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EC number: -
CAS number: -
Table 1: Absorption values for
negative control, test item and positive control (mean of 2
measurements, blank value substracted)
Mean – blank (Tissue 1)
Mean – blank (Tissue 2)
Mean – blank (Tissue 3)
Mean of the three Tissues
Relative Standard Deviationof the three tissues
Table 2: % Formazan production for the
test item and positive control
% Formazan production (Tissue 1)
% Formazan production (Tissue 2)
% Formazan production (Tissue 3)
% Formazan production Mean
The relative absorbance values were
reduced to 56.7% after the treatment. This value is above the threshold
for irritation (50%). Therefore, the test item is considered as not
The skin irritation potential of „2-propanol and 2-butanol
production, distn. residue“ in theHuman Skin Model Test was determined
following EU-Method B.46. One valid experiment under GLP was performed.
Three tissues of the human skin model EpiDerm(TM) were treated with
„2-propanol and 2-butanol production, distn. residue“ for 60 minutes.
30 µL of the liquid test item (using a nylon mesh) were applied to
each tissue and spread to match the tissue size. DPBS-buffer was used as
negative control, 5% SDS-solution was used as positive control. After
treatment with the negative control, the absorbance values were within
the required acceptability criterion of 1.0 < mean OD < 2.5. The
positive control showed clear irritating effects. Variation within
tissues was acceptable (< 18%).
After the treatment with the test item, the relative absorbance
values were reduced to 56.7%. This value is above the threshold for
irritation potential (50%). Therefore, „2-propanol and 2-butanol
production, distn. residue“ is considered as not irritant in the Human
Skin Model Test.
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