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EC number: 305-053-6 | CAS number: 94333-72-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 016
- Report date:
- 2016
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Citronellol
- EC Number:
- 203-375-0
- EC Name:
- Citronellol
- Cas Number:
- 106-22-9
- Molecular formula:
- C10H20O
- IUPAC Name:
- 3,7-dimethyloct-6-en-1-ol
- Reference substance name:
- Geraniol
- EC Number:
- 203-377-1
- EC Name:
- Geraniol
- Cas Number:
- 106-24-1
- Molecular formula:
- C10H18O
- IUPAC Name:
- (E)-3,7-dimethylocta-2,6-dien-1-ol
- Reference substance name:
- (E)-6,10-dimethylundeca-3,9-dien-2-one
- Cas Number:
- 19870-49-6
- Molecular formula:
- C12H20O
- IUPAC Name:
- (E)-6,10-dimethylundeca-3,9-dien-2-one
- Reference substance name:
- Dipentene
- EC Number:
- 205-341-0
- EC Name:
- Dipentene
- Cas Number:
- 138-86-3
- Molecular formula:
- C10H16
- IUPAC Name:
- 1-methyl-4-(prop-1-en-2-yl)cyclohex-1-ene
- Reference substance name:
- Linalool
- EC Number:
- 201-134-4
- EC Name:
- Linalool
- Cas Number:
- 78-70-6
- Molecular formula:
- C10H18O
- IUPAC Name:
- 3,7-dimethylocta-1,6-dien-3-ol
- Reference substance name:
- (±)-(1α,2β,5α)-5-methyl-2-(1-methylvinyl)cyclohexan-1-ol
- EC Number:
- 256-557-7
- EC Name:
- (±)-(1α,2β,5α)-5-methyl-2-(1-methylvinyl)cyclohexan-1-ol
- Cas Number:
- 50373-36-9
- Molecular formula:
- C10H18O
- IUPAC Name:
- rel-(1R,2S,5R)-5-methyl-2-(prop-1-en-2-yl)cyclohexanol
- Reference substance name:
- (1α,2β,4β)-1-methyl-2,4-bis(methylvinyl)-1-vinylcyclohexane
- EC Number:
- 251-713-0
- EC Name:
- (1α,2β,4β)-1-methyl-2,4-bis(methylvinyl)-1-vinylcyclohexane
- Cas Number:
- 33880-83-0
- Molecular formula:
- C15H24
- IUPAC Name:
- rel-(1R,2R,4S)-1-methyl-2,4-di(prop-1-en-2-yl)-1-vinylcyclohexane
- Reference substance name:
- (1E,6E)-8-isopropyl-1-methyl-5-methylenecyclodeca-1,6-diene
- Cas Number:
- 105453-16-5
- Molecular formula:
- C15H24
- IUPAC Name:
- (1E,6E)-8-isopropyl-1-methyl-5-methylenecyclodeca-1,6-diene
- Reference substance name:
- rel-(1R,4aR,8aS)-1-isopropyl-4,7-dimethyl-1,2,4a,5,6,8a-hexahydronaphthalene
- Cas Number:
- 31983-22-9
- Molecular formula:
- C15H24
- IUPAC Name:
- rel-(1R,4aR,8aS)-1-isopropyl-4,7-dimethyl-1,2,4a,5,6,8a-hexahydronaphthalene
- Reference substance name:
- rel-(1R,4aS,8aS)-1-isopropyl-7-methyl-4-methylene-1,2,3,4,4a,5,6,8aoctahydronaphthalene
- Cas Number:
- 39029-41-9
- Molecular formula:
- C15H24
- IUPAC Name:
- rel-(1R,4aS,8aS)-1-isopropyl-7-methyl-4-methylene-1,2,3,4,4a,5,6,8aoctahydronaphthalene
- Reference substance name:
- rel-(1R,8aS)-1-isopropyl-4,7-dimethyl-1,2,3,5,6,8ahexahydronaphthalene
- Cas Number:
- 60305-17-1
- Molecular formula:
- C15H24
- IUPAC Name:
- rel-(1R,8aS)-1-isopropyl-4,7-dimethyl-1,2,3,5,6,8ahexahydronaphthalene
- Reference substance name:
- rel-2-((1R,3S,4S)-4-methyl-3-(prop-1-en-2-yl)-4-vinylcyclohexyl)propan-2-ol
- Cas Number:
- 23811-48-5
- Molecular formula:
- C15H26O
- IUPAC Name:
- rel-2-((1R,3S,4S)-4-methyl-3-(prop-1-en-2-yl)-4-vinylcyclohexyl)propan-2-ol
- Reference substance name:
- 5,9-dimethyl-2-(6-methylhept-5-en-2-yl)deca-2,8-dienal
- Cas Number:
- 82451-80-7
- Molecular formula:
- C20H34O
- IUPAC Name:
- 5,9-dimethyl-2-(6-methylhept-5-en-2-yl)deca-2,8-dienal
- Reference substance name:
- Likely oxygenated constituent
- IUPAC Name:
- Likely oxygenated constituent
- Reference substance name:
- Unknown Impurities
- IUPAC Name:
- Unknown Impurities
- Test material form:
- liquid
Constituent 1
Constituent 2
Constituent 3
Constituent 4
Constituent 5
Constituent 6
Constituent 7
Constituent 8
Constituent 9
Constituent 10
Constituent 11
Constituent 12
Constituent 13
Constituent 14
Constituent 15
- Specific details on test material used for the study:
- Identity: BACCARTOL CRUDE
Batch no.: VE00364716
Expiry date: 02 April 2017
Storage conditions: Room temperature, protected from light
RTC number: 14844
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2
- Metabolic activation:
- with and without
- Test concentrations with justification for top dose:
- Toxicity Test
The test item BACCARTOL CRUDE was assayed in the toxicity test at a maximum concentration of 5000 µg/plate and at four lower concentrations spaced at approximately half-log intervals: 1580, 500, 158 and 50.0 µg/plate. No precipitation of the test item was observed at the end of the incubation period at any concentration.
Main Assays
Three Main Assays were performed. The maximum concentration of the test item to be used in the main experiments was selected as the concentration which elicits a moderate toxicity. The number of lower dose levels included in each treatment series was selected in order to have a sufficient number of analysable concentrations (at least three concentrations without toxicity).
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- 2-nitrofluorene
- sodium azide
- methylmethanesulfonate
- other: 2-Aminoanthracene
- Details on test system and experimental conditions:
- One batch of S9 tissue fraction, provided by Trinova Biochem GmbH, was used in this study and had the following characteristics:
Species: Rat
Strain: Sprague Dawley
Tissue: Liver
Inducing Agents: Phenobarbital – 5,6-Benzoflavone
Producer: MOLTOX,Molecular Toxicology, Inc.
Batch Number: 3488 - Evaluation criteria:
- Acceptance criteria
The assay was considered valid if the following criteria were met:
1. Mean plate counts for untreated and positive control plates should fall within 2 standard deviations of the current historical mean values.
2. The estimated numbers of viable bacteria/plate should fall in the range of 100 – 500 millions for each strain.
3. No more than 5% of the plates should be lost through contamination or other unforeseen event.
Criteria for outcome of the assays
For the test item to be considered mutagenic, two-fold (or more) increases in mean revertant numbers must be observed at two consecutive dose levels or at the highest practicable dose level only. In addition, there must be evidence of a dose-response relationship showing increasing numbers of mutant colonies with increasing dose levels.
Evaluation
Results show that mean plate counts for untreated and positive control plates fell within the normal range based on historical control data. The estimated numbers of viable bacteria/plate (titre) fell in the range of 100 - 500 million for each strain. No plates were lost through contamination or cracking. The study was accepted as valid.
The test item did not induce two-fold increases in the number of revertant colonies, at any dose level, in any tester strain, in the absence or presence of S9 metabolism.
Results and discussion
Test results
- Species / strain:
- other: TA1535, TA1537, TA98, TA100 and a strain of Escherichia coli (WP2 uvrA)
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- Toxicity test
No precipitation of the test item was observed at the end of the incubation period at any concentration. Toxicity, as indicated by reduction in revertant colonies, thinning of the background lawn and/or microcolony formation was noted at higher concentrations with all tester strains, in the absence and presence of S9 metabolism. A more pronounced toxic effect was noted with TA100 tester strain.
Main Assays
In Main Assay I, using the plate incorporation method, the test item was assayed at the following dose levels:
Tester strain S9 Dose level (µg/plate)
TA1535, TA1537 ± 2500, 1250, 625, 313 and 156
TA98 ± 5000, 2500, 1250, 625, 313 and 156
WP2 uvrA − 5000, 2500, 1250, 625, 313 and 156
WP2 uvrA + 5000, 2500, 1250, 625 and 313
TA100 − 2500, 1250, 625, 313, 156, 78.1 and 39.1
TA100 + 2500, 1250, 625, 313, 156 and 78.1
Toxicity, as indicated by thinning of the background lawn and/or reduction in revertant number, was observed at dose levels of 2500 and 1250 µg/plate with TA1535, TA 1537 and TA100 tester strains, both in the absence and presence of S9 metabolic activation. A more pronounced toxic effect was observed with TA100 tester strain in the absence of S9 metabolism, where slight thinning of the background lawn was noted also at 625 µg/plate. No toxicity was observed with WP2 uvrA, while slight toxicity was noted with TA98 tester strain at 5000 µg/plate, both in the absence and presence of S9 metabolism. As no relevant increase in revertant numbers was observed at any concentration tested, a pre-incubation step was included for all treatments of Main Assay II. The dose-range was slightly modified to take into account the toxicity results of Main Assay I.
Main Assay II:
Tester strain S9 Dose level (µg/plate)
TA1535, TA1537 ± 2000, 1000, 500, 250, 125 and 62.5
TA100 ± 2000, 1000, 500, 250, 125, 62.5 and 31.3
WP2 uvrA ± 5000, 2500, 1250, 625 and 313
TA98 ± 5000, 2500, 1250, 625, 313 and 156
Toxicity, as indicated by thinning of the background lawn, microcolony formation and/or reduction in revertant number, was observed at all or almost all concentrations tested, in the absence and presence of S9 metabolism, with all tester strains with the exception of TA100, where a sufficient number of analysable concentrations was obtained. In order to analyse the test item at not cytotoxic concentration, a Main Assay III was performed with TA1535, TA1537, TA98 and WP2 uvrA tester strains, in the absence and presence of S9 metabolism, using the pre-incubation method.
Main Assay III:
Tester strain S9 Dose level (µg/plate)
TA1535, TA1537 − 250, 125, 62.5, 31.3 and 15.6
TA1535, TA1537 + 62.5, 31.3, 15.6, 7.81, 3.91 and 1.95
WP2 uvrA, TA98 + 250, 125, 62.5, 31.3, 15.6 and 7.81
WP2 uvrA − 500, 250, 125, 62.5, 31.3 and 15.6
TA98 − 500, 250, 125, 62.5 and 31.3
Toxicity, as indicated by thinning of the background lawn, was observed at the highest or two highest dose levels with all tester strains, in the absence and presence of S9 metabolism. No precipitation of the test item was observed at the end of the incubation period at any
concentration, in any experiment. No relevant increase in the number of revertant colonies was observed in any experiment, using the plate incorporation or pre-incubation method, at any dose level, with any tester strain, in the absence or presence of S9 metabolism.
The sterility of the S9 mix and of the test item solutions was confirmed by the absence of colonies on additional agar plates spread separately with these solutions. Marked increases in revertant numbers were obtained in these tests following treatment with the positive control items, indicating that the assay system was functioning correctly. - Remarks on result:
- other: results provided in 'Additional information on results'
Applicant's summary and conclusion
- Conclusions:
- It is concluded that the test item BACCARTOL CRUDE does not induce reverse mutation in Salmonella typhimurium or Escherichia coli in the absence or presence of S9 metabolism, under the reported experimental conditions.
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