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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)
Analytical monitoring:
yes
Vehicle:
yes
Remarks:
water
Details on test solutions:
The stock solution of the test substance was prepared in the dilution water. 100 mg of the test substance was weighed into 1000 mL of the dilution water for the preliminary test. The stock solution was ultrasonicated for 10 minutes. The concentrations of solutions used in the preliminary test were obtained by dilution of the stock solution with dilution water.
Test organisms (species):
Daphnia magna
Details on test organisms:
The organism used in this test system is the species Daphnia magna. Daphnia magna Straus were obtained by aperiodic parthenogenesis from own laboratory breeding. Daphnia were fed by a mixture of algae. The sensitivity of the test species is periodically monitored by testing with the reference substance, potassium dichromate.
The grow-up gravid females are separated into dilution water. Young daphnia born in 24 hours are segregated to the handling container from which they entered the experiment. At handling a care should be taken of not to penetrate the air under animal’s shell.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
2.47 mmol/l Ca+Mg
Test temperature:
18-22°C
pH:
100 mg/l 48 h: 7.9
100 mg/l 0 h: 8.3
Dissolved oxygen:
The medium was oxygen saturated at the beginning of the test.
Nominal and measured concentrations:
1, 5, 10, 50 and 100 mg/l
Details on test conditions:
Temperature: 18-22 °C, with a variation of 1°C at each individual test
Exposure time: 48 hours
Lighting: 16-hours light and 8-hours dark
Number of test animals: 20 for each concentration and control
Volume of test substance solution: 50 ml (least 2 ml per individual)
Concentration of dissolved oxygen: higher than 3 mg·l -1
Reference substance (positive control):
yes
Remarks:
Potassium dichromate
Duration:
24 h
Dose descriptor:
EC0
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC100
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Validity criteria fulfilled:
yes
Conclusions:
EC50 48 h > 100 mg/l
Executive summary:

No toxic effects on Daphnia was observed under the test concentrations.

Description of key information

EC50 = 100 mg/l

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
100 mg/L

Additional information

One test on Daphnia Magna have been performed, showing no mortality at the tested concentrations.

The key study has been performed for different concentration from 0 to 100 mg/l based on the active ingredient.
The value of EC50 > 100 mg/l has been established.