o-phenylenediacetonitrile

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1997

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method B.1 (Acute Toxicity (Oral))

GLP compliance:

yes (incl. QA statement)

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

o-phenylenediacetonitrile

EC Number:

210-351-3

EC Name:

o-phenylenediacetonitrile

Cas Number:

613-73-0

Molecular formula:

C10H8N2

IUPAC Name:

2-[2-(cyanomethyl)phenyl]acetonitrile

Test material form:

solid: particulate/powder

Test animals

Species:

rat

Strain:

Wistar

Sex:

male

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

not specified

Results and discussion

Effect levels

Effect levels 1

Sex:

male

Dose descriptor:

LD50

Effect level:

ca. 642 mg/kg bw

Based on:

test mat.

Effect levels 2

Key result

Sex:

female

Dose descriptor:

LD50

Effect level:

ca. 390 mg/kg bw

Based on:

test mat.

Applicant's summary and conclusion

Interpretation of results:

Category 4 based on GHS criteria

Conclusions:

Harmful if swallowed

Executive summary:

Acute oral toxicity of lr2 Phenylendiacetonitril was tested in female and male Charles River

Wistar rats. The test substance was administered in doses between 800 and 200 mglkg body

weight (bw) by stomach tube.

The observation period was 14 days long.

Animals were examined for mortality, clinical signs, body weight gain and pathological

alterations of organs.

The LD50value was for male animals 642 mglkg bw and for

female animals 390 mg /kg bw.

The animals died in the most cases in the first 4 hours p.a.

The animals responded very quickly to the application of the test substance. The period of

recovery of the survival animals was finished on day 1 p.a.

Moderate to severe clinical signs were observed in all animals treated with the test substance.

The most striking findings were apathy, squatting/abdominal position, disorder of respiration in

rhythm or intensþ and tremor and clonic convulsions. A dose independent increased blood

circulation of the skin was observed in nearly all animals.

The body weight development was dose dependent retarded in the surviving animals.

The macroscopic pathological examination showed haemorrhagic gastric mucosa and

pulmonary emphysema in the animals which died in the course of testing.

The pulmonary emphysema could be seen as cause of death.

The tremor and convulsions or the increased circulation show the central action of the test

substance.

According to the criteria of Annex IV of Council Directive 93/21/EEC Commission Directive

of 2TthApril 1993 1,2 Phenylendiacetonitril is after the examination of acute oral toxicþ a

harmful substance and is to label with R 22 "Harmful if swaltowed" and the danger symbol