Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1994
Report Date:
1994

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: according to Directive 79/831/EEC, Annex V, Part C.2:
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Dichlororthanilsäure (getrocknet)- Lot/batch No.: 530/89- Analytical purity: 97.9 %

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)- Method: A stock solution (100 mg/l) was prepared. The test concentrations were prepared by serial dilution of the stock solution.

Test organisms

Test organisms (species):
other: Daphnia magna STRAUS
Details on test organisms:
TEST ORGANISM- Source: originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.- Age at study initiation (mean and range, SD): 2-24 h

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
2.2 - 2.3 mmol/l
Test temperature:
19.9 - 20.3 °C
pH:
7.8 - 8.0
Dissolved oxygen:
9.0 - 9.6 mg/l
Nominal and measured concentrations:
Nominal test substance concentrations:0, 6.25, 12.5, 25, 50 and 100 mg/l
Details on test conditions:
TEST SYSTEM- Test vessel: flat bottom glass tubes (20 ml)- Fill volume: 10 ml- No. of organisms per vessel: 5- No. of vessels per concentration (replicates): 4- No. of vessels per control (replicates): 4- Biomass loading rate: 1/2 animals per mlTEST MEDIUM / WATER PARAMETERS- Source/preparation of dilution water: synthetic test medium (M4) prepared from dionised water- Alkalinity up to pH 4.3: 0.80 - 1 .00 mmol/l- Ca/mg ratio: 4:1- Conductivity: 650 μS/cmOTHER TEST CONDITIONS- Photoperiod: 16:8 h day-night regimeEFFECT PARAMETERS MEASURED (with observation intervals if applicable) :Mobiliy after 0, 3, 6, 24 and 48 h

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Reported statistics and error estimates:
Due to the results of the test a statistical evaluation was not necessary.

Applicant's summary and conclusion