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Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1990
Report Date:
1990

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: German standard DIN 38412 part 27
Principles of method if other than guideline:
DIN 38412 Part 27 (draft, status 1989): Determination of the inhibitory effect of waste water on the oxygen consumption of Pseudomonas putida (Pseudomonas - oxygen consumption inhibition test)
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Dichlororthanilsaeure (getrocknet)- Lot/batch No.: 530/89- Analytical purity: 97.9 %

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)- Method: the test substance was added to the test water and the mixture was stirred for 17 hours- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Cremophor RH 40 (100 mg/l) was used as emulgator.

Test organisms

Test organisms (species):
Pseudomonas putida
Details on inoculum:
- Laboratory culture: The test strain of Pseudomonas putida DSM 50026 used is obtained at regular intervals from DSM (German Collection of Microorganism)

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
30 min

Test conditions

Test temperature:
21 ± 1 °C
pH:
not adjusted: 2.8 - 6.7adjusted: 7.2 - 7.3
Nominal and measured concentrations:
Nominal test substance concentrations:312.5, 156.25, 78.12, 39.06 mg/lwith pH adjustment:10000, 2500, 625, 156.3 mg/l
Details on test conditions:
TEST SYSTEM- Test vessel: Erlenmeyer flask (250 ml)- Fill volume: 100 ml- No. of vessels per concentration (replicates): 1- No. of vessels per control (replicates): 4- Biomass loading rate: 240 - 400 TE/F of the sampleOTHER TEST CONDITIONS- Adjustment of pH: yes

Results and discussion

Effect concentrationsopen allclose all
Duration:
30 min
Dose descriptor:
EC10
Effect conc.:
78 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
30 min
Dose descriptor:
EC50
Effect conc.:
200 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
30 min
Dose descriptor:
other: EC90
Effect conc.:
280 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Details on results:
The neutralized assay (10000 mg/l) showed no toxicity.

Any other information on results incl. tables

Change in oxygen consumption rate compared with blank control after 30 min (concentration in mg/l):
------------------------------------------
¦ conc. ¦ 312.5 ¦ 156.25 ¦ 78.12 ¦ 39.06 ¦
------------------------------------------
¦ rate ¦ 0 % ¦ 74 % ¦ 90 % ¦ 97 % ¦
------------------------------------------

Applicant's summary and conclusion