dose level:
0.1,0.5, 1 and 2%
No. with + reactions:
0
Total no. in group:
72
Clinical observations:
No skin sensitization effect were observed.
Remarks on result:
no indication of skin sensitisation

In vivo (LLNA)

Cellular proliferation data / Observations:
CLINICAL OBSERVATIONS: No toxic effect were observed.

Applicant's summary and conclusion

Interpretation of results:
other: not sensitizing
Conclusions:
2-methyl-9,10-anthraquinone(84-54-8) was assessed for its possible contact allergenic potential in wood factory workers. 2-methyl-9,10-anthraquinone was considered to be negative in human by Patch test .
Executive summary:

2-methyl-9,10-anthraquinone(84-54-8) was assessed for its possible contact allergenic potential in wood factory workers by Patch test. A group of 72 furniture factory workers were investigated with the history of allergic contact eczema caused by true teak (Tectona grandis). All the 72 workers were interviewed and examined for skin lesions. For this purpose a Patch test was performed with native teak dust. The patch were applied for 24 hours on the volar side of the left forearm and read at the end of this period and read again after 48 and 72 hours. A challenge exposure was performed on the workers who showed allergic reactions. The patch was applied at the concentration of 0.1, 0.5, 1 and 2% tectoquinone. Acetone was used as vehicle.The interpretation of the patch tests was based on the usual criteria and was recorded as allergic, toxic, or negative.Identical Patch tests were applied on 10 controls. No skin sensitization effects were observed in test group or control group. Therefore2-methyl-9,10-anthraquinone (84-54-8) was considered to be non sensitizing in human by Patch test.