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Ecotoxicological information

Toxicity to aquatic plants other than algae

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Administrative data

Endpoint:
toxicity to aquatic plants other than algae
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Peer-reviewed technical publication that meets basic scientific principles.

Data source

Reference
Reference Type:
publication
Title:
Toxicity Tests of Aquatic Pollutants by Using Common Duckweeed
Author:
Wang, W
Year:
1986
Bibliographic source:
Environmental Pollution (Series B) 11 (1986) 1-14

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Six different concentrations of boron were added to glass jars with duckweed and growth was recorded.
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Boric acid
EC Number:
233-139-2
EC Name:
Boric acid
Cas Number:
10043-35-3
Molecular formula:
H3BO3
IUPAC Name:
Boric acid
Details on test material:
Chemical test compounds were reagent grade.

Sampling and analysis

Analytical monitoring:
not specified

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Lemna minor
Details on test organisms:
TEST ORGANISM
- Common name: Duckweed
- Source (laboratory, culture collection): collected from a pond located inside the Illinois State Water Survey property during the spring of 1982
- Method of cultivation: The stock was maintained in a polypropylene tray with 20 litre capacity. To the tray was added a small amount of sediment, collected from the same pond, 10 litres of pond water and 5 litres of cold tap water. Every 3-4 days cold tap water was added to maintain the water level.
- 24 h before testing duckweed samples were selected for tests. The selected specimens must appear healthy, with 2 fronds of +- equal size per colony

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
7 d

Test conditions

Test temperature:
27 +- 2 °C
pH:
7.5
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 200 ml fruit jars, to each jar was added a toxicant and 200 ml of plant nutrient solution
- No. of colonies per vessel: 20
- No. of fronds per colony: 2
- No. of vessels per concentration (replicates): 3 - 6
- No. of vessels per control (replicates): 3 - 6


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: nutrient solution prepared according to Standard Methods (1980) by using deionised water


OTHER TEST CONDITIONS
- Light intensity and quality: cool-white fluorescent light with an intensity of 6456 lux


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of frond number: manual counting

Results and discussion

Effect concentrations
Duration:
7 d
Dose descriptor:
NOEC
Effect conc.:
>= 60 mg/L
Nominal / measured:
nominal
Conc. based on:
element
Basis for effect:
frond number

Any other information on results incl. tables

7d EC50 growth >60 mg B/L

Applicant's summary and conclusion