Xanthylium, 9-[2-(ethoxycarbonyl)phenyl]-3,6-bis(ethylamino)-2,7-dimethyl-, molybdatesilicate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

25th june 2002 - 27th june 2002

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods

Qualifier:

equivalent or similar to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

not specified explicitly in text

GLP compliance:

not specified

Analytical monitoring:

no

Details on sampling:

- Concentrations: 0 and 100 mg/L

Vehicle:

yes

Details on test solutions:

prepared in ISO , prepared in milli-RO water

Test organisms (species):

Daphnia magna

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

not specified

Test temperature:

19.9°C

pH:

7.7 - 8.0

Dissolved oxygen:

7.8 - 9.1 mg/L

Salinity:

not specified

Conductivity:

not specified

Nominal and measured concentrations:

nominal 0 and 100 mg/L

Details on test conditions:

TEST SYSTEM
- Type (delete if not applicable): closed
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

Reference substance (positive control):

not specified

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

one daphnid observed trapped at the surface of the test solutions. these daphnids were re-immersed in the respective solutions before scoring of mobility

Results with reference substance (positive control):

not specified

Reported statistics and error estimates:

not specified

Validity criteria fulfilled:

not specified

Conclusions:

Under the conditions of the the study, 8135C did not induce acute immobilisation of daphnia magna in a WAF loaded at 100 mg/L after 48 hours of exposure. The 48h EC50 was > a WAF loaded at 100mg/L.

Reference 2

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

24th june 2003 - 26th june 2003

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

not specified

Specific details on test material used for the study:

Pigment red 81:5/4 (silicomolybdic acid salt of C.I. 45160:4)
Red powder
Batch: pigment red 81:5/4

Analytical monitoring:

no

Details on sampling:

- Concentrations: 0.01, 0.1, 1, 10 mg/L

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: stock solutions were prepared in acetone at a concetration of a factor of 10,000 higher than the final test solutions
- Eluate: 0.1mL of the stock solution was added per litre of test medium
- Differential loading: water accommodated fractions
- Controls: contain same amount of solvent as test item

Test organisms (species):

Daphnia magna

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

not specified

Test temperature:

20.2 - 21.0°C

pH:

7.6 - 7.8

Dissolved oxygen:

remained above 7mg/L

Salinity:

not specified

Conductivity:

not specified

Nominal and measured concentrations:

nominal 0.01, 0.1, 1, 10 mg/L

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

< 0.01 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Results with reference substance (positive control):

none specified

Validity criteria fulfilled:

not specified

Conclusions:

The 48h EC50 was below 0.01mg/L
According to EEc directive 98/98, pigment red 81:5/4 should be classified as highly toxic to aquatic organisms

Reference 3

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

24th june 2003 - 26th june 2003

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

not specified

Specific details on test material used for the study:

pigment red 81:5/3
silicomolybdic acid salt of C.I 45160:4

Analytical monitoring:

no

Details on sampling:

concentrations: 0.01, 0.1, 1, 10 mg/L

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: stock solutions prepared in acetone
- Eluate: water acommodated fractions
- Controls: yes, concurrent without test item

Test organisms (species):

Daphnia magna

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

not specified

Test temperature:

19.3 - 20.6°C

pH:

7.7 - 7.9

Dissolved oxygen:

remained above 7 mg/L

Salinity:

not specified

Conductivity:

not specified

Nominal and measured concentrations:

nominal: 0.01, 0.1, 1, 10 mg/L

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

< 0.01 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Results with reference substance (positive control):

none specified

Reported statistics and error estimates:

not specified

Validity criteria fulfilled:

not specified

Conclusions:

In conclusion the 48h EC50 was below 0.01 mg/L.
According to EEC directive 98/98, pigment red 81:5/3 should be classified as highly toxic to aquatic organisms

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

1 mg/L

Additional information

EL50 value taken from the long term toxicity study to aquatic invertebrates (BQ63Y)