Benzenesulfonic acid, 2-amino-4,5-dichloro-

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1990

Report date:

1990

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: German standard DIN 38412 part 27

Principles of method if other than guideline:

DIN 38412 Part 27 (draft, status 1989): Determination of the inhibitory effect of waste water on the oxygen consumption of Pseudomonas putida (Pseudomonas - oxygen consumption inhibition test)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

3,4-Dichloroaniline-6-sulfonic acid

EC Number:

700-413-2

Cas Number:

6331-96-0

Molecular formula:

C6 H5 Cl2 N O3 S

IUPAC Name:

3,4-Dichloroaniline-6-sulfonic acid

Details on test material:

- Name of test material (as cited in study report): Dichlororthanilsaeure (getrocknet)- Lot/batch No.: 530/89- Analytical purity: 97.9 %

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)- Method: the test substance was added to the test water and the mixture was stirred for 17 hours- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Cremophor RH 40 (100 mg/l) was used as emulgator.

Test organisms

Test organisms (species):

Pseudomonas putida

Details on inoculum:

- Laboratory culture: The test strain of Pseudomonas putida DSM 50026 used is obtained at regular intervals from DSM (German Collection of Microorganism)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

30 min

Test conditions

Test temperature:

21 ± 1 °C

pH:

not adjusted: 2.8 - 6.7adjusted: 7.2 - 7.3

Nominal and measured concentrations:

Nominal test substance concentrations:312.5, 156.25, 78.12, 39.06 mg/lwith pH adjustment:10000, 2500, 625, 156.3 mg/l

Details on test conditions:

TEST SYSTEM- Test vessel: Erlenmeyer flask (250 ml)- Fill volume: 100 ml- No. of vessels per concentration (replicates): 1- No. of vessels per control (replicates): 4- Biomass loading rate: 240 - 400 TE/F of the sampleOTHER TEST CONDITIONS- Adjustment of pH: yes

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

30 min

Dose descriptor:

EC10

Effect conc.:

78 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 2

Duration:

30 min

Dose descriptor:

EC50

Effect conc.:

200 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 3

Duration:

30 min

Dose descriptor:

other: EC90

Effect conc.:

280 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Details on results:

The neutralized assay (10000 mg/l) showed no toxicity.

Any other information on results incl. tables

Change in oxygen consumption rate compared with blank control after 30 min (concentration in mg/l):
  ------------------------------------------
  ¦ conc. ¦ 312.5 ¦ 156.25 ¦ 78.12 ¦ 39.06 ¦
  ------------------------------------------
  ¦ rate  ¦   0 % ¦   74 % ¦  90 % ¦  97 % ¦
  ------------------------------------------