2-methylanisole

Endpoint summary

Description of key information

Short-term toxicity to fish:

Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for 2-Methylanisole (578-58-5) on Zebra Fish Danio rerio was determine to be 50 mg/L.  

Short-term toxicity to aquatic invertebrates

Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] for 2-Methylanisole (578-58-5) on test daphnia was found to be > 100 mg/L.

Toxicity to aquatic algae and cyanobacteria

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the short term toxicity on aquatic algae predicted for 1-methoxy-2-methylbenzene (CAS: 578-58-5). Effect concentration i.e EC50 value estimated to be 131.91 mg/l for Pseudokirchneriella subcapitata for 48 hrs duration. It can be concluded that the 1-methoxy-2-methylbenzene (CAS: 578-58-5)  IS likely to be not toxic to aquatic algae, hence it can be considered to be “not classified” as per the CLP classification criteria for aquatic environment.

Additional information

Short-term toxicity to fish:

Based on the experimental study whcih was conducted to assess the effect of test chemical 2-Methylanisole (578-58-5) on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was partially soluble in water. Therefore, the stock solution will be prepared by dissolving 1 g of the test substance in 1 liter of potable water (passed through reverse osmosis system) with continuous 4 hour stirring.This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item 2-Methylanisole (578-58-5) to various nominal test concentrations, LC50 was determine to be 50 mg/l . Based on the LC50, it can be consider that the chemical was hazardous and can be consider to be classified aq aquatic chronic 3 as per the CLP classification criteria.

Short-term toxicity to aquatic invertebrates :

Based on the experimental study report,Daphnia sp., Acute Immobilization Test according to OECD Guideline 203 was conducted for 2-Methylanisole (578-58-5). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media. Achieving test concentrations of 1 g/L, respectively.The nominal concentration selected for the experiment were 6.25mg/L,12.5mg/L,25mg/L,50mg/L,100mg/L and test Daphnia magna were exposed to these concentration for 48 hours. The median lethal concentration (EC50) for 2-Methylanisole (578-58-5) on Daphnia magna in a 48 hours study on the basis of immobilization effect was found to be > 100 mg/L. Thus, on the basis of this EC50 value and according to CLP criteria for aquatic classification of the substance, it is concluded that the substance, 2-Methylanisole (578-58-5) does not exhibit short term toxicity to Daphnia. EC50 (48 hours) Experimental is > 100 mg/L.

After 48 hours of exposure to test item2-Methylanisole (578-58-5)to various nominal test concentrations, EC50 was determine to be >100 mg/l . Based on the EC50, it can be consider that the chemical was non hazardous and can be consider to be not classified as per the CLP classification criteria.

Toxicity to aquatic algae and cyanobacteria :

Following studies include the predicted data and experimental study for the target chemical and structurally similar read across to conclude the 1-methoxy-2-methylbenzene (CAS: 578-58-5) toxicity towards aquatic algae is summarized as follows:

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the short term toxicity on aquatic algae predicted for 1-methoxy-2-methylbenzene (CAS: 578-58-5). Effect concentration i.e EC50 value estimated to be 131.91 mg/l for Pseudokirchneriella subcapitata for 48 hrs duration. It can be concluded that the 1-methoxy-2-methylbenzene (CAS: 578-58-5)  IS likely to be not toxic to aquatic algae, hence it can be considered to be “not classified” as per the CLP classification criteria for aquatic environment.

The above predicted data of target chemical is supported by the experimental study of structurally similar read across 2-methylpropan-1-ol(CAS: 78-83-1) from publicationWater Research 1990, suggests thatIn the Scenedesmus cell multiplication inhibition test, hazardous substances were examined to determine the effect concentrations (EC). The tests were conducted in accordance with the test procedure DIN 38 412, Part 9 (draft standard). The green alga Scenedesmus subspicatus CHODAT was cultivated as the test organism. Decreasing trend of Population changes effect were measured. The Effective concentration EC50 to 50% of Scenedesmus subspicatus is 230 mg/l. It can be concluded that the 2-methylpropan-1-ol is not toxic to the aquatic ALGAE .

Similar experimental study of another structurally similar read acrossHexan-1-ol (CAS: 111-27-3) from publicationSAR and QSAR in Environmental Research 1980,indicates that the Toxicity to aquatic algae and cyanobacteria test was carried out for 2 mins to study the effects of Hexan-1-ol in aquatic algae. The conductivity of samples 1 and 2 was measured and used to derive an ion retention health index. This index reflects the proportion of the total leechable ions present in the thallus which were retained after exposure to distilled water for 2 mins. The effective concentration EC50 TO 50% of Enteromorpha intestinalis in 2 mins is 400 mg/l. It can be concluded from the value that the Hexan-1-ol is not toxic to the aquatic algae.

Thus based on the effect concentrations which is in the range 131.91 mg/l to 400 mg/l give the conclusion that test substance 1-methoxy-2-methylbenzene (CAS: 578-58-5) was likely to be not toxic to aquatic algae at environmentally relevant concentrations and applying weight of evidence approach it can be considered to be“not classified”as per the CLP classification criteria.