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Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
28 day biodegradation test (toxicity control)
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Justification for type of information:
Although a 'guideline' study was used, the purpose of the study was to examine biodegradation.
A toxicity control group provided evidence that there is not microbial inhibition at concentrations in excess of water solubility

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2013
Report date:
2013

Materials and methods

Principles of method if other than guideline:
Evaluated as part of OECD 301 Ready Biodegradation study
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
1,4-dihydroxyanthraquinone
EC Number:
201-368-7
EC Name:
1,4-dihydroxyanthraquinone
Cas Number:
81-64-1
Molecular formula:
C14H8O4
IUPAC Name:
1,4-dihydroxyanthraquinone
Test material form:
not specified
Details on test material:
1,4-dihydroxyanthraquinone was supplied by Aldrich

Test solutions

Details on test solutions:
Tested at 25 mg/l as dispersion
Note that solublity in water is < 0.1 mg/l

Test organisms

Test organisms (species):
activated sludge of a predominantly domestic sewage

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
28 d
Remarks on exposure duration:
Tested over the duration of the biodegradation test

Test conditions

Test temperature:
22 C
pH:
Typically 7 - 8

Results and discussion

Effect concentrations
Key result
Duration:
28 d
Dose descriptor:
EC0
Effect conc.:
> 25 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The absence of inhibition at concentrations in excess of solublity is considered an indication of low microbial toxicity.