Alkylbenzenesulphonic acid compound with alkanolamine

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Remarks:

the tested substance is the amine component of the compound

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

GLP compliance:

yes

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Duration of test (contact time):

28 d

Initial conc.:

52.6 mg/L

Based on:

other: ThOD NH3

Parameter followed for biodegradation estimation:

O2 consumption

Key result

Parameter:

% degradation (O2 consumption)

Value:

94

Sampling time:

28 d

Interpretation of results:

readily biodegradable

Reference 2

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Remarks:

the tested substance is the acid component of the compound

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Remarks:

the information in the publication is limited to what is included in the summary, test substance identification not completely clear

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

GLP compliance:

not specified

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

The activated sludge sample was obtained from the pilot-plant during period A in this study.

Duration of test (contact time):

28 d

Initial conc.:

25.1 mg/L

Based on:

test mat.

Details on study design:

This test was carried out according to the guidelines of OECD test method encoded 301F. An automated sapromate(Voith) was used to follow O2 consumption in response to LAS mineralisation. A test with a blank inoculm was included as well as a test with 109mg/l of the easily biodegradable potassium hydrogen phthalate (PHP) to serve as a method control. All the tests were carried out in duplicate over a period of 28 days at a temperature of 20 deg C.

Reference substance:

other: potassium hydrogen phthalate (PHP)

Key result

Parameter:

% degradation (O2 consumption)

Value:

> 60

Remarks on result:

other: within 28days

Details on results:

The O2-consumption data generated in the ready biodegradability tests(data not shown) showed that LAS is readily biodegradable as defined by the OECD Guidelines: more than 60% of mineralisation was achieved within 28 days and this level was reached within a time windows of 10 days from the start of mineralisation. From the O2-consumption data a biomass-yield Y of 0.50 (+/-) 0.02 mgO2/mgO2 and a maximum specific growth rate of 0.050 (+/-) 0.003/h were estimated.

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

Data of following studies are demonstrating rapid biodegradation of C12- LAS (including Na-LAS) under aerobic and anaerobic conditions. The result is that more than 60% of mineralisation was achieved within 28 days.

Executive summary:

Test substance is readily biodegradable (more than 60% by mineralization wihin 28days) [OECD TG 301 F].

Reference 3

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Remarks:

the tested substance is the acid component of the compound

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

Study well documented, meets generally accepted scientific principles, acceptable for assessment . Sodium dodecylbenzenesulfonate (CAS No. 25155-30-0, EC Number; 246-680-4) is a very close analogue of the dissociated acid because it readily dissociates in water and release the dodecylbenzene sulfonic anion in solution. No information on the purrity/composition of the tested sample

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 301 E (Ready biodegradability: Modified OECD Screening Test)

GLP compliance:

not specified

Oxygen conditions:

aerobic

Inoculum or test system:

natural water

Details on inoculum:

To each liter of sterile Bay water was added 1.0 mL of sterile BOD buffer and 5 mL of a “seed” culture. The bacterial “seed” consisted of the sample of (non-filtered) Bay water from May 30, 1972 which was supplemented with DBS (10 ppm) and incubated at 20 deg C for 17 days, by which time more than 75% degradation had occurred.

Duration of test (contact time):

17 d

Initial conc.:

10 other: ppm

Based on:

test mat.

Details on study design:

Detergent decomposition depends on aerobic respiratory processes, incubation in a closed vessel results in depletion of dissolved oxygen. To determine oxygen utilization during detergent oxidation a BOD-type test was employed with sodium dodecylbenzene sulfonate as substrate. Bay water aged more than 30 days was sterilized by membrane filtration (Millipore, 0.45 um).

Key result

Parameter:

% degradation (test mat. analysis)

Value:

> 75

Sampling time:

11 d

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

Biodegradation of the test substance, at 10 ppm was measured 75% after 11 days and test temperature was maintained at 20 deg C for 17 days. The test substance could be conclued as readily degradable.

Description of key information

Temmink and Klapwijk (2004) conducted OECD 301F test on the acid and found that more than 60% mineralisation was achieved within 28 days. This finding is in agreement with the results of a study on the Na-salt of the acid (OECD 201E) which gave a degradation of > 75% in 11 days.

In a test by Dow (1988) of the amine part according to OECD 301F, a degradation rate of 94% was found after 28 days exposure.

Key value for chemical safety assessment

Biodegradation in water:

readily biodegradable

Additional information

The substances tested are the acid and amine parts of the salt. It can therefore be concluded that the compound is readily biodegradable.