1,5-bis(cyclohexylamino)anthraquinone

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

secondary literature

Justification for type of information:

data is from NTRL reports

Data source

Reference

Reference Type:

secondary source

Title:

LETTER CONCERNING FIVE ENCLOSED STUDIES ON 2-ETHYLANTHRAQUINONE WITH ATTACHMENTS (SANITIZED)

Author:

NTRL Report

Year:

1991

Bibliographic source:

NTRL Report: OTS0534314; last updated 1991

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

The skin irritation potential of 2-ethylanthraquinone (CAS No: 84-51-5)was evaluated in albino rabbits according to the Draize method

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

2-ethylanthraquinone

EC Number:

201-535-4

EC Name:

2-ethylanthraquinone

Cas Number:

84-51-5

Molecular formula:

C16H12O2

IUPAC Name:

2-ethylanthraquinone

Test material form:

solid

Details on test material:

Name of the test chemical: 2-ethylanthraquinone
Molecular Formula: C16H12O2
Molecular Weight: 236.316 g/mol
SMILES: c12c(C(c3ccccc3C1=O)=O)ccc(c2)CC
InChI: 1S/C16H12O2/c1-2-10-7-8-13-14(9-10)16(18)12-6-4-3-5-11(12)15(13)17/h3-9H,2H2,1H3
Substance type: Organic
Physical State: Solid

Specific details on test material used for the study:

Name of the test chemical: 2-ethylanthraquinone
Molecular Formula: C16H12O2
Molecular Weight: 236.316 g/mol
SMILES: c12c(C(c3ccccc3C1=O)=O)ccc(c2)CC
InChI: 1S/C16H12O2/c1-2-10-7-8-13-14(9-10)16(18)12-6-4-3-5-11(12)15(13)17/h3-9H,2H2,1H3
Substance type: Organic
Physical State: Solid

Test animals

Species:

rabbit

Strain:

New Zealand White

Details on test animals or test system and environmental conditions:

Test Animals:
- Sex: male and female

Test system

Type of coverage:

occlusive

Preparation of test site:

other: intact and abraded

Vehicle:

unchanged (no vehicle)

Controls:

not specified

Amount / concentration applied:

0.5 ml (0.5g)

Duration of treatment / exposure:

24 hours

Observation period:

24 and 72 hours

Number of animals:

6 (3 male, 3 female)

Details on study design:

TEST SITE
- Area of exposure: trunk,
- % coverage:1 inch *1 inch
- Type of wrap if used: an impermeable occlusive wrapping

REMOVAL OF TEST SUBSTANCE
- Washing (if done):no
- Time after start of exposure:

SCORING SYSTEM: The sites were individually examined and scored separately for erythema and edema at 24 and 72 hours. The mean scores for 24 and 72 hours grading were averaged to determine final irritation indices.

Results and discussion

In vitro

Other effects / acceptance of results:

no data available

In vivo

Results

Irritation parameter:

primary dermal irritation index (PDII)

Basis:

mean

Time point:

other: average of 24 and 72 hours

Score:

0.15

Max. score:

8

Reversibility:

not specified

Remarks on result:

no indication of irritation

Irritant / corrosive response data:

The Primary Irritation Index after 24, 72 hours for 2-ethylanthraquione was 0.15.

Any other information on results incl. tables

Table 3: Primary Skin Irritation – Rabbits

Summary of Scores for skin irritation

Rabbit number	Skin condition	Hours
		24		72
		E^1	Ed^1	E^1	Ed^1
1	NA^2	0	0	0	0
	A^3	0	0	0	0
2	NA	0	0	0	0
	A	0	0	0	0
3	NA	1	0	0	0
	A	1	0	0	0
4	NA	0	0	0	0
	A	0	0	0	0
5	NA	0	0	0	0
	A	0	0	0	0
6	NA	1	0	0	0
	A	1	0	0	0
Average	NA	0.3	0	0	0
	A	0.3	0	0	0

Combined Averages = 0.6           

Primary Irritation Index = 0.15

Where,

1- E/Ed = Erythema and Edema 

2- NA = Non- abraded skin

3- A = Abraded skin

Applicant's summary and conclusion

Interpretation of results:

other: not irritating

Conclusions:

The Primary Irritation Index after 24, 72 hours for 2-ethylanthraquinone was 0.15.
Based on the score and scale of interpretation of PII scores, 2-Ethylanthraquinone (CAS No: 84-51-5) can be considered not irritating to skin.

Executive summary:

The skin irritation potential of 2-ethylanthraquinone (CAS No: 84-51-5) was evaluated in albino rabbits according to the Draize method. 6 albino New Zealand rabbits (3/sex) were used for the study. 0.5 ml (0.5 g) of the test material was applied to clipped areas of intact and abraded skin.

The abrasions were longitudinal epidermal incisions sufficiently deep to penetrate the stratum corneum, but not so deep as to destroy the integrity of the derma. Applications were made under occlusive patches (1" x 1" gauze, covered by adhesive tape). Following application of 2-ethylanthraquinone the entire trunk of each animal was covered with an impermeable occlusive wrapping. The animals were then immobilized. The wrapping and test material were removed 24 hours following application. The sites were individually examined and scored separately for erythema and edema at 24 and 72 hours. The mean scores for 24 and 72 hours grading were averaged to determine final irritation indices.

 The Primary Irritation Index after 24, 72 hours for 2-ethylanthraquinone was 0.15. Based on the score and scale of interpretation of PII scores, 2-Ethylanthraquinone (CAS No: 84-51-5) can be considered not irritating to skin.