Registration Dossier
Registration Dossier
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EC number: 201-732-5 | CAS number: 87-22-9
Endpoint summary
Administrative data
Description of key information
Biodegradation in water:
The ready biodegradability of test chemical was determined in accordance with the OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test) (Experimental study report, 2000). Fresh activated sludge from a biological wastewater treatment plant treating predominantly domestic sewage was used. The sludge is collected in the morning, washed three times in the mineral medium (by centrifuging at 1000 g for 10 minutes, discarding the supernant and resuspending in mineral medium) and kept aerobic until being used on the same day. Initial test substance concentration used in the study was 100 mg/l. Mineral medium was prepared by mixing 50 ml of solution A and 2000 ml deionised water, adding 5 ml of each of the solutions B, C and D and making up to 5 litres with deionised water. The pH is measured and if necessary, adjusted to 7.4 +/- 0.2 with phosphoric acid or potassium hydroxide. The water used during this study is deionised water containing less than 10 mg/l dissolved organic carbon. Suspended solids concentration used in the study was 3.289 g/l. To obtain a concentration of inoculum of 30 mg/l (dry weight) in a 250 ml flask, 2.28 ml of sludge is needed (inoculum). Sodium benzoate (100 mg/l) (purity min. 99.0%) was used as a reference substance. Degradation of sodium benzoate exceeded 40% after 7 days and 65% after 14 days, the activity of the inoculum was thus verified, and the test is considered as valid. Test chemical undergoes 93% biodegradation after 28 days in the test conditions. Biodegradation starts on day 3 and reached 80% at the end of the 10-day window (day 3 to 13). The curves obtained with the reference substance alone and with test chemical plus reference substance show no toxic effect of test chemical to the micro-organisms at the test concentration (100 mg/l). Thus, test chemical is considered as readily biodegradable.
Biodegradation in water and sediment: simulation test
In accordance with column 2 of Annex IX of the REACH regulation, testing for this end pointis scientifically not necessary and does not need to be conducted since the test chemicalis readily biodegradable in water.
Biodegradation in soil
In accordance with column 2 of Annex IX of the REACH regulation, testing for this end point is scientifically not necessary and does not need to be conducted since the test chemical is readily biodegradable in water.
Additional information
Biodegradation in water:
The ready biodegradability of test chemical was determined in accordance with the OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test) (Experimental study report, 2000). Fresh activated sludge from a biological wastewater treatment plant treating predominantly domestic sewage was used. The sludge is collected in the morning, washed three times in the mineral medium (by centrifuging at 1000 g for 10 minutes, discarding the supernant and resuspending in mineral medium) and kept aerobic until being used on the same day. Initial test substance concentration used in the study was 100 mg/l. Mineral medium was prepared by mixing 50 ml of solution A and 2000 ml deionised water, adding 5 ml of each of the solutions B, C and D and making up to 5 litres with deionised water. The pH is measured and if necessary, adjusted to 7.4 +/- 0.2 with phosphoric acid or potassium hydroxide. The water used during this study is deionised water containing less than 10 mg/l dissolved organic carbon. Suspended solids concentration used in the study was 3.289 g/l. To obtain a concentration of inoculum of 30 mg/l (dry weight) in a 250 ml flask, 2.28 ml of sludge is needed (inoculum). Sodium benzoate (100 mg/l) (purity min. 99.0%) was used as a reference substance. Degradation of sodium benzoate exceeded 40% after 7 days and 65% after 14 days, the activity of the inoculum was thus verified, and the test is considered as valid. Test chemical undergoes 93% biodegradation after 28 days in the test conditions. Biodegradation starts on day 3 and reached 80% at the end of the 10-day window (day 3 to 13). The curves obtained with the reference substance alone and with test chemical plus reference substance show no toxic effect of test chemical to the micro-organisms at the test concentration (100 mg/l). Thus, test chemical is considered as readily biodegradable.
Biodegradation in water and sediment: simulation test
In accordance with column 2 of Annex IX of the REACH regulation, testing for this end pointis scientifically not necessary and does not need to be conducted since the test chemicalis readily biodegradable in water.
Biodegradation in soil
In accordance with column 2 of Annex IX of the REACH regulation, testing for this end point is scientifically not necessary and does not need to be conducted since the test chemical is readily biodegradable in water.
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