2-benzylideneheptanal

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

No data

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Justification for type of information:

Peer reviewed journal research article

Data source

Reference

Reference Type:

publication

Title:

Study of artificial flavouring substances for mutagenicity in the Salmonella/Microsome Basc and Micronucleus tests

Author:

D. Wild, M. T. King, E. Gocke and K. Eckhardt

Year:

1983

Bibliographic source:

Food and chemical toxicology, Vol. 21, 6, 707-719, 1983

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Refer below principle

Deviations:

not specified

Principles of method if other than guideline:

The mutagenicity potential of α-Amylcinnamaldehyde by conducting experiment in Salmonella typhimurium TA1535, TA100, TA1537, TA1538 and TA 98.

GLP compliance:

not specified

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

2-benzylideneheptanal

EC Number:

204-541-5

EC Name:

2-benzylideneheptanal

Cas Number:

122-40-7

Molecular formula:

C14H18O

IUPAC Name:

2-benzylideneheptanal

Test material form:

liquid

Details on test material:

Name of test material : (2E)-2-(phenylmethylidene)heptanal
Common name : 2-benzylideneheptanal
Molecular formula : C14H18O
Molecular weight : 202.2952 g/mol
Physical appearence: Liquid
Smiles notation : c1(\C=C(\CCCCC)C=O)ccccc1
InChl : 1S/C14H18O/c1-2-3-5-10-14(12-15)11-13-8-6-4-7-9-13/h4,6-9,11-12H,2-3,5,10H2,1H3/b14-11-
Substance type : Organic

Specific details on test material used for the study:

- Name of test material (as cited in study report): α-Amylcinnamaldehyde
- Molecular formula (if other than submission substance): C14H18O
- Molecular weight (if other than submission substance): 202.2952 g/mol
- Substance type: Organic
- Physical state: No data
- Purity: No data
- Impurities (identity and concentrations): No data

Method

Target gene:

Histidine

Species / strain

Species / strain / cell type:

other: Species: Salmonella typhimurium Strain: TA1535, TA100, TA1537, TA1538, TA98

Details on mammalian cell type (if applicable):

Not applicable.

Additional strain / cell type characteristics:

not specified

Cytokinesis block (if used):

not specified

Metabolic activation:

with and without

Metabolic activation system:

S9 mixture from liver fraction of rat pretreated with Aroclor 1254

Test concentrations with justification for top dose:

Up to 3.6 mg/plate

Vehicle / solvent:

- Vehicle(s)/solvent(s) used: DMSO
- Justification for choice of solvent/vehicle: Solubility of test substance in DMSO solvent.

Controls

Untreated negative controls:

no

Negative solvent / vehicle controls:

yes

True negative controls:

not specified

Positive controls:

yes

Positive control substance:

sodium azide

benzo(a)pyrene

Details on test system and experimental conditions:

METHOD OF APPLICATION: Plate incorporation

DURATION
- Preincubation period: No data
- Exposure duration: 48 hrs
- Expression time (cells in growth medium): No data
- Selection time (if incubation with a selection agent): No data
- Fixation time (start of exposure up to fixation or harvest of cells): No data

SELECTION AGENT (mutation assays): No data
SPINDLE INHIBITOR (cytogenetic assays): No data
STAIN (for cytogenetic assays): No data

NUMBER OF REPLICATIONS: Two

NUMBER OF CELLS EVALUATED: No data

DETERMINATION OF CYTOTOXICITY
- Method: mitotic index; cloning efficiency; relative total growth; other: No data

OTHER EXAMINATIONS:
- Determination of polyploidy: No data
- Determination of endoreplication: No data

Rationale for test conditions:

No data

Evaluation criteria:

Dose dependent increase in number of revertants.

Statistics:

Kastenbaum and Bowman Method

Results and discussion

Test results

Species / strain:

other: Species: Salmonella typhimurium Strain: TA1535, TA100, TA1537, TA1538, TA98

Metabolic activation:

with and without

Genotoxicity:

not specified

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

not specified

Untreated negative controls validity:

not valid

Positive controls validity:

valid

Additional information on results:

No data

Remarks on result:

other: No mutagenic effect were observed

Applicant's summary and conclusion

Conclusions:

The mutagenicity potential of α-Amylcinnamaldehyde was evaluated by performing mutagenicity assay in Salmonella typhimurium strains TA1535, TA100, TA1537, TA1538, TA98, test substance did not induce mutation in all bacterial tester strains. Therefore α-Amylcinnamaldehyde was considered to be not mutagenic in Salmonella typhimurium strains.

Executive summary:

The mutagenicity effect of α-Amylcinnamaldehyde was studied by performing experiment in Salmonella typhimurium. Salmonella typhimurium stains TA1535, TA100, TA1537, TA1538, TA98 were used in study. Mutagenic assay was performed by plate incorporation method with and without metabolic activation i.e. S9 mix prepared from liver fraction of Aroclor pretreated rats (Aroclor 1254). DMSO used as solvent for test substance. Five doses of each substance were tested (up to 3.6 mg/plate) in all five tester strains Vogel Bonnet medium was used throughout; plates were incubated for 48 hr. Positive controls were run in each experiment with the reference mutagens sodium azide and benzo[a]pyrene. Test substance was tested at least twice.

Test substance did not induce mutation in the tester strains, therefore α-Amylcinnamaldehyde was considered to be not mutagenic in Salmonella typhimurium strains.