Violanthrene-5,10-dione

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

14 - 18 Jan 1991

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1991

Report date:

1991

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: DIN 38412/L15

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Violanthrene-5,10-dione

EC Number:

204-152-0

EC Name:

Violanthrene-5,10-dione

Cas Number:

116-71-2

Molecular formula:

C34H16O2

IUPAC Name:

violanthrene-5,10-dione

Test material form:

solid: particulate/powder

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Leuciscus idus melanotus

Details on test organisms:

TEST ORGANISM
- Common name: golden orfe
- Source: Fischtzucht Eggers, Hohenweststedt, Germany
- Age at study initiation (mean and range, SD): consigned on 13 Nov 1990
- Length at study initiation (length definition, mean, range and SD): 7.4 cm (6.7 - 8.4 cm)
- Weight at study initiation (mean and range, SD): 4.0 g (3.0 - 5.5 g)

ACCLIMATION
- Acclimation period: 2 months
- Acclimation conditions (same as test or not): same
- Health during acclimation (any mortality observed): 0.06% mortality during last two weeks of housing

FEEDING DURING TEST
- Food type: no feeding during test

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Hardness:

2.5 mmol/L

Test temperature:

20°C

pH:

7.7 - 9.1

Dissolved oxygen:

7.5 - 8.6 mg/L

Nominal and measured concentrations:

10000 mg/L (nominal)

Details on test conditions:

TEST SYSTEM
- Test vessel: glass aquarium
- Material, size, headspace, fill volume: 30x22x24 cm, 10 L
- Aeration: slight
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 4 g/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: demineralized tap water, resalted to reconstituted water according to DIN 38412/11
- Ca/mg ratio: 4:1
- Culture medium different from test medium: no

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 light / 8 darkness

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality, sublethal symptoms

TEST CONCENTRATIONS
- Range finding study: yes
- Test concentrations: 1000 mg/L
- Results used to determine the conditions for the definitive study: yes (mortality 1/5)

Reference substance (positive control):

yes

Remarks:

chloroacetamide

Results and discussion

Effect concentrations

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: none
- Observations on body length and weight: none
- Other biological observations: none
- Mortality of control: 0%
- Other adverse effects control: 1 fish died after 24 h

Results with reference substance (positive control):

48-h LC50: 32 mg/L (1991-03-25)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 96-h LC50 was determined to be >10000 mg/L. The test substance is not acutely toxic to fish.