Registration Dossier

Toxicological information

Genetic toxicity: in vitro

Currently viewing:

Administrative data

Endpoint:
in vitro cytogenicity / chromosome aberration study in mammalian cells
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
2000

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 473 (In Vitro Mammalian Chromosome Aberration Test)
Principles of method if other than guideline:
A chromosomal aberration test in CHL/IU Cells was carried out in doses up to 2.5 mg/ml.
GLP compliance:
yes
Type of assay:
in vitro mammalian chromosome aberration test

Test material

Constituent 1
Chemical structure
Reference substance name:
1,4-dihydroxyanthraquinone
EC Number:
201-368-7
EC Name:
1,4-dihydroxyanthraquinone
Cas Number:
81-64-1
Molecular formula:
C14H8O4
IUPAC Name:
1,4-dihydroxyanthraquinone
Details on test material:
Purity: 98.1%
supplier: Wako Jyunyaku Kogyo Co., Ltd.

Method

Target gene:
no data
Species / strain
Species / strain / cell type:
mammalian cell line, other: CHL/IU cells
Additional strain / cell type characteristics:
not specified
Metabolic activation:
with and without
Metabolic activation system:
S9 mix
Test concentrations with justification for top dose:
short treatment: 0.31, 0.63, 1.3, 2.5 mg/ml
continuous treatment: 0.0013, 0.0025, 0.0050, 0.010 mg/ml
Controls
Untreated negative controls:
yes
Negative solvent / vehicle controls:
yes
True negative controls:
not specified
Positive controls:
yes
Positive control substance:
benzo(a)pyrene
mitomycin C

Results and discussion

Test results
Key result
Species / strain:
mammalian cell line, other: CHL/IU cells
Metabolic activation:
with and without
Genotoxicity:
positive
Cytotoxicity / choice of top concentrations:
no cytotoxicity
Vehicle controls validity:
valid
Untreated negative controls validity:
valid
Positive controls validity:
valid

Any other information on results incl. tables

Quinizarine was positive in this CHL/IU test with and without metabolic activation.

Applicant's summary and conclusion

Conclusions:
Interpretation of results: positive
Executive summary:

A chromosomal aberration test in CHL/IU cells was carried out in doses up to 2.5 mg/ml. Quinizarine was positive in this CHL/IU test with and without metabolic activation.