1,5-bis(cyclohexylamino)anthraquinone

Endpoint summary

Description of key information

Skin irritation:

The dermal irritation potential of 1,5-bis(cyclohexylamino)-9,10-anthraquinone (CAS No: 15958-68-6)was estimated using OECD QSAR toolbox version 3.3 with logPow as the primary descriptor. The substance 1,5-bis(cyclohexylamino)-9,10-anthraquinone (CAS No: 15958-68-6)was estimated to be not irritating to the skin of New Zealand White rabbits. Based on the estimated result 1,5-bis(cyclohexylamino)-9,10-anthraquinone (CAS No: 15958-68-6)can be considered as non irritant to skin and can be classified under the category ˋ Not Classified’ as per CLP regulation.

 

Eye irritation:

The ocular irritation potential of 1,5-bis(cyclohexylamino)-9,10-anthraquinone (CAS No: 15958-68-6)was estimated using OECD QSAR toolbox version 3.3 with logPow as the primary descriptor. The substance 1,5-bis(cyclohexylamino)-9,10-anthraquinone (CAS No: 15958-68-6)was estimated to be not irritating into the eyes of New Zealand White rabbits. Based on the estimated result 1,5-bis(cyclohexylamino)-9,10-anthraquinone (CAS No: 15958-68-6)can be considered to be not irritating to eye and can be classified under the category ˋ Not Classified’ as per CLP regulation.

Key value for chemical safety assessment

Skin irritation / corrosion

Link to relevant study records

Reference

Endpoint:

skin irritation: in vivo

Type of information:

(Q)SAR

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

results derived from a valid (Q)SAR model and falling into its applicability domain, with limited documentation / justification

Justification for type of information:

Data is from OECD QSAR toolbox version 3.3 and QMRF report has been attached

Qualifier:

according to guideline

Guideline:

other: estimated data

Principles of method if other than guideline:

Prediction is done using QSAR Toolbox version 3.3

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material: 1,5-bis(cyclohexylamino)-9,10-dihydroanthracene-9,10-dione
- Molecular formula: C26H30N2O2
- Molecular weight: 402.535 g/mol
- Smiles notation: O=C1c2c(c(NC3CCCCC3)ccc2)C(=O)c2cccc(NC3CCCCC3)c12
- InChl: 1S/C26H30N2O2/c29-25-20-14-8-16-22(28-18-11-5-2-6-12-18)24(20)26(30)19-13-7-15-21(23(19)25)27-17-9-3-1-4-10-17/h7-8,13-18,27-28H,1-6,9-12H2
- Substance type: Organic
- Physical state: Solid

Species:

rabbit

Strain:

New Zealand White

Details on test animals or test system and environmental conditions:

No data available

Type of coverage:

semiocclusive

Preparation of test site:

clipped

Vehicle:

not specified

Controls:

not specified

Amount / concentration applied:

No data available

Duration of treatment / exposure:

4 hours

Observation period:

7 days

Number of animals:

No data available

Details on study design:

No data available

Irritation parameter:

overall irritation score

Basis:

mean

Time point:

7 d

Score:

0

Reversibility:

not specified

Remarks on result:

no indication of irritation

Irritant / corrosive response data:

No skin irritation was observed.

Estimation method: Takes mode value from the 5 nearest neighbours
Domain  logical expression:Result: In Domain

((((((("a" or "b" or "c" )  and ("d" and ( not "e") )  )  and ("f" and ( not "g") )  )  and ("h" and ( not "i") )  )  and "j" )  and "k" )  and ("l" and "m" )  )

Domain logical expression index: "a"

Referential boundary: The target chemical should be classified as AN2 AND AN2 >>  Michael-type addition, quinoid structures AND AN2 >>  Michael-type addition, quinoid structures >> Quinones and Trihydroxybenzenes AND Non-covalent interaction AND Non-covalent interaction >> DNA intercalation AND Non-covalent interaction >> DNA intercalation >> Quinones and Trihydroxybenzenes AND Radical AND Radical >> Radical mechanism via ROS formation (indirect) AND Radical >> Radical mechanism via ROS formation (indirect) >> Quinones and Trihydroxybenzenes by DNA binding by OASIS v.1.4

Domain logical expression index: "b"

Referential boundary: The target chemical should be classified as SN1 AND SN1 >> Nitrenium Ion formation AND SN1 >> Nitrenium Ion formation >> Secondary aromatic amine by DNA binding by OECD

Domain logical expression index: "c"

Referential boundary: The target chemical should be classified as AN2 AND AN2 >> Michael-type addition to quinoid structures  AND AN2 >> Michael-type addition to quinoid structures  >> N-Substituted Aromatic Amines by Protein binding by OASIS v1.4

Domain logical expression index: "d"

Referential boundary: The target chemical should be classified as Non binder, without OH or NH2 group by Estrogen Receptor Binding

Domain logical expression index: "e"

Referential boundary: The target chemical should be classified as Moderate binder, NH2 group OR Moderate binder, OH grooup OR Non binder, impaired OH or NH2 group OR Non binder, MW>500 OR Non binder, non cyclic structure OR Strong binder, NH2 group OR Strong binder, OH group OR Weak binder, NH2 group OR Weak binder, OH group by Estrogen Receptor Binding

Domain logical expression index: "f"

Referential boundary: The target chemical should be classified as AN2 AND AN2 >> Michael-type addition to quinoid structures  AND AN2 >> Michael-type addition to quinoid structures  >> N-Substituted Aromatic Amines by Protein binding by OASIS v1.4

Domain logical expression index: "g"

Referential boundary: The target chemical should be classified as Acylation OR Acylation >> Acylation involving an activated (glucuronidated) carboxamide group OR Acylation >> Acylation involving an activated (glucuronidated) carboxamide group >> Carboxylic Acid Amides OR Acylation >> Direct acylation involving a leaving group OR Acylation >> Direct acylation involving a leaving group >> Carbamates  OR Acylation >> Direct acylation involving a leaving group >> Carboxylic Acid Amides OR Acylation >> Ester aminolysis OR Acylation >> Ester aminolysis >> Amides OR Acylation >> Ester aminolysis or thiolysis OR Acylation >> Ester aminolysis or thiolysis >> Carbamates  OR AN2 >> Michael addition to activated double bonds OR AN2 >> Michael addition to activated double bonds >> alpha,beta-Unsaturated Carbonyls and Related Compounds OR AN2 >> Michael-type addition to quinoid structures  >> Carboxylic Acid Amides OR Michael addition OR Michael addition >> Michae addition on quinoide type compounds OR Michael addition >> Michae addition on quinoide type compounds >> Quinone methide(s)/imines; Quinoide oxime structure; Nitroquinones, Naphthoquinone(s)/imines  OR No alert found OR SN1 OR SN1 >> Carbenium ion formation (enzymatic) OR SN1 >> Carbenium ion formation (enzymatic) >> Carbenium ion OR SN2 OR SN2 >> Ring opening SN2 reaction OR SN2 >> Ring opening SN2 reaction >> Epoxides, Aziridines and Sulfuranes  OR SNVinyl OR SNVinyl >> SNVinyl at a vinylic (sp2) carbon atom OR SNVinyl >> SNVinyl at a vinylic (sp2) carbon atom >> Vinyl type compounds with electron withdrawing groups  by Protein binding by OASIS v1.4

Domain logical expression index: "h"

Referential boundary: The target chemical should be classified as No alert found by Protein binding by OECD

Domain logical expression index: "i"

Referential boundary: The target chemical should be classified as Acylation OR Acylation >> Direct Acylation Involving a Leaving group OR Acylation >> Direct Acylation Involving a Leaving group >> Acetates by Protein binding by OECD

Domain logical expression index: "j"

Referential boundary: The target chemical should be classified as Not bioavailable by Lipinski Rule Oasis ONLY

Domain logical expression index: "k"

Similarity boundary:Target: O=C1c2cccc(NC3CCCCC3)c2C(=O)c2cccc(NC3CCCCC3)c12
Threshold=40%,
Dice(Atom centered fragments)
Atom type; Count H attached; Hybridization

Domain logical expression index: "l"

Parametric boundary:The target chemical should have a value of log Kow which is >= 7.21

Domain logical expression index: "m"

Parametric boundary:The target chemical should have a value of log Kow which is <= 10.9

Interpretation of results:

other: not irritating

Conclusions:

 The substance 1,5-bis(cyclohexylamino)-9,10-anthraquinone (CAS No: 15958-68-6)was estimated to be not irritating to the skin of New Zealand White rabbits. Based on the estimated result 1,5-bis(cyclohexylamino)-9,10-anthraquinone (CAS No: 15958-68-6)can be considered as non irritant to skin.

Executive summary:

The dermal irritation potential of 1,5-bis(cyclohexylamino)-9,10-anthraquinone (CAS No: 15958-68-6)was estimated using OECD QSAR toolbox version 3.3 with logPow as the primary descriptor. The substance 1,5-bis(cyclohexylamino)-9,10-anthraquinone (CAS No: 15958-68-6)was estimated to be not irritating to the skin of New Zealand White rabbits. Based on the estimated result 1,5-bis(cyclohexylamino)-9,10-anthraquinone (CAS No: 15958-68-6)can be considered as non irritant to skin and can be classified under the category ˋ Not Classified’ as per CLP regulation.

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed (not irritating)

Eye irritation

Link to relevant study records

Reference

Endpoint:

eye irritation: in vivo

Type of information:

(Q)SAR

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

results derived from a valid (Q)SAR model and falling into its applicability domain, with limited documentation / justification

Justification for type of information:

Data is from OECD QSAR toolbox version 3.3 and QMRF report has been attached

Qualifier:

according to guideline

Guideline:

other: estimated data

Principles of method if other than guideline:

Prediction is done using QSAR Toolbox version 3.3

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material: 1,5-bis(cyclohexylamino)-9,10-dihydroanthracene-9,10-dione
- Molecular formula: C26H30N2O2
- Molecular weight: 402.535 g/mol
- Smiles notation: O=C1c2c(c(NC3CCCCC3)ccc2)C(=O)c2cccc(NC3CCCCC3)c12
- InChl: 1S/C26H30N2O2/c29-25-20-14-8-16-22(28-18-11-5-2-6-12-18)24(20)26(30)19-13-7-15-21(23(19)25)27-17-9-3-1-4-10-17/h7-8,13-18,27-28H,1-6,9-12H2
- Substance type: Organic
- Physical state: Solid

Species:

rabbit

Strain:

New Zealand White

Details on test animals or tissues and environmental conditions:

No data available

Vehicle:

not specified

Controls:

not specified

Amount / concentration applied:

No data available

Duration of treatment / exposure:

single exposure

Observation period (in vivo):

7 days

Number of animals or in vitro replicates:

3

Details on study design:

No data available

Irritation parameter:

overall irritation score

Basis:

mean

Time point:

7 d

Score:

0

Reversibility:

not specified

Remarks on result:

no indication of irritation

Irritant / corrosive response data:

no eye irritation was observed.

Estimation method: Takes mode value from the 7 nearest neighbours
Domain  logical expression:Result: In Domain

((((((("a" or "b" or "c" )  and ("d" and ( not "e") )  )  and ("f" and ( not "g") )  )  and ("h" and ( not "i") )  )  and ("j" and ( not "k") )  )  and "l" )  and ("m" and "n" )  )

Domain logical expression index: "a"

Referential boundary: The target chemical should be classified as AN2 AND AN2 >>  Michael-type addition, quinoid structures AND AN2 >>  Michael-type addition, quinoid structures >> Quinones and Trihydroxybenzenes AND Non-covalent interaction AND Non-covalent interaction >> DNA intercalation AND Non-covalent interaction >> DNA intercalation >> Quinones and Trihydroxybenzenes AND Radical AND Radical >> Radical mechanism via ROS formation (indirect) AND Radical >> Radical mechanism via ROS formation (indirect) >> Quinones and Trihydroxybenzenes by DNA binding by OASIS v.1.4

Domain logical expression index: "b"

Referential boundary: The target chemical should be classified as SN1 AND SN1 >> Nitrenium Ion formation AND SN1 >> Nitrenium Ion formation >> Secondary aromatic amine by DNA binding by OECD

Domain logical expression index: "c"

Referential boundary: The target chemical should be classified as AN2 AND AN2 >> Michael-type addition to quinoid structures  AND AN2 >> Michael-type addition to quinoid structures  >> N-Substituted Aromatic Amines by Protein binding by OASIS v1.4

Domain logical expression index: "d"

Referential boundary: The target chemical should be classified as Non binder, without OH or NH2 group by Estrogen Receptor Binding

Domain logical expression index: "e"

Referential boundary: The target chemical should be classified as Moderate binder, NH2 group OR Non binder, impaired OH or NH2 group OR Non binder, MW>500 OR Non binder, non cyclic structure OR Strong binder, NH2 group OR Strong binder, OH group OR Weak binder, OH group by Estrogen Receptor Binding

Domain logical expression index: "f"

Referential boundary: The target chemical should be classified as AN2 AND AN2 >> Michael-type addition to quinoid structures  AND AN2 >> Michael-type addition to quinoid structures  >> N-Substituted Aromatic Amines by Protein binding by OASIS v1.4

Domain logical expression index: "g"

Referential boundary: The target chemical should be classified as Acylation OR Acylation >> Acylation involving an activated (glucuronidated) carboxamide group OR Acylation >> Acylation involving an activated (glucuronidated) carboxamide group >> Carboxylic Acid Amides OR Acylation >> Direct acylation involving a leaving group OR Acylation >> Direct acylation involving a leaving group >> Carbamates  OR Acylation >> Direct acylation involving a leaving group >> Carboxylic Acid Amides OR Acylation >> Ester aminolysis OR Acylation >> Ester aminolysis >> Amides OR Acylation >> Ester aminolysis or thiolysis OR Acylation >> Ester aminolysis or thiolysis >> Carbamates  OR AN2 >> Michael addition to activated double bonds OR AN2 >> Michael addition to activated double bonds >> alpha,beta-Unsaturated Carbonyls and Related Compounds OR AN2 >> Michael-type addition to quinoid structures  >> Carboxylic Acid Amides OR Michael addition OR Michael addition >> Michae addition on quinoide type compounds OR Michael addition >> Michae addition on quinoide type compounds >> Quinone methide(s)/imines; Quinoide oxime structure; Nitroquinones, Naphthoquinone(s)/imines  OR Michael addition >> Michael addition on conjugated systems with electron withdrawing group OR Michael addition >> Michael addition on conjugated systems with electron withdrawing group >> alpha,beta-Carbonyl compounds with polarized double bonds  OR No alert found OR SN1 OR SN1 >> Carbenium ion formation (enzymatic) OR SN1 >> Carbenium ion formation (enzymatic) >> Carbenium ion OR SNVinyl OR SNVinyl >> SNVinyl at a vinylic (sp2) carbon atom OR SNVinyl >> SNVinyl at a vinylic (sp2) carbon atom >> Vinyl type compounds with electron withdrawing groups  by Protein binding by OASIS v1.4

Domain logical expression index: "h"

Referential boundary: The target chemical should be classified as No alert found by Protein binding by OECD

Domain logical expression index: "i"

Referential boundary: The target chemical should be classified as Acylation OR Acylation >> Direct Acylation Involving a Leaving group OR Acylation >> Direct Acylation Involving a Leaving group >> Acetates by Protein binding by OECD

Domain logical expression index: "j"

Referential boundary: The target chemical should be classified as No alert found by Protein binding alerts for Chromosomal aberration by OASIS v.1.2

Domain logical expression index: "k"

Referential boundary: The target chemical should be classified as AN2 OR AN2 >> Michael addition to the quinoid type structures OR AN2 >> Michael addition to the quinoid type structures >> N-Subsituted Aromatic Amines by Protein binding alerts for Chromosomal aberration by OASIS v.1.2

Domain logical expression index: "l"

Referential boundary: The target chemical should be classified as Not bioavailable by Lipinski Rule Oasis ONLY

Domain logical expression index: "m"

Parametric boundary:The target chemical should have a value of log Kow which is >= 5.56

Domain logical expression index: "n"

Parametric boundary:The target chemical should have a value of log Kow which is <= 11.9

Interpretation of results:

other: not irritating

Conclusions:

The substance 1,5-bis(cyclohexylamino)-9,10-anthraquinone (CAS No: 15958-68-6)was estimated to be not irritating into the eyes of New Zealand White rabbits. Based on the estimated result 1,5-bis(cyclohexylamino)-9,10-anthraquinone (CAS No: 15958-68-6)can be considered to be not irritating to eye.

Executive summary:

The ocular irritation potential of 1,5-bis(cyclohexylamino)-9,10-anthraquinone (CAS No: 15958-68-6)was estimated using OECD QSAR toolbox version 3.3 with logPow as the primary descriptor. The substance 1,5-bis(cyclohexylamino)-9,10-anthraquinone (CAS No: 15958-68-6)was estimated to be not irritating into the eyes of New Zealand White rabbits. Based on the estimated result 1,5-bis(cyclohexylamino)-9,10-anthraquinone (CAS No: 15958-68-6)can be considered to be not irritating to eye and can be classified under the category ˋ Not Classified’ as per CLP regulation.

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed (not irritating)

Respiratory irritation

Endpoint conclusion

Endpoint conclusion:

no study available

Additional information

Skin irritation:

Various studieshas been investigated for the test chemical1,5-bis(cyclohexylamino)-9,10-anthraquinone (CAS No: 15958-68-6)to observe the potential for dermal irritation to a greater or lesser extent. The studies are based on in vivo experiments in rabbits for target chemical1,5-bis(cyclohexylamino)-9,10-anthraquinone (CAS No: 15958-68-6)and its structurally similar read across substances2-Ethylanthraquinone (CAS No: 84-51-5)andC.I. Acid Blue 62 (CAS No: 4368-56-3).The predicted data using the OECD QSAR toolbox has also been compared with the experimental data and summarized as below;

 

In a prediction done by SSS (2017) using the OECD QSAR toolbox with log kow as the primary descriptor, the skin irritation potential was estimated for test chemical1,5-bis(cyclohexylamino)-9,10-anthraquinone (CAS No: 15958-68-6) .The chemical 1,5-bis(cyclohexylamino)-9,10-anthraquinone (CAS No: 15958-68-6)is estimated to be not irritating to skin of New Zealand White rabbits.

 

The NTRL Reported (1991) the skin irritation potential ofstructurally similar read across substance2-Ethylanthraquinone (CAS No: 84-51-5) in albino rabbits according to the Draize method which supports the above result. In this study, 6 albino New Zealand rabbits (3/sex) were used for the study. 0.5 ml (0.5 g) of the test material was applied to clipped areas of intact and abraded skin. The abrasions were longitudinal epidermal incisions sufficiently deep to penetrate the stratum corneum, but not so deep as to destroy the integrity of the derma. Applications were made under occlusive patches (1" x 1" gauze, covered by adhesive tape). Following application of 2-ethylanthraquinone the entire trunk of each animal was covered with an impermeable occlusive wrapping. The animals were then immobilized. The wrapping and test material were removed 24 hours following application. The sites were individually examined and scored separately for erythema and edema at 24 and 72 hours. The mean scores for 24 and 72 hours grading were averaged to determine final irritation indices. The Primary Irritation Index after 24, 72 hours for 2-ethylanthraquinone was 0.15. Based on the score and scale of interpretation of PII scores, 2-Ethylanthraquinone (CAS No: 84-51-5) can be considered not irritating to skin.

 

The above results were further supported by the experimental study conducted by EUROPEAN COMMISSION HEALTH & CONSUMER PROTECTION DIRECTORATE-GENERAL (2005) forstructurally similar read across substance C.I. Acid Blue 62 (CAS No: 4368-56-3) on intact and scarified skin of six male New Zealand White rabbits to examine its eye irritancy potential.During the study, Acid Blue 62 (purity unknown) was applied (0.5 ml) at a concentration of 1.5% in water on two gauze pads, 2 cm² in area, with one pad placed on the left flank and the other on the scarified area of the right flank under occlusive condition by using an adhesive bandage.Twenty-three (23) hours later, the patches were removed, and 1 hour after this, the skin was evaluated for possible lesions. Skin biopsies were taken from the right flank of all animals at this time. Application sites were evaluated again 48 hours later (72 hours after application of the test substance), and skin biopsies from the left flank were taken at this time.There was no evidence of oedema at any of the patch test sites at the 24- and 72-hour observation periods. The compound discoloured the application sites such that erythema could not be evaluated; thus the skin biopsies were examined. There were no histopathological abnormalities noted in the skin biopsies.Hence the chemicalC.I. Acid Blue 62 (CAS No: 4368-56-3)was considered to be non irritant to intact and scarified rabbit skin ofNew Zealand Whiterabbits.

 

Thus on the basis of available data for thetarget chemical1,5-bis(cyclohexylamino)-9,10-anthraquinone (CAS No: 15958-68-6)and its structurally similar read across substances2-Ethylanthraquinone (CAS No: 84-51-5)andC.I. Acid Blue 62 (CAS No: 4368-56-3),it can be concluded thatchemical 1,5-bis(cyclohexylamino)-9,10-anthraquinone (CAS No: 15958-68-6)is unable to cause skin irritation and considered as not irritating.Comparing the above annotations with the criteria of CLP regulation, it can be classified under the category “Not Classified”.

 

 

Eye irritation:

In different studies,the test chemical1,5-bis(cyclohexylamino)-9,10-anthraquinone (CAS No: 15958-68-6)has been investigated for potential for ocular irritationto a greater or lesser extent. The studies are based on in vivo experiments in rabbits for target chemical1,5-bis(cyclohexylamino)-9,10-anthraquinone (CAS No: 15958-68-6) andits structurally similar read across substances 2-Ethylanthraquinone (CAS No: 84-51-5)and9,10–anthraquinone (CAS No: 84-65-1).The predicted data using the OECD QSAR toolbox has also been compared with the experimental data and summarized as below;

 

 

In a prediction done by SSS (2017) using the OECD QSAR toolbox with log kow as the primary descriptor, the eye irritation potential was estimated for test chemical1,5-bis(cyclohexylamino)-9,10-anthraquinone (CAS No: 15958-68-6).The chemical 1,5-bis(cyclohexylamino)-9,10-anthraquinone (CAS No: 15958-68-6)is estimated to be not irritating to eye of New Zealand White rabbits.

 

The JOSE J. ESTABLE (1948) conducted an eye irritation study to compare the immediate and delayed effects ofstructurally similar read across substance9,10–anthraquinone (CAS No: 84-65-1) on the cornea—a tissue normally devoid of blood vessels with their effects on the vascular tissues of the sclera and eyelids. White rabbits were used for the study. Anthraquinone(dry powder)was placed in the lower conjunctival sac of one eye and held there usually for one-half minute. This was repeated at intervals of one or several days.The other eye was treated with 0.7% solution of Sodium chloride and used as control.The eyes were examined for evidence of inflammation, and changes in the corneal structures were observed with the bio-microscope. Anthraquinone produced only immediate sensory and inflammatory reaction (discomfort, blepharospasm, and conjunctival congestion) that disappeared very soon. The eyes appeared normal in a few hours. Hence, it was concluded the irritation effects were due to mechanical action of the powder rather than toxic effects of anthraquinone. Therefore, Anthraquinone (CAS No: 84-65-1) can be considered not irritating to eyes.

 

The above results were further supported by an ocular irritation study reported by NTRL (1991) onstructurally similar read across substance2-ethylanthraquionone (CAS No: 84-51-5) in albino rabbits according to the Draize method.9 albino New Zealand rabbits (mixed sex), weighing approximately 1.8- 2.4 kg were used for the study. One tenth (0.1) of a millilitre of the test substance was instilled in the right eye; the left eye, remaining untreated served as a control. The treated eyes of six rabbits remained unwashed for 24 hours. The eyes of the remaining three (3) rabbits were irrigated 4 seconds following instillation of the test material (30 ml tap water at 37 degrees C). Readings facilitated by hand-held lenses were made 1, 2, 3, and up to 7 days after treatment. The scores of washed and unwashed eyes of rabbits after 3 days of observation were 0, 0 respectively. Based on the scores, 2-ethylanthraquinone (CAS No: 84-51-5) can be considered not irritating to eyes

 

Thus on the basis of available data for thetarget chemical1,5-bis(cyclohexylamino)-9,10-anthraquinone (CAS No: 15958-68-6)and its structurally similar read across substances2-Ethylanthraquinone (CAS No: 84-51-5)and9,10–anthraquinone (CAS No: 84-65-1),it can be concluded thatchemical 1,5-bis(cyclohexylamino)-9,10-anthraquinone (CAS No: 15958-68-6)is unable to cause eye irritation and considered as not irritating.Comparing the above annotations with the criteria of CLP regulation, it can be classified under the category “Not Classified”.

 

Justification for classification or non-classification

The skin and eye irritation potential of test chemical1,5-bis(cyclohexylamino)-9,10-anthraquinone (CAS No: 15958-68-6)and its structurally similar read across substanceswere observed in various studies. The results obtained from these studies indicate that the chemical1,5-bis(cyclohexylamino)-9,10-anthraquinoneis unlikely to cause skin and eye irritation. Hence1,5-bis(cyclohexylamino)-9,10-anthraquinone (CAS No: 15958-68-6)can be classified under the category “Not Classified” for skin and eye as per CLP.