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Ecotoxicological information

Toxicity to microorganisms

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Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Remarks:
the tested substance is a structural analogue of the salt
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study well documented, meets generally accepted scientific principles, acceptable for assessment
Qualifier:
according to
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Principles of method if other than guideline:
The purpose of the study was to determine the toxicity of five purehomologues of LAS to the activated sludge of a treatment plant basically operating on domestic sewage. A contact time of 3 hours instead of 15 minutes was chosen to better simulate the real residence time used in wastewater treatment plants (4-6 hours).
GLP compliance:
not specified
Analytical monitoring:
not specified
Vehicle:
not specified
Test organisms (species):
activated sludge
Test type:
other: aquatic
Limit test:
no
Total exposure duration:
3 h
Details on test conditions:
Activated sludges:The activated sludge from the aeration pond of the sewage treatment plant of Estepona. This sludge has a carbon content of 40% and moisture level of 82%.
Reference substance (positive control):
yes
Remarks:
3,5-dichlorophenol
Key result
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
500 - 723 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Details on results:
Acute toxicity to Microorganism (activated sludge): 3hr-EC50 is 500-723 mg/L (nominal).
Results with reference substance (positive control):
The 3-hour EC50 range for the reference substance (3,5-dichlorophenol) ranged from 20- 30 mg/L, within the valid range of 5-30 mg/L
Validity criteria fulfilled:
yes
Conclusions:
Acute toxicity to Microorganism (activated sludge): 3hr-EC50 is 500-723 mg/L (nominal).
Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Remarks:
the tested substance is the amine part of the compound
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Data from reliable handbook
Principles of method if other than guideline:
Growth inhibition test
GLP compliance:
no
Test organisms (species):
Pseudomonas putida
Test type:
static
Water media type:
freshwater
Duration:
17 h
Dose descriptor:
other: TTC
Effect conc.:
15 000 mg/L

Description of key information

The toxicity of the acid and amine towards micro organisms as present in waste water treatment systems is low. The acid has the lowest EC50 of 500 mg/L (Verge & Moreno 1996). The value for the amine is recalculated from a handbook value.

500 mg/L will be taken as starting point for the PNEC calculation of the salt.

Key value for chemical safety assessment

EC50 for microorganisms:
500 mg/L

Additional information