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EC number: -
CAS number: -
The mortalities were recorded daily
and can be found in the following table.
Table 1 Mortalities
Nom. Conc. in mg/L
Total of fish used
No abnormal behaviour of the surviving
fish was observed.
The pH values in the test media and
the control ranged from 7.8 to 8.5. The concentration of dissolved
oxygen stayed above 7.7 mg/L or 90% throughout the test. Temperature
range was 21.7 – 22.9 °C. The details are given in the following tables:
Table 2a pH values
Nominal Concentration in mg/L
pH at t in h (old/new test solution)
Table 2b O2
O2in mg/L at t in h (old/new test solution)
Table 2c Temperature
The 96h-LC50 of reaction mass of "2-propanol and 2-butanol
production, distn. residues" (main constituent 2,2'-oxybisbutane (DSBE),
DIPE, SBA and 2 -methylpropan-2 -ol (TBA)) were determined for Danio
rerio according to OECD 203 resp. EU C.1.
Due to the volatility, of the test item the toxicity against Danio
rerio was tested using a semi-static test design in closed vessels. The
main study was performed as a limit test at nominal concentration 101
mg/L (corresponding to a threshold approach concentration of 41 mg/L,
lowest EC 50 in alga and daphnia test). The necessary nominal load of
test item based on the density of the test item (0.76 g/ml), the content
of organic carbon of 76 % and recovery rates of DOC measurements was
determined in non-GLP pre-tests. Therefore, the test solutions were
prepared in higher concentrations than the threshold concentration (101
mg/L instead of 41 mg/L).
Seven fish were exposed to the test item for 96 hours in a
semi-static test design. The treatment showed no mortality.
At the beginning and every 24 hours during the test, the content
of test item was determined by calculation from content of organic
carbon of the test item (76%) and TOC-determination in the new and old
test solutions. The geometric mean of the measured concentrations was
used for the determination of the biological results.
The following results for the test item reaction "2-propanol and
2-butanol production, distn. residues" could be determined:
96h NOEC = 71 mg/L
96h LC50 > 71 mg/L
The following results were determined for the test item
"2-propanol and 2-butanol production, distn. residues" (species: Danio
rerio): 24h, 48h, 72h and 96h-NOEC = 71 mg/L; 24h, 48h, 72h and 96h-LC50
>71 mg/L; 24h, 48h, 72h and 96h-LC100 > 71 mg/L.
The treatment showed no mortality.
The LC50 value and the NOEC for the mortality of Danio rerio of
"2-propanol and 2-butanol production, distn. residues" was measured
according to OECD 203, respectively EU C.1 guideline under GLP.
Following results were obtained: 96h-NOEC = 71 mg/L; 96h-LC50 = > 71 mg/L
The measured concentrations were higher than the nominal
concentration. As no mortality occurred, this is stated as uncritical.
The analytical determinations of the test item showed good correlation
between old and new test solutions. The determination of the results was
based on the geometric mean of the concentrations.
The treatment showed no mortality to Danio rerio.
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