Alkylbenzenesulphonic acid compound with alkanolamine

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Remarks:

the tested substance represents the anionic part of the salt

Adequacy of study:

key study

Study period:

May 8, 2000

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study well documented, meets generally accepted scientific principles, acceptable for assessment

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

Species: Daphnia magna, <24 h old.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

20±1 degree C

pH:

pH 7.8-8.0

Dissolved oxygen:

dissolved oxygen 7.7-8.0;

Nominal and measured concentrations:

0.4, 0.7, 1.2, 2.0, 3.5, 6.0, and 10 mg/L

Details on test conditions:

Seven concentrations and a control were tested (0.4, 0.7, 1.2, 2.0, 3.5, 6.0, and 10 mg/L). Physicochemical parameters were maintained within acceptable ranges (temperature 20±1 degree C; dissolved oxygen 7.7-8.0; pH 7.8-8.0). At 48 hours, 0/20 daphnids were immobile in the 0.4 through 3.5 mg/L concentrations, 14/20 in 6.0 mg/L, and 20/20 in 10 mg/L. Graphical interpolation was used to calculate the EC50 value.

Reference substance (positive control):

not required

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

5.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: (95% 3.5-6.0 mg/L)

Details on results:

A 48 h EC50 of 5.2 mg/L has been determined for the effects of this substance on Immobility of the Daphnia magna.

Validity criteria fulfilled:

yes

Conclusions:

A 48 h EC50 of 5.2 mg/L has been determined for the effects of this substance on Immobility of the Daphnia magna.

Executive summary:

A 48 h EC50 of 5.2 mg/L has been determined for the effects of this substance on Immobility of the Daphnia magna.

Reference 2

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Remarks:

the tested substance represents the anionic part of the salt

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study well documented, meets generally accepted scientific principles, acceptable for assessment

Qualifier:

no guideline followed

Principles of method if other than guideline:

The effects of four concentrations of dodecyl benzene sulfonate at four salinity levels on adult Tisbe bulbisetosa were studied in laboratory experiments. The DBS solutions were analysed by the methylene method to control their stability with time.
The stock solution has been prepared by dissolving 100 mg of pure DBS to 100 mL of distilled water. Test solutions for this test have been obtained both by diluting 3, 6, 9, 12 mL of the stock solution in filtered seawater at a determined level of salinity to 1000 mL, and by diluting the fixed amount stock solution in seawater of 9, 12, 18, 36‰ salinity.

GLP compliance:

not specified

Analytical monitoring:

yes

Vehicle:

no

Details on test solutions:

The stock solution has been prepared by dissolving 100 mg of pure DBS to 100 mL of distilled water. Test solutions for this test have been obtained both by diluting 3, 6, 9, 12 mL of the stock solution in filtered seawater at a determined level of salinity to 1000 mL, and by diluting the fixed amount stock solution in seawater of 9, 12, 18, 36‰ salinity.

Test organisms (species):

other aquatic crustacea: Tisbe bulbisetosa

Details on test organisms:

Tisbe bulbisetosa : adult female

Test type:

not specified

Water media type:

saltwater

Limit test:

no

Total exposure duration:

48 h

Nominal and measured concentrations:

This study is the combined effects of four concentrations (0, 3, 6, 9, 12 ppm) of DBS at four salinity (9, 12, 18, 360) on adult Tisbe bulbisetosa.

Reference substance (positive control):

not required

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

4.58 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: (18‰ salinity) = 4.58 ppm(95% confidence interval: 4.00 to 5.24)

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

5.12 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: (36‰ salinity) = 5.12 ppm(95% confidence interval: 4.27 to 6.13)-

Details on results:

- 48 hr-LC50 (36‰ salinity) = 5.12 ppm(95% confidence interval: 4.27 to 6.13)- 48 hr-LC50 (18‰ salinity) = 4.58 ppm(95% confidence interval: 4.00 to 5.24)

A 48 h LC50 of 4.58 mg/L (18‰ salinity) and 5.1 mg/L (36‰ salinity) has been determined for the effects of the substance on mortality of the Tisbe bulbisetosa.

Validity criteria fulfilled:

yes

Conclusions:

A 48 h LC50 of 4.58 mg/L (18‰ salinity) and 5.1 mg/L (36‰ salinity) has been determined for the effects of the substance on mortality of the Tisbe bulbisetosa.

Description of key information

For the acid the 48h EC50 value reported in Daphnia magna was 5.2 mg/L (1995). In addition, a 48 h EC50 of 4.58 mg/L (18‰ salinity) and 5.1 mg/L (36‰ salinity) has been determined in the salt water species Tisbe bulbisetosa (1980).

The 48 h EC50 for the amine was 277.7 mg/L (BASF 1988). As the representative for the anionic part of the salt showed a higher toxicity compared to the representative for the cationic part.

The EC50 of 5.2 mg/L is chosen to represent the toxicity of the salt in a reasonable worst case approach.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

5.2 mg/L

Marine water invertebrates

Marine water invertebrates

Effect concentration:

4.58 mg/L

Additional information

48 Hour EC50 values for the acid in daphnia magna ranged from 3.5 to 5.9 mg/L