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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2005
Report date:
2005

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
no
Qualifier:
according to guideline
Guideline:
ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))
Deviations:
no
GLP compliance:
yes

Test material

Constituent 1
Reference substance name:
Platinum(2+) tetraammine diacetate
Cas Number:
0127733-97-5
IUPAC Name:
Platinum(2+) tetraammine diacetate
Test material form:
solid
Remarks:
crystalline powder

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Duplicate samples for analysis were taken from all test concentrations and the blank control, where 10 mL were taken from the approximate centre of each test vessel at the start and at the end of the test. Prior to analysis, samples were stored at room temperature.
Additionally, singular reserve samples of 10 mL were taken for possible analysis. If not used, these samples were stored at room temperature for a maximum of three months.

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: preparation of test solutions started with a stock solution of 100 mg test item/L. The test substance was completely dissolved in the test medium by careful mixing. The lower test concentrations were prepared by subsequent dilutions of the stock in test medium. The final test solutions were all clear and colourless.
- Controls: test medium without test substance or other additives (blank control)
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): none

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Strain/clone: Straus, 1820
- Source: in-house laboratory culture with a known history
- Further details of test species: at least third generation of species, obtained by acyclical parthenogenesis from a healthy stock, 2nd to 5th brood, showing no signs of stress, presence of males, ephippia or discoloured animals
- Age of parental stock: more than two weeks old
- Age of test organisms: less than 24 hours old
- Feeding during test: none

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Test temperature:
20.7 - 21.1 °C
pH:
7.9 - 8.0
Dissolved oxygen:
8.6 - 8.9 mg/L
Nominal and measured concentrations:
Nominal concentrations: 10, 18, 32, 56 and 100 mg test item/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 100-mL glass test vessels, containing 80 mL test solution
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ISO medium, composed by macro salts (CaCl2.2H2O 293.8 mg/L, MgSO4.7H2O 123.3 mg/L, NaHCO2 64.8 mg/L, KCl 5.8 mg/L) dissolved in tap water purified by reverse osmosis (Milli-RO, Millipore Corp.)
- Intervals of water quality measurement: pH and dissolved oxygen were measured at the beginning and at the end of the test for all concentrations and controls. The temperature of medium was continuously measured in a temperature control vessel from the start of the test.

OTHER TEST CONDITIONS
- Photoperiod: 16 hours of light, daily
- Light intensity: not reported

EFFECT PARAMETERS MEASURED: immobility of daphnids was observed at 24 and 48 hours of exposure.

RANGE-FINDING STUDY
- Test concentrations: 0.1, 1.0, 10, 100 mg test item/L
- Results used to determine the conditions for the definitive study: after 48 hours of exposure all organisms exposed to 100 mg test item/L were immobilised. No daphnids were found to be immobilised at the lower concentrations tested. Hence, the expected 48-hour EC50 was between nominal concentrations of 10 and 100 mg test item/L.
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
20 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% Confidence Interval: 16 - 29 mg test item/L
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
9.75 mg/L
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
Pt
Basis for effect:
mobility
Remarks on result:
other: 95% Confidence Interval: 7.8 - 14.14 mg Pt/L. Pt concentrations calculated based on % Pt in the test material.
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
4.88 mg/L
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
Pt
Basis for effect:
mobility
Remarks on result:
other: Pt concentrations calculated based on % Pt in the test material.
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
86 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% Confidence Interval: 80 - 98 mg test item/L
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
41.93 mg/L
Nominal / measured:
nominal
Conc. based on:
element
Remarks:
Pt
Basis for effect:
mobility
Remarks on result:
other: 95% Confidence Interval: 39 - 47.8 mg Pt/L. Pt concentrations calculated based on % Pt in the test material.
Details on results:
- In the control, no organisms were found to be immobilised or trapped at the surface of water.
Results with reference substance (positive control):
The 48-hour EC50 of potassium dichromate to Daphnia magna was estimated to be 0.63 mg/L with 5 % immobilisation at 0.56 mg/L and 100 % immobilisation at 1.0 mg/L.
Reported statistics and error estimates:
The EC50 values at 24 and 48 hours of exposure were calculated using Probit analysis with the maximum likelihood estimation method (Finney DJ, 1971).

Any other information on results incl. tables

Table 1. Acute immobilisation of Daphnia magna after 24 and 48 hours of exposure to platinum(2 +) tetraammine diacetate

 Nominal concentration (mg test item/L)    Replicate No.    No. of exposed organisms     Response at 24 h     Response at 48 h    
number  total %  number   total % 
 Blank control          1  5  0  0           0  0         
2  5  0  0
3  5  0  0
4  5  0  0
 10          1 5 0  5           0 5         
2 5 1
5 0  0
4 5 0  0
18          1 5 1  5           0 25         
2 5 0  3
3 5 0  2
4 5 0  0
32          1 5 0  5           5 90         
2 5 0  5
3 5 0  5
4 5 1  3
56          1 5 0 0           5 100         
2 5 0  5
3 5 0  5
4 5 0  5
 100          1 5 5  75           5 100         
2 5 3  5
3 5 4  5
4 5 3  5

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Based on nominal concentrations, the 48-hour EC50 of platinum(2+) tetraammine diacetate for Daphnia magna was determined to be 20 mg test item/L corresponding to 9.75 mg Pt/L. The NOEC was determined to be 10 mg test item/L (4.88 mg Pt/L).
Executive summary:

An acute Daphnia magna study was conducted with platinum(2+) tetrammine diacetate. The study is reliable without restrictions, being GLP-compliant and having followed standard test guidelines (OECD 202, ISO 6341 and EU Method C.2).

A static test system was followed. Based on a range-finding test, five test concentrations were used, and these were analysed using ICP-MS. A blank control and a positive control were also tested. Measured concentrations were found to be in agreement with nominal ones, and ranged from 86 to 97 % of nominal. Therefore, nominal concentrations were used for reporting of results. All validity criteria were met and no protocol deviations were recorded. The 48-hour EC50 of platinum(2+) tetraammine diacetate for Daphnia magna was determined to be 20 mg test item/L corresponding to 9.75 mg Pt/L. The NOEC was determined to be 10 mg test item/L (4.88 mg Pt/L).