Reaction products of acetic acid with formaldehyde and a mixture of 1-, 2-, 3-,octene

Skin sensitisation

Administrative data

Endpoint:

skin sensitisation: in vitro

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

March 2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 442D (In Vitro Skin Sensitisation: ARE-Nrf2 Luciferase Test Method)

Deviations:

no

Principles of method if other than guideline:

Although the in vitro test was not carried out in a registered GLP laboratory, the Givaudan in vitro Technologies laboratory based at it's Dubendorf site in Switzerland performs all in vitro studies within the "spirit" of GLP. In addition this Givaudan laboratory at Dubendorf devised the KeratinoSens testing protocol and instigated the assay acceptance with ECHA and the OECD. The Givaudan laboratory at Dubendorf also was a member of the DPRA acceptance testing program of laboratories and has over 4 years experience with both the KeratinoSens and DPRA assays.

GLP compliance:

no

Remarks:

Although the in vitro test was not carried out in a registered GLP laboratory, the Givaudan in vitro Technologies laboratory based at it's Dubendorf site in Switzerland performs all in vitro studies within the "spirit" of GLP.

Type of study:

activation of keratinocytes

Justification for non-LLNA method:

A combination of two in-vitro skin sensitization tests is considered to be sufficient to conclude on the skin sensitization potential of Jasmonyl LG.

Test material

Test material information

Constituent 1

Reference substance name:

Tetrahydro-3-pentyl-2H-pyran-4-yl acetate

EC Number:

242-640-5

EC Name:

Tetrahydro-3-pentyl-2H-pyran-4-yl acetate

Cas Number:

18871-14-2

Molecular formula:

C12H22O3

IUPAC Name:

3-O-acetyl-1,5-anhydro-2,4-dideoxy-2-pentylpentitol

Constituent 2

Reference substance name:

3-butyltetrahydro-5-methyl-2H-pyran-4-yl acetate

EC Number:

253-863-2

EC Name:

3-butyltetrahydro-5-methyl-2H-pyran-4-yl acetate

Cas Number:

38285-49-3

Molecular formula:

C12H22O3

IUPAC Name:

3-O-acetyl-1,5-anhydro-2-butyl-2,4-dideoxy-4-methylpentitol

impurity 1

Reference substance name:

3-pentyltetrahydro-2H-pyran-4-ol

Cas Number:

24646-98-8

Molecular formula:

C10H20O2=

IUPAC Name:

3-pentyltetrahydro-2H-pyran-4-ol

impurity 2

Reference substance name:

Likely acetylated constituents, MW 214

Molecular formula:

n/a

IUPAC Name:

Likely acetylated constituents, MW 214

impurity 3

Reference substance name:

Likely acetylated constituents

Molecular formula:

n/a

IUPAC Name:

Likely acetylated constituents

impurity 4

Reference substance name:

Unknown constituent, MW 172

Molecular formula:

n/a

IUPAC Name:

Unknown constituent, MW 172

Test material form:

liquid

Details on test material:

UVCB substance

Specific details on test material used for the study:

Batch number: PE00138468

In vitro test system

Details on the study design:

The KeratinoSensTM assay is a cell-based assay with a reporter cell line to detect potential skin sensitizers by their ability to induce the Nrf2-response.
This assay has been validated for a broad range of low-molecular weight chemicals and it was found to respond to skin sensitizers from a broad range of so called applicability domains, i.e. chemicals reacting with proteins by different mechanisms. It was validated by ECVAM and proposed to be used as part of an integrated approach for testing and assessment (IATA).

Results and discussion

Positive control results:

Postiive control: Cinnamic aldehyde

In vitro / in chemico

Results

Key result

Value:

1

Vehicle controls validity:

valid

Negative controls validity:

valid

Positive controls validity:

valid

Remarks on result:

no indication of skin sensitisation

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Executive summary:

In all three repetitions, no induction of the luciferase above the threshold of 1.5 was noted. According to the prediction model of the KeratinoSens™ assay, the test substance is rated as non-sensitizer. This conclusion is also clearly supported by the analysis of the dose-response curve in Figure 4 with overall no induction of the luciferase reporter gene to be observed.