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Short-term toxicity to fish

The estimated 96 hrs EC50 value of 3-amino-5-hydroxynaphthalene-2,7-disulfonic acid (CAS: 90-40-4 ) on Fathead minnow by three different models i.e, Battery, Leadscope and SciQSAR used within Danish QSAR database is 107.51 mg/L. It can be concluded from the estimated value that the 3-amino-5-hydroxynaphthalene-2,7-disulfonic acid (CAS: 90-40-4 ) is not toxic to aquatic environment and can be cosidered as "not classified" as per CLP regulation.

Short-term toxicity to aquatic invertebrates

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the short term toxicity on aquatic invertebrate predicted for 3-amino-5-hydroxynaphthalene-2,7-disulfonic acid (CAS: 90-40-4). Effect concentration i.e EC50 value estimated to be 116.06 mg/l for Daphnia magna for 48 hrs duration. It can be concluded that the 3-amino-5-hydroxynaphthalene-2,7-disulfonic acid(CAS: 90-40-4) is likely to be not toxic to aquatic invertebrates, hence it can be considered to be “not classified” as per the CLP classification criteria for aquatic environment.

Toxicity to aquatic algae and cyanobacteria

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity on algae was predicted for 3-amino-5-hydroxynaphthalene-2,7-disulfonic acid (CAS: 90-40-4). Effect concentration i.e EC50 value was estimated to be 137.58 mg/l for Desmodesmus subspicatus for 72 hrs duration. It can be concluded that the -amino-5-hydroxynaphthalene-2,7-disulfonic acid (CAS: 90-40-4) was likely to be not-toxic to aquatic algae, hence it can be considered to be “not classified” as per the CLP classification criteria for aquatic environment.

Toxicity to micro-oraganism

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity on micro-oragnisma predicted for 3-amino-5-hydroxynaphthalene-2,7-disulfonic acid (CAS: 90-40-4). Growth inhibiton concentration i.e IGC50 value estimated to be 823.09 mg/l for Tetrahymena pyriformis for 48 hrs duration. It can be concluded that the 3-amino-5-hydroxynaphthalene-2,7-disulfonic acid (CAS: 90-40-4) likely to be not toxic to micRo-organism, hence it can be considered to be “not classified” as per the CLP classification criteria for aquatic environment.

Additional information

Short-term toxicity to fish

Following three studies for the target chemical includes predicted data from Authoritative database and OECD QSAR toolbox to conclude the

3-amino-5-hydroxynaphthalene-2,7-disulfonic acid (CAS: 90-40-4 ) toxicity extent towards the fish are summarized as below:

The estimated 96 hrs EC50 value of 3-amino-5-hydroxynaphthalene-2,7-disulfonic acid (CAS: 90-40-4 ) on Fathead minnow by three different models i.e, Battery, Leadscope and SciQSAR used within Danish QSAR database is 107.51 mg/L. It can be concluded from the estimated value that the 3-amino-5-hydroxynaphthalene-2,7-disulfonic acid (CAS: 90-40-4 ) is not toxic to aquatic environment and can be cosidered as "not classified" as per CLP regulation.

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the short term toxicity on fish predicted for 3-amino-5-hydroxynaphthalene-2,7-disulfonic acid (CAS: 90-40-4). Effect concentration i.e LC50 value estimated to be 109.58 mg/l for fish for 96 hrs duration. It can be concluded that the 3-amino-5-hydroxynaphthalene-2,7-disulfonic acid (CAS: 90-40-4) likely to be toxic to fish, hence it can be considered to be “not classified” as per the CLP classification criteria for aquatic environment.

The above predicted study is suoorted by the experimental result of read across Sulphanilic acid (CAS: 121-57-3) from Chemospheres 1994, suggests that short-term toxicity to fish test was carried out for to study the effect of Sulphanilic acid on aquatic environment.

When Sulphanilic acid is exposed to thePimephales promelas for 96 h under static condition. Mortality was considered for the measurement of effect in 50 % of population of fish. The Lethal concentration to 50% LC50 ofPimephales promelas at 96 h is 100.4 mg/L. It can be concluded from the value that the Sulphanilic acid is not toxic to the aquatic environment and can be considred as ‘not classified” as per CLP regulation.

Thus based on the effect concentrations which is in the range 100.4 mg/l to 109.58 mg/l give the conclusion that test substance 3-amino-5-hydroxynaphthalene-2,7-disulfonic acid (CAS: 90-40-4 ) was likely to be not toxic to fish at environmentally relevant concentrations and can be considered to benot classifiedas per the CLP classification criteria.

Short-term toxicity to aquatic invertebrates

Following two studies for the target chemical and read across chemical includes predicted data from the OECD QSAR toolbox and experimental data to conclude the 3-amino-5-hydroxynaphthalene-2,7-disulfonic acid (CAS: 90-40-4 ) toxicity extent towards the aquatic invertebrate are summarized as below:

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the short term toxicity on aquatic invertebrate predicted for 3-amino-5-hydroxynaphthalene-2,7-disulfonic acid (CAS: 90-40-4). Effect concentration i.e EC50 value estimated to be 116.06 mg/l for Daphnia magna for 48 hrs duration. It can be concluded that the 3-amino-5-hydroxynaphthalene-2,7-disulfonic acid(CAS: 90-40-4) is likely to be not toxic to aquatic invertebrates, hence it can be considered to be “not classified” as per the CLP classification criteria for aquatic environment.

The above predicted study is supported by the experimental data of read across chemical Sulphanilic acid (CAS: 121-57-3) from Chemosphere 1994, indicates that the effective concentration to 50 % of daphnia magna at 24 h for Sulphanilic acid is 109.13 mg/L .It can concluded from the value that the Sulphanilic acid is not toxic to the aquatic environment.

Thus based on the effect concentrations which is in the range 109.13 mg/L to116.06 mg/lgive the conclusion that test substance3-amino-5-hydroxynaphthalene-2,7-disulfonic acid (CAS: 90-40-4)was likely to be non-toxic to aquatic inverbrate at environmentally relevant concentrations and can be considered to be “not classified” as per the CLP classification criteria.

Toxicity to aquatic algae and cyanobacteria

Following four studies for the target chemical and read across chemical includes predicted data from the OECD QSAR toolbox and experimental data to conclude the 3-amino-5-hydroxynaphthalene-2,7-disulfonic acid (CAS: 90-40-4 ) toxicity extent towards the aquatic algae are summarized as below:

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity on algae was predicted for 3-amino-5-hydroxynaphthalene-2,7-disulfonic acid (CAS: 90-40-4). Effect concentration i.e EC50 value was estimated to be 137.58 mg/l for Desmodesmus subspicatus for 72 hrs duration. It can be concluded that the -amino-5-hydroxynaphthalene-2,7-disulfonic acid (CAS: 90-40-4) was likely to be not-toxic to aquatic algae, hence it can be considered to be “not classified” as per the CLP classification criteria for aquatic environment.

Similar predicted study is done by Using three different models i.e, Battery, Leadscope and SciQSAR used within Danish QSAR database, the effective concentration (EC50) at 72 hrs for 3-amino-5-hydroxynaphthalene-2,7-disulfonic acid (CAS: 90-40-4) in Pseudokirchneriella s. is estimated to be 16789.04 mg/L. On the basis of the estimated value it can be concluded that the 3-amino-5-hydroxynaphthalene-2,7-disulfonic acid (CAS: 90-40-4) is not toxic toxic to the aquatic environment.

The above predicted study is supported by the experimental data of read across 3-amino-4-hydroxybenzenesulphonic acid (CAS:98 -37 -3) from Water Reseach 1968, suggests that the No observable effect concentration NOEC toChlorella pyrenoidosa at 72 hr is 1000 mg/l for 3-amino-4-hydroxybenzenesulphonic acid. It can be concluded for the value it can be concluded that the 3-amino-4-hydroxybenzenesulphonic acid is not toxic to the aquatic environment and can be considered as “not classified” as per CLP regulation.

Another experimental data for read across4-aminobenzoic acid (CAS: 150 -13 -0) from Journal of Hazardous Materials 2009, indicates that the

Toxicity to aquatic algae and cyanobacteria test was carried out for 48 Hour(s) to study the effect of 4-aminobenzoic acid on aquatic environment at temp 24 C and pH 6.5.

The Effective concentration EC50 to 50% of Pseudokirchneriella subcapitata for 4-aminobenzoic acid at 48 h is 199.9 mg/L. Decreasing Growth rate was measured as effect. It can be concluded from the value that the 4-aminobenzoic acid is not toxic to the aquatic environment and can be considered as “not classified’ as per CLP regulation.

Thus based on the effect concentrations which is in the range 137.58 mg/l to16789.04 mg/lgive the conclusion that test substance3-amino-5-hydroxynaphthalene-2,7-disulfonic acid (CAS: 90-40-4)was likely to be non-toxic to aquatic algae at environmentally relevant concentrations and can be considered to be “not classified” as per the CLP classification criteria.

Toxicity to micro-organism

Following two studies for the target chemical and read across chemical includes predicted data from the OECD QSAR toolbox and experimental data to conclude the 3-amino-5-hydroxynaphthalene-2,7-disulfonic acid (CAS: 90-40-4 ) toxicity extent towards the aquatic invertebrate are summarized as below:

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity on micro-oragnisma predicted for 3-amino-5-hydroxynaphthalene-2,7-disulfonic acid (CAS: 90-40-4). Growth inhibiton concentration i.e IGC50 value estimated to be 823.09 mg/l for Tetrahymena pyriformis for 48 hrs duration. It can be concluded that the 3-amino-5-hydroxynaphthalene-2,7-disulfonic acid (CAS: 90-40-4) likely to be not toxic to micRo-organism, hence it can be considered to be “not classified” as per the CLP classification criteria for aquatic environment.

The above predicted data is supported by the experimental study Sulphanilic acid (CAS: 121-57-3) from Chemosphere 1994, suggests that the effective concentration EC0 to bacteria is 10000 mg/l at 24h for Sulphanilic acid. It can be concluded form the value that the Sulphanilic acid is not toxic to the aquatic environment and can be considered as “not classified” as per CLP regulation.

Thus based on the effect concentrations which is in the range 823.09 mg/l to10000 mg/lgive the conclusion that test substance3-amino-5-hydroxynaphthalene-2,7-disulfonic acid (CAS: 90-40-4)was likely to be non-toxic to micro-organism at environmentally relevant concentrations and can be considered to be “not classified” as per the CLP classification criteria.