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Registration Dossier
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EC number: 240-093-7 | CAS number: 15958-68-6
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- data is from peer reviewed journals
Data source
Reference
- Reference Type:
- publication
- Title:
- THE OCULAR EFFECT OF SEVERAL IRRITANT DRUGS APPLIED DIRECTLY TO THE CONJUNCTIVA
- Author:
- JOSE J. ESTABLE
- Year:
- 1 948
- Bibliographic source:
- American Journal of Ophthalmology; Volume 31, Issue 7, July 1948, Pages 837-844
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- To compare the immediate and delayed effects of 9,10–anthraquinone (CAS No: 84-65-1) on the cornea—a tissue normally devoid of blood vessels with their effects on the vascular tissues of the sclera and eyelids
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Anthraquinone
- EC Number:
- 201-549-0
- EC Name:
- Anthraquinone
- Cas Number:
- 84-65-1
- IUPAC Name:
- 9,10-anthraquinone
- Test material form:
- solid
- Details on test material:
- Name of the test chemical: 9,10–anthraquinone
Molecular Formula: C14H8O2
Molecular Weight:208.215 g/mol
Substance Type: Organic
Physical State: Solid
Constituent 1
- Specific details on test material used for the study:
- Name of the test chemical: 9,10–anthraquinone
Molecular Formula: C14H8O2
Molecular Weight:208.215 g/mol
Substance Type: Organic
Physical State: Solid
Test animals / tissue source
- Species:
- rabbit
- Strain:
- other: White rabbits
- Details on test animals or tissues and environmental conditions:
- no data available
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- yes
- Amount / concentration applied:
- 100% undiluted powder
- Duration of treatment / exposure:
- one-half minute.
- Observation period (in vivo):
- one day to several days
- Duration of post- treatment incubation (in vitro):
- no data available
- Number of animals or in vitro replicates:
- no data available
- Details on study design:
- TEST SITE
Area of exposure: lower conjunctival sac of one eye.
SCORING SYSTEM: The eyes were examined for evidence of inflammation, and changes in the corneal structures were observed with the bio-microscope
Results and discussion
In vitro
- Other effects / acceptance of results:
- no data available
In vivo
Results
- Irritation parameter:
- overall irritation score
- Basis:
- mean
- Time point:
- other: not specified
- Score:
- 0
- Reversibility:
- fully reversible within: few hours
- Remarks on result:
- no indication of irritation
- Irritant / corrosive response data:
- Anthraquinone produced only immediate sensory and inflammatory reaction (discomfort, blepharospasm, and conjunctival congestion) that disappeared very soon. The eyes appeared normal in a few hours.
Applicant's summary and conclusion
- Interpretation of results:
- other: not irritating
- Conclusions:
- Anthraquinone (CAS No: 84-65-1) produced only immediate sensory and inflammatory reaction (discomfort, blepharospasm, and conjunctival congestion) that disappeared very soon. The eyes appeared normal in a few hours.
Hence, it was concluded the irritation effects were due to mechanical action of the powder rather than toxic effects of anthraquinone. Therefore, Anthraquinone (CAS No: 84-65-1) can be considered not irritating to eyes. - Executive summary:
An eye irritation study was performed to compare the immediate and delayed effects of 9,10–anthraquinone (CAS No: 84-65-1) on the cornea—a tissue normally devoid of blood vessels with their effects on the vascular tissues of the sclera and eyelids.
White rabbits were used for the study. Anthraquinone(dry powder)was placed in the lower conjunctival sac of one eye and held there usually for one-half minute. This was repeated at intervals of one or several days.The other eye was treated with 0.7% solution of Sodium chloride and used as control.The eyes were examined for evidence of inflammation, and changes in the corneal structures were observed with the bio-microscope.
Anthraquinone produced only immediate sensory and inflammatory reaction (discomfort, blepharospasm, and conjunctival congestion) that disappeared very soon. The eyes appeared normal in a few hours. Hence, it was concluded the irritation effects were due to mechanical action of the powder rather than toxic effects of anthraquinone. Therefore, Anthraquinone (CAS No: 84-65-1) can be considered not irritating to eyes.
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