Registration Dossier

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1991
Report Date:
1991

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: German standard DIN 38412 part L15
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Dichlororthanilsaeure (trocken)- Lot/batch No.: P 539/88- Analytical purity: 98.8 %

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)- Method: The product was added to the test water without any pretreatment- Evidence of undissolved material (e.g. precipitate, surface film, etc): increasing amounts of undissolved test substance accumulated at the water surface and at the bottom of the aquaria with increasing test concetrations. After pH adustment at a concentration of 1000 m/l the test substance was completely dissolved.

Test organisms

Test organisms (species):
Leuciscus idus
Details on test organisms:
TEST ORGANISM- Common name: Golden orfe- Source: Fischzucht Eggers, Hohenwestedt, Germany. - Length at study initiation (length definition, mean, range and SD): 7.0 cm (range: 6.5 - 7.8 cm)- Weight at study initiation (mean and range, SD): 3.4 g (range: 2.5 - 5.1 g)ACCLIMATION- Acclimation period: 3 days- Type and amount of food: Growing feed F/B 50, Ssniff Spezialdiäten GmbH, Soest, Germany; ad libitum - Feeding frequency: daily- Health during acclimation (any mortality observed): no mortality observed

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Hardness:
2.5 mmol/l
Test temperature:
23-24 °C
pH:
after 1 h: 7.5 (100 mg/l)3.0 (215 mg/l)2.4 (464 mg/l)2.0 (1000 mg/l)6.1 (1000 mg/l after pH-adjustment)
Dissolved oxygen:
5.1 - 7.9 mg/l
Nominal and measured concentrations:
Nominal test substance concentrations: control, 100, 215, 464, 1000 mg/l and 1000 mg/l with pH adjusment
Details on test conditions:
TEST SYSTEM- Test vessel: glass aquarium (30xX 22x24 cm)- Fill volume: 10 l- No. of organisms per vessel: 10 - No. of vessels per concentration (replicates): 1- No. of vessels per control (replicates): 1- Biomass loading rate: 3.4 g/lTEST MEDIUM / WATER PARAMETERS- Source/preparation of dilution water: Reconstituted freshwater according to DIN 38 412, part 11OTHER TEST CONDITIONS- Adjustment of pH: yes- Photoperiod: 16:8 h day-night regimeEFFECT PARAMETERS MEASURED (with observation intervals if applicable) :Mortality and symptoms of intoxication after 1, 4, 24, 48, 72 and 96 hoursTEST CONCENTRATIONS- Results used to determine the conditions for the definitive study: LC50 after 96 hours: 100-1000 mg/l

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
100 - 220 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
215 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
No mortality was observed in the 1000 mg/l group after pH-adjustment.Apathy was observed at a concentration of 215 mg/l after 4 hours of exposure.
Reported statistics and error estimates:
Finney DJ (1971) Probit Analytics. Cambridge University Press, 3rd ED

Applicant's summary and conclusion