Benzenesulfonic acid, 2-amino-4,5-dichloro-

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1994

Report date:

1994

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: according to Directive 79/831/EEC, Annex V, Part C.2:

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

3,4-Dichloroaniline-6-sulfonic acid

EC Number:

700-413-2

Cas Number:

6331-96-0

Molecular formula:

C6 H5 Cl2 N O3 S

IUPAC Name:

3,4-Dichloroaniline-6-sulfonic acid

Details on test material:

- Name of test material (as cited in study report): Dichlororthanilsäure (getrocknet)- Lot/batch No.: 530/89- Analytical purity: 97.9 %

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)- Method: A stock solution (100 mg/l) was prepared. The test concentrations were prepared by serial dilution of the stock solution.

Test organisms

Test organisms (species):

other: Daphnia magna STRAUS

Details on test organisms:

TEST ORGANISM- Source: originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.- Age at study initiation (mean and range, SD): 2-24 h

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.2 - 2.3 mmol/l

Test temperature:

19.9 - 20.3 °C

pH:

7.8 - 8.0

Dissolved oxygen:

9.0 - 9.6 mg/l

Nominal and measured concentrations:

Nominal test substance concentrations:0, 6.25, 12.5, 25, 50 and 100 mg/l

Details on test conditions:

TEST SYSTEM- Test vessel: flat bottom glass tubes (20 ml)- Fill volume: 10 ml- No. of organisms per vessel: 5- No. of vessels per concentration (replicates): 4- No. of vessels per control (replicates): 4- Biomass loading rate: 1/2 animals per mlTEST MEDIUM / WATER PARAMETERS- Source/preparation of dilution water: synthetic test medium (M4) prepared from dionised water- Alkalinity up to pH 4.3: 0.80 - 1 .00 mmol/l- Ca/mg ratio: 4:1- Conductivity: 650 μS/cmOTHER TEST CONDITIONS- Photoperiod: 16:8 h day-night regimeEFFECT PARAMETERS MEASURED (with observation intervals if applicable) :Mobiliy after 0, 3, 6, 24 and 48 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Reported statistics and error estimates:

Due to the results of the test a statistical evaluation was not necessary.