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Short-term toxicity to fish

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Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
From November 23 to 27, 1987
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods with acceptable restrictions
Qualifier:
according to
Guideline:
other: DIN 38 412 "TESTVERFAHREN MIT WASSERORGANISMEN (GRUPPE L). ALLGEMEINE HINWEISE ZUR PLANUNG, DURCHFUEHRUNG UND AUSWERTUNG BIOLOGISCHER TESTVERFAHREN (Ll)"
Deviations:
not specified
Qualifier:
according to
Guideline:
other: "BESTIMMUNG DER WIRKUNG VON WASSERINHALTSSTOFFEN AUF FISCHE - FISCHTEST (L15)", JUNE 1982.
Deviations:
not specified
GLP compliance:
no
Details on sampling:
- Concentrations: 10.0, 21.5, 46.4 and 100 mg/l.
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Preparation of the test substance: The product was added to the test water without any pretreatment; subsequently the fish were placed into the aquaria.
- Reasons for the selection of the concentrations: based on the results of 2 range finding studies and 1 main study not evaluated (LC50 after 96 h: between 32 and 100 mg/l).
Test organisms (species):
other: Leuciscus idus l., golden variety
Details on test organisms:
TEST ORGANISM
- Common name: Golden Orfe
- Strain: Leuciscus Idus L., Golden Variety
- Source: Fischzucht Paul Eggers D-2354 Hohenwestedt, Frg
- Length at study initiation: 5.3 Cm (Range: 4.7 - 5.7)
- Weight at study initiation: 3.7 G (Range: 2.1 - 4.9)
- Food type: Growing Feed F/B 50 Ssniff Spezialdiaeten Gmbh D-4770 Soest, Frg ad Libitum

ACCLIMATION
- Acclimation period: about 8 Weeks

OTHER CONDITIONS
- Water Temperature: 20 Centigrade +-1
- Medical Treatment: Twice With 0.05 Mg/L Malachite Green Chloride
- Housing: The fish were kept in a flow-through tank in tap water cleaned by active carbon and aerated with oil-free air.
- Mortality during the last 2 weeks of housing: about 0.2%
- Mortality during the adaptation period: 0%
- Medical Treatment: twice with 0.05 mg/l malachite green chloride once with 10 mg/l tetracycline hydrochloride


Details on test conditions:
TEST SYSTEM
- Test vessel: All-glass aquarium (30 cm x 22 cm x 24 cm)
- Aeration: continuous aeration with oil-free air
- No. of organisms per test concentrations: 10
- Loading (g fish / l test water): 3.7
- Adaptation to test water and test temperature: 3 days
- Withdrawel of food before exposure: 1 day

TEST MEDIUM / WATER PARAMETERS
- Test Water: reconstituted freshwater according to din 38 412, part 11, draft sept. 198].preparation from fully demineralized tap water conductivity: max. 10 micro mho
- Total Hardness : about 2.5 mmol/l
- Acid Capacity : about 5.5 mmol/l
- Ratio Ca/Mg Ions : 4 :1
- Ratio Na/K Ions : 10: 1
- Oxygen Content : > 60% of maximum saturation
- Test Medium:
294.0 mg/l CaCl2 . 2 H2O
123.3 mg/l MgSO4 .7 H2O
64.8 mg/l NaHCO3
5.8 mg/l KCl

OTHER TEST CONDITIONS
- pH : about 8.0
- Photoperiod: 16 hours light and 8 hours dark


Reference substance (positive control):
yes
Remarks:
Chloroacetamide
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 46.4 - < 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Results with reference substance (positive control):
LC50 (48 h)= about 26 mg/l
Validity criteria fulfilled:
not specified
Conclusions:
46.4 mg/l
Executive summary:

Method

The short-term toxicity to fish was determined according to DIN 38 412 "Testverfahren mit wasserorganismen (gruppe l). allgemeine hinweise zur planung, durchfuehrung und auswertung biologischer testverfahren (ll)". The test was conducted at four different concentration: 10.0, 21.5, 46.4 and 100 mg/l.

Conclusion

46.4 mg/l <LC50< 100 mg/l

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
March 25, 1976
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Qualifier:
no guideline available
Principles of method if other than guideline:
Internal Method 11
GLP compliance:
no
Test organisms (species):
other: Trout
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
45 mg/L
Nominal / measured:
not specified
Conc. based on:
not specified
Basis for effect:
not specified
Validity criteria fulfilled:
not specified
Conclusions:
LC50 = 45 mg/l
Executive summary:

Only short abstarct is available. The LC50 value calculated in this test was 45 mg/l.

Description of key information

46.4 mg/l <LC50< 100 mg/l

Key value for chemical safety assessment

Additional information

The short-term toxicity to fish of the test substance was determined according to DIN 38 412 "Testverfahren mit wasserorganismen (gruppe l). allgemeine hinweise zur planung, durchfuehrung und auswertung biologischer testverfahren (ll)". The test was conducted at four different concentration: 10.0, 21.5, 46.4 and 100 mg/l. The LC50 value was found to be in a range of 46.4 mg/l and 100 mg/l.

This result is in line with a further test where the LC50 value calculated was 45 mg/l.