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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
other: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Study period:
From August 03 to 23, 1990
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Remarks:
The test was conducted by means of Read Across approach. The reliability of the source study report is 1. Further information was attached at section 13
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
no
Qualifier:
according to
Guideline:
other: Acute Toxicity Test for Freshwater Invertebrates, EPA- 540/9-85-005, June, 1985.
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
yes
Details on sampling:
Samples with a nominal concentration of 100 mg/l of the substance were diluted 10 fold with test medium prior to the analysis. The stock solution of the biological test was diluted 40 fold with test medium. All other samples were analysed by VIS-spectroscopy without any dilution.
Vehicle:
no
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Source: bred in the laboratories of RCC under standardized conditions
- Age at study initiation: (6-24 hours old)
- Feeding during test: NO
- Method of breeding: standardized conditions

ACCLIMATION
- Acclimation period:24 hours
- Temperature: 18-22 °C with a max. deviation of ± 1 °C during the experiment
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
2.5 mmol/l as CaCO3
Test temperature:
19.0 - 20.5 °C
pH:
7.9
Dissolved oxygen:
8.5 ± 0.2 mgO2/l
Nominal and measured concentrations:
nominal test concentrations: 15.6, 62.5 and 250 mg test article/l
Details on test conditions:
TEST SYSTEM
- Test vessel: 100 ml glass baker
- Type: closed
- Material, size, headspace, fill volume: 50 ml
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration: 4
- No. of vessels per control: 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
CaCl2 x 2H20 294 mg/l
MgSO* x 7H20 123 mg/l
NaHCO3 65 mg/l
KCl 5.8 mg/l

- Alkalinity: 0.8 mmol/l
- Ratio Ca: Mg ratio: 4:1
- Ratio Na: K ratio: 10:1
- Conductivity: < 0.1 µs/cm

OTHER TEST CONDITIONS
- Adjustment of pH: yes, if necessary , the pH of the test medium samples for the analyses will be adjusted to pH 3-4 with hydrochloric acid
- Photoperiod: 16 hours/day
- Light intensity: 200 - 600 Lux

Reference substance (positive control):
yes
Remarks:
potassium dichromate
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
< 15 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
ca. 55 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 38.5 to 78.6 mg/L
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
ca. 250 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Validity criteria fulfilled:
yes
Conclusions:
EC50 (48h) = 55 mg/l
Executive summary:

The acute toxicity to Daphnia magna was determined in a 48-hour static test according to the OECD Guideline for Testing of Chemicals, Section 2, No. 202, adopted 4 April 84, the EEC Directive 84/449, L 251, Part C 2, and to the Guideline of the U.S. Environmental Protection Agency, Pesticide Assessment Guidelines, Subdivision E, EPA-540/9-85-005, June, 1985.

The test was performed in compliance with the Good Laboratory Practice Regulations. The nominal concentrations tested was 15.6, 31.3, 62.5, 125 and 250 mg test article/l and a control without any additions.

Test results:

24h EC 0: 31.3 mg test article/l

24h EC 50: 164.9 mg test article/l (95 % conf. limits 143.6 - 189.5 mg test article/l)

24h EC 100: 250.0 mg test article/l

48h EC 0 < 15.6 mg test article/l

48h EC 50: 55.0 mg test article/l (95 % Conf. limits: 38.5 - 78.6 mg test article/l)

48h EC 100: 250.0 mg test article/l

All reported results are related to nominal concentrations of the test article. During test duration the active ingredient concentrations were in the range of 102.6 - 110.6 % of the nominal values, under test conditions the test article was sufficiently stable.

Description of key information

EC50(48h) = 55 mg/l

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
55 mg/L

Additional information