Alkylbenzenesulphonic acid compound with alkanolamine

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Remarks:

the tested substance is a structural analogue of the salt

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study well documented, meets generally accepted scientific principles, acceptable for assessment

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Principles of method if other than guideline:

The purpose of the study was to determine the toxicity of five purehomologues of LAS to the activated sludge of a treatment plant basically operating on domestic sewage. A contact time of 3 hours instead of 15 minutes was chosen to better simulate the real residence time used in wastewater treatment plants (4-6 hours).

GLP compliance:

not specified

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

activated sludge

Test type:

other: aquatic

Limit test:

no

Total exposure duration:

3 h

Details on test conditions:

Activated sludges:The activated sludge from the aeration pond of the sewage treatment plant of Estepona. This sludge has a carbon content of 40% and moisture level of 82%.

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol

Key result

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

500 - 723 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Details on results:

Acute toxicity to Microorganism (activated sludge): 3hr-EC50 is 500-723 mg/L (nominal).

Results with reference substance (positive control):

The 3-hour EC50 range for the reference substance (3,5-dichlorophenol) ranged from 20- 30 mg/L, within the valid range of 5-30 mg/L

Validity criteria fulfilled:

yes

Conclusions:

Acute toxicity to Microorganism (activated sludge): 3hr-EC50 is 500-723 mg/L (nominal).

Reference 2

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Remarks:

the tested substance is the amine part of the compound

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Data from reliable handbook

Principles of method if other than guideline:

Growth inhibition test

GLP compliance:

no

Test organisms (species):

Pseudomonas putida

Test type:

static

Water media type:

freshwater

Duration:

17 h

Dose descriptor:

other: TTC

Effect conc.:

15 000 mg/L

Description of key information

The toxicity of the acid and amine towards micro organisms as present in waste water treatment systems is low. The acid has the lowest EC50 of 500 mg/L (Verge & Moreno 1996). The value for the amine is recalculated from a handbook value.

500 mg/L will be taken as starting point for the PNEC calculation of the salt.

Key value for chemical safety assessment

EC50 for microorganisms:

500 mg/L