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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1995-03-10 to 1995-03-22
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Qualifier:
according to
Guideline:
other: DIN 38412 L31
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Qualifier:
equivalent or similar to
Guideline:
EU Method C.1 (Acute Toxicity for Fish)
GLP compliance:
not specified
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
Leuciscus idus
Details on test organisms:
TEST ORGANISM
- Common name: Gold orfe (Leuciscus idus)
- Source: Fischzucht Eggers, Postfach 12, 24594 Hohenstedt, Germany
- Length at study initiation (length definition, mean, range and SD): 5-8 cm
- Feeding during test: no
ACCLIMATION
- Acclimation period: one week quaratine
- Acclimation conditions (same as test or not): not
- Feeding frequency: daily
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
Sum of Ca and Mg ions: 2.5 mmol/L
Test temperature:
20+-1°C
pH:
6.8 to 7.2
Dissolved oxygen:
8.3 to 8.6 mg O2/L
Salinity:
not applicable
Nominal and measured concentrations:
0, 2, 3, 4, 5, 6 g/L
Details on test conditions:
TEST SYSTEM
- Test vessel: all glass aquarium (30 L)
- Water volume per vessel: 10 L
- Aeration: min. 80% from saturation value
- No. of organisms per vessel: 3 fish per concentration
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Day-Light-Cyclus: 16 h:8 h
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: test medium was prepared from reagent grade salts (Ca, Mg, Na, K) and demineralized water- Ca/Mg ratio: 4:1- Conductivity: 0.5 µS/cm
Reference substance (positive control):
no
Key result
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
2 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
- Behavioural abnormalities: no- Observations on body length and weight: 6.5 to 7.3 cm; 2.2 to 3.1 g- Mortality of control: no

Cumulative mortality


 Nominal concentration (g/L)  24 h  48 h
 control  0  0
 2  0  0
 3  2  2
 4  0  1
 5  2  2
 6  2  2
Validity criteria fulfilled:
yes
Conclusions:
The toxicological concentration limit for buscopan is 2 g/L at 20°C under the conditions of the test. In consequence, the NOEC is 2 g/L.

Description of key information

A 48-hour NOEC of 2 g/L was determined for the effects of the read-across substance Hyoscine butylbromide on mortality for fish according to an reliable study. No LC50 was determined in this study, the LC50 will be defined to be >2 g/L.

Key value for chemical safety assessment

Additional information