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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
06/06/2000 - 08/06/2000
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study according to international guideline (EU Method C2) under GLP. Reliability 2 is assigned as test item concentrations were not verified.
Reason / purpose for cross-reference:
reference to same study
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
no
Principles of method if other than guideline:
Not relevant
GLP compliance:
yes (incl. QA statement)
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
Not relevant
Analytical monitoring:
no
Details on sampling:
Not relevant
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: To produce the test concentrations, the test substance was weighed into water, treated for 60 seconds at 8000 rpm with an ultra turrax and afterwards stirred for 24 hours with a magnetic stirrer. To remove undissolved particles, the resulting emulsions were filtered using folded filters of pore size 7-12 um. Considering the heterogeneous composition of the test substance, Water Accommodated Fractions (=WAF) of the test substance were tested.
- Controls: yes, medium blanks
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna
- Strain: Straus
- Source: cloned at the Federal Health Office (BGA) in Berlin, test individuals were laboratory bred at Bayer AG Leverkusen
- Age at study initiation (mean and range, SD): 0-24 hours
- Method of breeding: internal standard operating procedure (SOP 2030-7700204-98 D)
- Feeding during test: No
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
Not relevant
Hardness:
13.5 ºdH
Test temperature:
20.1 - 21.0ºC
pH:
7.8 - 7.9
Dissolved oxygen:
8.8 - 9.0 mg/L
Salinity:
Not relevant
Nominal and measured concentrations:
Nominal WAFs of 2.5, 5, 10, 20 and 40 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: cylindrical test vessels
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: 80 mL volume, filled with 20 mL test medium
- Aeration: No
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates):2

TEST MEDIUM / WATER PARAMETERS
- Medium: Elendt M4 medium
- Culture medium different from test medium: No
- Intervals of water quality measurement: only reported after 48 h

OTHER TEST CONDITIONS
- Photoperiod: 16 h light / 8 h dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): immobility (24 h)

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
Reference substance (positive control):
no
Duration:
24 h
Dose descriptor:
EL50
Effect conc.:
20 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: Geometric mean of EL0 and El100
Duration:
48 h
Dose descriptor:
EL50
Effect conc.:
20 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: Geometric mean of EL0 and El100
Duration:
24 h
Dose descriptor:
EL0
Effect conc.:
10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EL0
Effect conc.:
10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EL100
Effect conc.:
40 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EL100
Effect conc.:
40 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
- Mortality of control: 0
Results with reference substance (positive control):
Not relevant
Reported statistics and error estimates:
EL50s were calculated as the geometric mean of the EL0 and EL100.

Nominal WAF concentration (mg/L)

Number of immobilized Daphnids

Percentage of immobilized Daphnids

Oxygen (mg/L) after 48 h

pH after 48 h

Temperature (ºC) after 48 h

24 h

48 h

24 h

48 h

Control

0

0

0

0

8.9

7.9

20.3

2.5

0

0

0

0

9.0

7.9

20.1

5

0

0

0

0

8.9

7.9

20.1

10

0

0

0

0

9.0

7.9

20.2

20

14

15

70

75

8.0

7.9

20.2

40

20

 

100

 

8.8

7.8

21.0

Validity criteria fulfilled:
yes
Remarks:
0% mortality in controls, physical-chemical parameters within acceptable range.
Conclusions:
The acute toxicity (48h-EL50) of Eucalyptus maculata citriodora, ext. towards Daphnia magna is 20 mg/L.
Executive summary:

The acute toxicity of Eucalyptus maculata citriodora, ext. towards Daphnia magna was investigated according to EU Method C2 under GLP. Daphnids were exposed to nominal WAFs of 2.5, 5, 10, 20 and 40 mg/L and were observed for 48 hours. Based on the geometric mean of the 48h-EL0 and 48h-EL100 the 48h-EL50 was found to be 20 mg/L.

Description of key information

48h-EL50: 20 mg/L

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
20 mg/L

Additional information

The acute toxicity of Eucalyptus maculata citriodora, ext. towards Daphnia magna was investigated according to EU Method C2 under GLP. Daphnids were exposed to nominal WAFs of 2.5, 5, 10, 20 and 40 mg/L and were observed for 48 hours. Based on the geometric mean of the 48h-EL0 and 48h-EL100 the 48h-EL50 was found to be 20 mg/L.

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