2-(3-methoxypropyl)-9(or 10)-methylbenzimidazo[2,1-b]benzo[lmn][3,8]phenanthroline-1,3,6(2H)-trione

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

26 - 30 Apr 1982

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Remarks:

Use of dispersant caused higher exposure than environmentally relevant

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1982

Report date:

1982

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2-(3-methoxypropyl)-9(or 10)-methylbenzimidazo[2,1-b]benzo[lmn][3,8]phenanthroline-1,3,6(2H)-trione

EC Number:

258-094-6

EC Name:

2-(3-methoxypropyl)-9(or 10)-methylbenzimidazo[2,1-b]benzo[lmn][3,8]phenanthroline-1,3,6(2H)-trione

Cas Number:

52673-15-1

Molecular formula:

C25H19N3O4

IUPAC Name:

17-(3-methoxypropyl)-6(or7)-methyl-3,10,17-triazahexacyclo[13.6.2.0²,¹.0,.0¹²,²².0¹,²³]tricosa-1(21),2,4(9),5,7,12(22),13,15(23),19-nonaene-11,16,18-trione

Test material form:

solid: particulate/powder

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

Stock solution (500 mg/L) prepared with dilution water and respective amount added to test vessels

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: zebra fish
- Source: Hoechst AG
- Age at study initiation (mean and range, SD): ca. 6 months
- Length at study initiation (length definition, mean, range and SD): 2.9 - 3.5 cm, mean 3.3 cm, SD = 0.21 cm

ACCLIMATION
- Acclimation period: 7 d
- Acclimation conditions (same as test or not): same
- Type and amount of food during acclimation: ad lib. TetraMin
- Feeding frequency during acclimation: twice per day

FEEDING DURING TEST
No feeding during test

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

2.5 mmol/L (Ca/Mg)

Test temperature:

22.2 - 23.9°C

pH:

7.6 - 8.3

Dissolved oxygen:

6.6 - 8.8 mg/L

Nominal and measured concentrations:

Nominal: 0 (control), 1, 10, 100, 500 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: aquaria
- Material, size, headspace, fill volume: glass, 10 L fill volume
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 12/12
- Light intensity: ca. 700 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality, visual observations of behaviour and appearance at 2, 4, 24, 48, 72 and 96 h

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

No mortality occured in negative control and test concentrations.
No behavioural abnormalities in 1 and 10 mg/L; no realiable behaviour observation in higher test concentrations due to strong orange colouration of test solutions.

Results with reference substance (positive control):

NA

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The test item is not acutely toxic to fish. No mortality occured in an acute toxicity test with D. rerio up to 500 mg/L. The test item was dispersed in the test solutions. Under environmentally relevant conditions, the water solubility limit is far below the LC0 (500 mg/L, nominal).