2,2,4(or 2,4,4)-trimethyladipic acid

Toxicity to terrestrial plants

Administrative data

Endpoint:

toxicity to terrestrial plants: short-term

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1994-12-06 to 1994-12-23

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 208 (Terrestrial Plants Test: Seedling Emergence and Seedling Growth Test)

Version / remarks:

(1984)

Deviations:

not specified

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2,2,4(or 2,4,4)-trimethyladipic acid

EC Number:

258-556-7

EC Name:

2,2,4(or 2,4,4)-trimethyladipic acid

Cas Number:

53445-37-7

Molecular formula:

C9H16O4

IUPAC Name:

2,2,4-trimethylhexanedioic acid; 2,4,4-trimethylhexanedioic acid

Details on test material:

2,2,4(or 2,4,4)-Trimethyladipic acid of Hüls AG, produced 25 March 1992. Sample from continuous production, storage tank FA 975.
Purity 90 % w/w, sample ID 3630/81389, sample No. 1324/920707

Sampling and analysis

Analytical monitoring:

no

Test substrate

Vehicle:

not specified

Details on preparation and application of test substrate:

Preparation of test medium: 0.178 g test substance + 20 g quartz sand +  1580 g soil mixed thoroughly in a plastic bag

Test organisms

1

Species:

Brassica alba

Plant group:

Dicotyledonae (dicots)

Details on test organisms:

no further information

2

Species:

Lepidum sativum

Plant group:

Dicotyledonae (dicots)

Details on test organisms:

no further information

3

Species:

Triticum aestivum

Plant group:

Monocotyledonae (monocots)

Details on test organisms:

no further information

Study design

Test type:

seedling emergence toxicity test

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

yes

Total exposure duration:

14 d

Test conditions

Test temperature:

23-25 °C

pH:

6.0 (control) / 6.8 (test)

Details on test conditions:

SOIL CHARACTERISTICS
- Commercial substrate of LUFA (Speyer, Germany),  properties:
- Sieved at 0.5 cm mesh width
- < 1.5 % organic carbon
- 10-20 % particles < 20 µm
- pH value 5-7.5
- water storage capacity 35.3 g / 100 g

TEST SYSTEM
- Culturing apparatus: Black plastic flower pots, height approx. 6 cm,  diameter approx. 8 cm (top) / approx. 6 cm (bottom), filled with 100 g  dry 
test medium
- Number of replicates: 4 replicates with 5 seeds each
- Addition of water: Initially approx. 60 % of capacity, once during  study as required
- Atmospheric humidity: Average 92 % - Photoperiod: 16 hours light (1200-2000 Lux) / 8 hours dark (50-100 Lux)

TEST PARAMETERS
- Emergence: Number of plants / number of seeds - Growth: Mean weight of plant parts above the soil surface as determined  directly at end of study

Nominal and measured concentrations:

100 mg/kg soil dry weight (nominal)

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Effect concentrations 1

Species:

Brassica alba

Duration:

17 d

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Remarks:

and growth

Effect concentrations 2

Species:

Lepidum sativum

Duration:

17 d

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Remarks:

and growth

Effect concentrations 3

Species:

Triticum aestivum

Duration:

17 d

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Remarks:

and growth

Details on results:

Weights of the single plants after 17 days ranged from 264 mg to 441 mg for the controls and from 125 to 492 mg for the treated plants.
No significant differences between the control and the treated plants were detected.

Any other information on results incl. tables

1. Brassica alba Emergence
- Test:    18/20 = 90 %
- Control: 18/20 = 90 %
Growth
- Test:    179 +/- 46 mg
- Control: 162 +/- 39 mg

2. Lepidum sativum

Emergence
- Test:    18/20 =  90 %
- Control: 20/20 = 100 %
Growth
- Test:    77 +/- 18 mg
- Control: 79 +/- 17 mg

3. Triticum aestivum

Emergence
- Test:    18/20 = 90 %
- Control: 17/20 = 85 %
Growth
- Test:    333 +/- 90 mg
- Control: 344 +/- 52 mg

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

The seedling emergence and the growth of Brassica alba, Lepidium sativum and Triticum aestivum were not affected at the tested concentration (100 mg 2,2,4(or 2,4,4)-trimethyladipic acid/kg dw).

Executive summary:

The study (Hüls AG 1995) assessed effects on seedling emergence and early growth of higher plants following exposure to 2,2,4(or 2,4,4)-trimethyladipic acid in the soil according to OECD TG 208 (1984). Seeds of Brassica alba, Lepidium sativum and Triticum aestivum were placed in contact with soil treated with the test substance (100 mg/kg dry weight) and evaluated for effects after 17 days. No effects on the seedling emergence and the growth of the three test species were observed during this limit test.

The study was assessed as "reliable without restrictions".